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Plasmid Name
LSB-hsa-miR-514a-5p
RRID:Addgene_103598 RRID Copied  
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RRID:Addgene_103598
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Plasmid Information

URL: http://www.addgene.org/103598

Proper Citation: RRID:Addgene_103598

Insert Name: hsa-miR-514a-5p target

Organism: Homo sapiens

Bacterial Resistance: Ampicillin and Kanamycin

Defining Citation: PMID:29934631

Vector Backbone Description: Vector Backbone:Low Sensor Backbone (LSB); Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin

Comments: Please note that this plasmid is inherently unstable due to a repeating poly A element. We recommend performing a diagnostic digest on multiple colonies upon receipt. The depositors would like to note the presence of several discrepancies between the depositor sequence and physical plasmid sequence. In particular, the sequences upstream and downstream of the puromycin coding region have 10 bp insertions which do not affect the ability to perform puromycin selection. The sequences for those regions within the physical plasmids are as follows: Upstream of Puro: GAATTCGACCGCCTGTCTCAAGGTGCCACC; Downstream of Puro: GCTTTGAGACTACGCGTGAATTCACTCCTC.

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Ratings and Alerts

No rating or validation information has been found for LSB-hsa-miR-514a-5p.

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Data and Source Information

Source: Addgene