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URL: http://www.addgene.org/87344
Proper Citation: RRID:Addgene_87344
Insert Name: TRIC-B1
Organism: Caenorhabditis elegans
Bacterial Resistance: Bleocin (Zeocin)
Defining Citation: PMID:27698420
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3221; Vector Backbone:pPICZ A; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Comments: Vector pPICZ A and insert sequence were modified as follows: 1. Restriction sites including Pml I; Sfi I; BsmB I; Asp718 I; Kpn I were destroyed during cloning full-length CeTRIC-B1 to the vector. 2. In the C-terminal truncation, the sequence between XhoI and polyhistidine had been deleted. That is to say, restriction sites including Xho I, Sac II, Not I, Apa I, and myc-epitope, were destroyed during truncation. 3. A kozak suqence "ACC" was inserted between the restriction site "EcoR I" and initial codon, we added these 3 bp into insert sequence size. In summary, the size of pPICZ A backbone is changed from 3329 bp to be 3221 bp.
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Source: Addgene
