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URL: http://www.addgene.org/107770
Proper Citation: RRID:Addgene_107770
Insert Name: Che9C RecT
Organism: Other
Bacterial Resistance: Chloramphenicol and Kanamycin
Defining Citation: PMID:25779316
Vector Backbone Description: Backbone Marker:Sabine Ehrt and Dirk Schnappinger; Backbone Size:8026; Vector Backbone:pUV15tet-O; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Comments: Induced with anhydrotetracycline, the plasmid can be used to introduce single base pair changes in the chromosomes of M. smegmatis and M. tuberculosis. For original description of methodology, see: van Kessel, J.C. & Hatfull, G.F. Efficient point mutagenesis in mycobacteria using single-stranded DNA recombineering: characterization of antimycobacterial drug targets. Mol Microbiol 67, 1094-1107 (2008).
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Source: Addgene
