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https://www.brown.edu/research/projects/superfund/cores/core-d

Core provides equipment and technical expertise for evaluation of molecular and morphological changes in cells, tissues, and organs following exposure to complex environmental contaminants. Provides equipment, including automated tissue processor, paraffin embedding center, two automated microtomes, cryostat, vibratome for soft-tissue sectioning, multiheaded light microscope with projection capabilities, system for laser capture microdissection, and slide scanner with analysis software for identification and quantification of morphological structures.Offers expertise in histopathological and immunocytochemial methods, including fixation, dehydration, embedding, sectioning, histological staining, immunolabeling, high-resolution imaging, and quantitative image analysis. Services in sample preparation, offers assistance in imaging and image analysis and provides consultation for ongoing or future research projects, training program for students and investigators who use centrally available equipment.

Proper citation: Brown University Molecular Pathology Core Facility (RRID:SCR_017898) Copy   

•   Source: SciCrunch Registry

https://www.igb.illinois.edu/corefacilities

Core for biological microscopy and image analysis. Offers high-end equipment,user training,ongoing support, including experiment design and data interpretation,twenty-four hour access Services,3D Printing, Transfer Files, Image Analysis,Histology,Instrument Training.

Proper citation: University of Illinois at Urbana-Champaign Core Facilities at IGB (RRID:SCR_017938) Copy   

•   Source: SciCrunch Registry

https://www.microscopyu.com/museum/eclipse-e600

Microscope equipped with CFI60 infinity optical system, providing images in all applications. Incorporates specifications adopted for CFI60 series objectives, including 60 millimeter parfocal distance, 25 millimeter thread size, and standard 22 millimeter field of view. Main components of CFI60 infinity optical system include objective, tube lens to converge light beam, and eyepiece lens to enlarge intermediate image. Fluorescence microscope that has detachable substage, 12 volt 100 watt tungsten halide lamp, filter magazine, and choice of sextuple nosepiece or sextuple DIC nosepiece.Has objectives for brightfield, darkfield, Nomarski DIC, epi fluorescence, or phase contrast techniques.

Proper citation: Nikon Eclipse E600 Fluorescence Microscope (RRID:SCR_018606) Copy   

•   Source: SciCrunch Registry

https://biomedicalsciences.unimelb.edu.au/departments/department-of-biochemistry-and-pharmacology/research/services/bomp

Core provides access to fluorescence microscopy equipment including Deltavision OMX Blaze Super Resolution (3D-SIM/SMLM), Leica SP8 inverted confocal, La Vision Ultramicroscope II Lightsheet Microscope,Nikon Andor WD spinning disk, Nikon A1R inverted confocal x2, Olympus FV-MPERS Multi Photon,Olympus FV3000 inverted confocal (live cell) ISS Fast-FLIM, Perkin Elmer Operetta High Content Imaging, Zeiss LSM800 upright Airyscan, Zeiss LSM880 upright Airyscan fast, Zeiss Elyra/LSM880 inverted (live cell), super resolution (Airyscan/SMLM). Staff can help with image analysis, super resolution microscopy, fluorescence lifetime imaging microscopy, high content imaging and light sheet microscopy techniques.

Proper citation: University of Melbourne Biological Optical Microscopy Platform (BOMP) Core Facility (RRID:SCR_018888) Copy   

•   Source: SciCrunch Registry

http://www.pstnet.com/software.cfm?ID=96

Designed for use in an MRI simulator, MoTrak software uses Ascension Technology?s Flock of Birds. The sensor attaches to the subject?s head and determines the position of the head in space relative to the transmitter. The sensor records angular rotations as well as positional displacements from an initially calibrated position. This information is displayed and logged by the program in real-time, allowing observation of head motion in an MRI simulator. In the simulator, the participant can simultaneously be habituated to the MRI environment, while being trained to remain still via feedback from the MoTrak system.

Proper citation: MoTrak Head Motion Tracking System (RRID:SCR_009607) Copy   

•   Source: SciCrunch Registry

http://edboyden.org/05.09.boyden.html

Laser tool that enables neurons to be optically silenced by pulses of yellow light, the light-activated chloride pump halorhodopsin (Halo), in a paper entitled Multiple-color optical activation, silencing, and desynchronization of neural activity, with single-spike temporal resolution. Temporally precise, noninvasive control of activity in well-defined neuronal populations is a long-sought goal of systems neuroscience. We adapted for this purpose the naturally occurring algal protein Channelrhodopsin-2, a rapidly gated light-sensitive cation channel, by using lentiviral gene delivery in combination with high-speed optical switching to photostimulate mammalian neurons. We demonstrate reliable, millisecond-timescale control of neuronal spiking, as well as control of excitatory and inhibitory synaptic transmission. This technology allows the use of light to alter neural processing at the level of single spikes and synaptic events, yielding a widely applicable tool for neuroscientists and biomedical engineers. The quest to determine how precise neural activity patterns mediate computation, behavior, and pathology would be greatly aided by a set of tools for reliably activating and inactivating genetically targeted neurons, in a temporally precise and rapidly reversible fashion. Having earlier adapted a light-activated cation channel, 1channelrhodopsin-2 (ChR2), for allowing neurons to be stimulated by blue light, we searched for a complementary tool that would enable optical neuronal inhibition, driven by light of a second color. Here we report that targeting the 1codon-optimized form of the light-driven chloride pump halorhodopsin from the archaebacterium Natronomas pharaonis (hereafter abbreviated Halo) to genetically-specified neurons enables them to be silenced reliably, and reversibly, by millisecond-timescale pulses of yellow light. We show that trains of yellow and blue light pulses can drive high-fidelity sequences of hyperpolarizations and depolarizations in neurons simultaneously expressing yellow light-driven Halo and blue light-driven ChR2, allowing for the first time manipulations of neural synchrony without perturbation of other parameters such as spiking rates. The Halo/ChR2 system thus constitutes a powerful toolbox for multichannel photoinhibition and photostimulation of virally or transgenically targeted neural circuits without need for exogenous chemicals, enabling systematic analysis and engineering of the brain, and quantitative bioengineering of excitable cells.

Proper citation: Channelrhodopsin-2 enables optical activation of neurons (RRID:SCR_008833) Copy   

•   Source: SciCrunch Registry

https://www.agilent.com/en/product/automated-electrophoresis/tapestation-systems/tapestation-instruments/4200-tapestation-system-228263

Benchtop genetic analyzer. Electrophoresis system for DNA and RNA sample quality control. It can be used for next generation sequencing or Biobank workflow.

Proper citation: Agilent 4200 TapeStation System (RRID:SCR_018435) Copy   

•   Source: SciCrunch Registry

http://tools.thermofisher.com/content/sfs/manuals/cms_040970.pdf

Automated PCR instrument for automated amplification of nucleic acids with Polymerase Chain Reaction. It has reaction volumes of up to 50 uL and sample temperature range of 4 to 99.9 C.

Proper citation: Applied Biosystems GeneAmp 9700 PCR Thermocycler System (RRID:SCR_018436) Copy   

•   Source: SciCrunch Registry

https://www.bdbiosciences.com/en-us/products/instruments/flow-cytometers/research-cell-analyzers/bd-facsymphony-a3

Fluorescence activated cell analyzing instrument by BD Biosciences. Allows for the simultaneous detection of up to 30 parameters and 28 colors.

Proper citation: BD FACSymphony A3 Cell Analyzer (RRID:SCR_023644) Copy   

•   Source: SciCrunch Registry

https://download.cytivalifesciences.com/cellanalysis/download_data/incell/incell_docs/IN%20Cell%20Analyzer%202200%20User%20Manual.pdf

Cell imaging system for fast, automated imaging in fixed and live cells. Instrument can rapidly image cells using laser autofocusing, LED light source and CMOS camera.

Proper citation: GE Healthcare IN Cell Analyzer 2200 (RRID:SCR_023616) Copy   

•   Source: SciCrunch Registry

https://www.illumina.com/systems/sequencing-platforms/nextseq-1000-2000.html

Sequencing system supports range of methods such as exome sequencing, target enrichment, single-cell profiling.

Proper citation: Illumina NextSeq 2000 system (RRID:SCR_023614) Copy   

•   Source: SciCrunch Registry

https://www.ionpath.com/mibiscope/

Imaging platform, enabling phenotypic profiling and spatial analysis of tissue microenvironment. Enables over 40 markers to be visualized simultaneously with single step staining and single step imaging.

Proper citation: Ionpath MIBIscope System (RRID:SCR_023613) Copy   

•   Source: SciCrunch Registry

https://www.htximaging.com/htx-m5-sprayer

Sprayer builds on capabilities of HTX TM-Sprayer providing innovative M5 chassis, high velocity stage and heated sample holder drawer. Sample plate holder is mounted on drawer mechanism to allow easy loading of samples, better protection of user and better alignment of various plate types. New High-Velocity XY stage allows nozzle displacement speed of up to 5,600mm/min and expands process range. Super dry spray can be achieved at high flow rates of solvent further improving instrument performance. E/Z trough gutter design improves chemical containement and is easy to remove and clean. Bulkhead connectors are directly mounted on it to also allow easy access to in line filter.

Proper citation: HTX M5 Sprayer (RRID:SCR_023733) Copy   

•   Source: SciCrunch Registry

https://andor.oxinst.cn/products/scmos-camera-series/zyla-4-2-scmos

Zyla 4.2 PLUS is the latest sCMOS technology advancement from Andor. At its heart, Zyla 4.2 PLUS benefits from latest generation sCMOS sensor technology, delivering a further 10 % boost in QE, resulting in an unprecedented 82% QEmax.

Proper citation: Andor Zyla 4.2 sCMOS (RRID:SCR_023610) Copy   

•   Source: SciCrunch Registry

https://www.thermofisher.com/us/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-tribrid-mass-spectrometers/orbitrap-eclipse-tribrid-mass-spectrometer.html

Mass spectrometer designed for most difficult analytical challenges. Incorporates inventions in ion transmission and control, extended m/z range, and real-time decision making. System suited for proteomics, structural biology, small-molecule, and biopharmaceutical characterization experiments.

Proper citation: Thermo Scientific Orbitrap Eclipse Tribrid mass spectrometer (RRID:SCR_023618) Copy   

•   Source: SciCrunch Registry

https://www.micro-shop.zeiss.com/en/us/system/axio+observer-axio+observer+3-inverted+microscopes/10301/

Inverted microscope Axio Observer 3 for transmitted light brightfield LED and differential interference contrast. Used for demanding multimodal imaging.

Proper citation: Zeiss Axio Observer 3 (RRID:SCR_023693) Copy   

•   Source: SciCrunch Registry

https://industry.nikon.com/en-us/products/x-ray-ct/xt-h-225-xt-h-320/

Provides insight into the inside of components and specimens, allowing users to visualize, analyze and measure complex internal geometry as well as surfaces, all non-destructively. Versatile microfocus CT system for high resolution inspection in R&D and failure analysis of components ranging from tiny plastic connectors up to aluminum castings.

Proper citation: Nikon XT H 225 ST uCT system (RRID:SCR_023687) Copy   

•   Source: SciCrunch Registry

https://www.shoprainin.com/Pipettes/Single-Channel-Manual-Pipettes/Pipet-Lite-XLS-LTS/Pipet-Lite-LTS-Pipette-L-1000XLS%2B/p/17014382

Pipet-Lite™ XLS+ manual single-channel pipette, 100-1000 μL, uses LTS™ LiteTouch™ tips. Accurate and precise, ergonomic, autoclavable liquid end, rugged and durable (L-1000XLS+)

Proper citation: Rainin Pipet-Lite LTS Pipette L-1000XLS+ (RRID:SCR_023894) Copy   

•   Source: SciCrunch Registry

https://www.shoprainin.com/Pipettes/Single-Channel-Manual-Pipettes/Pipet-Lite-XLS-LTS/Pipet-Lite-LTS-Pipette-L-200XLS%2B/p/17014391

Pipet-Lite™ XLS+ manual single-channel pipette, 20-200 μL, uses LTS™ LiteTouch™ tips. Accurate and precise, ergonomic, autoclavable liquid end, rugged and durable (L-200XLS+)

Proper citation: Rainin Pipet-Lite LTS Pipette L-200XLS+ (RRID:SCR_023892) Copy   

•   Source: SciCrunch Registry

https://www.hitachi-keu.com/en-gb/iv/hv-f203gvscl.html

High resolution 1/1.8" 3 CCD Progressive Scan Color Camera with 1600 x 1200 pixel (UXGA) that is installed in the ZEISS Axioscan Z1 slide scanner. Integrated functions include electronic shutter from 1/30s to 1/100000s, Long term Integration up to 4s, AGC, external Trigger, Auto Shading, 6 Colour Masking, Frame-On-Demand Function, White Balance, AGC, Black Level Adjustment and Partial Scan Function.

Proper citation: Hitachi 16-bit 3-CCD color camera HV-F203GV / HV-F203SCL (RRID:SCR_023888) Copy   

•   Source: SciCrunch Registry