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URL: http://www.addgene.org/24501
Proper Citation: RRID:Addgene_24501
Insert Name: Gq alpha
Organism: Mus musculus
Bacterial Resistance: Ampicillin and Tetracycline
Defining Citation: PMID:8387644
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Comments: This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Gi alpha residues (EYNLV to DCGLF). This construct allows many Gi-coupled receptors to stimulate phospholipase C (PLC). This is the most popular chimera, perhaps because it is easier to talk about coupling to Gi-coupled receptors with "qi5" rather than "qo5" or "qz5." Contains an internal hemaglutinin epitope tag (DVPDYA) that has no effect on receptor coupling. Please see supplemental document for G-protein chimera user manual.
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Source: Addgene
