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URL: http://www.addgene.org/117480
Proper Citation: RRID:Addgene_117480
Insert Name: uracil phosphoribosyltransferase
Organism: Other
Bacterial Resistance: Ampicillin and Kanamycin
Defining Citation: PMID:23393089
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCR4/TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Selection for kanamycin resistance after introduction of pMO746 identifies those cells in which integration into the chromosome of part or all of the plasmid has occurred. Kanamycin resistance is a strong selection in the sulfate-reducing bacteria (SRB). Ampicillin is not universally effective in the SRB but is often used for selection in Escherichia coli. Thus this plasmid can be readily grown in E. coli but is actually unstable in the SRB.
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Source: Addgene
