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URL: http://www.addgene.org/11362
Proper Citation: RRID:Addgene_11362
Insert Name: IRS-3
Organism: Rattus norvegicus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:11113178
Vector Backbone Description: Backbone Marker:pBABE-puro is available at Addgene; Backbone Size:5169; Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: Rat IRS-3 was generated by PCR from the rat genomic DNA sequence. The intron was removed by PCR overlap extension using the following two pairs of primers: 5'-CGTGGATCCGCGATGAAGCCTGCAGGTACG-3' (sense) plus 5'-CTTGGGGGCTGAAACCCATGTTTGCTGGGCA-3' (antisense) and 5'-TGCCCAGCAAACATGGGTTTCAGCCCCCAAG-3' (sense) plus 5'-GCTGTCGACGTTCTAGAACTTGATGCTG-3' (antisense). BamHI and SalI sites were introduced at the 5' and 3' ends, respectively, of the coding sequence of IRS-3 by PCR. The subsequently generated coding sequence of rat IRS-3 was ligated into BamHI and SalI sites in pBABE-puro.
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No rating or validation information has been found for pBABE puro rat IRS-3.
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Source: Addgene
