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URL: http://www.addgene.org/12558
Proper Citation: RRID:Addgene_12558
Insert Name: CCAAT/Enhancer-binding Protein beta
Organism: Rattus norvegicus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5427; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The C/EBPbeta gene encodes 3 in-frame translational isoforms (LAP*, LAP, and LIP). LAP is the predominant activating isoform expressed in most cells, and this plasmid is designed to express the LAP isoform of the protein. The construct does not include the extra 21 codons at the N terminus that are present in LAP*. The complete insert (not just the ORF) includes a Kozak box sequence at the N terminus. For more information on the C/EBPbeta isoforms, please see the original reference: Descombes, P., and Schibler, U. A liver-enriched transcriptional activator protein, LAP, and a transcriptional inhibitory protein, LIP, are translated from the same mRNA. Cell 67:569-579 (1991).
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No rating or validation information has been found for pcDNA 3.1(-) rat C/EBP beta (LAP).
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Source: Addgene
