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URL: http://www.addgene.org/40248
Proper Citation: RRID:Addgene_40248
Insert Name: One Step Cloneing Vector for Overexpression
Organism: Synechococcus elongatus PCC 7942
Bacterial Resistance: Spectinomycin and Streptomycin
Defining Citation: PMID:15775978
Vector Backbone Description: Vector Backbone:pAM2990; Vector Types:Other, Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin
Comments: pAM2990 was partially digested with SacI and re-ligatedto remove the antihel fragment. The net result is the insertion of the BglII fragment from pAM2255 into BamHI digested pAM1303 in the orientation that Ptrc goes away from the SacI site in pAM1303 (SacI digestion gives a 1.7 KB band). This plasmid can be used as a one-step cloning vector for overexpression.
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Source: Addgene
