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Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pJMD13; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
References:
Comments:
Proper citation: RRID:Addgene_138653 Copy
Species: Other
Genetic Insert: gusA, aac(3)IV, tsr
Vector Backbone Description: Vector Backbone:pSG5; Vector Types:Other, Gene knock out; Bacterial Resistance:Apramycin
References:
Comments: Thermosensitive replication (< 37°C) in Streptomyces, Detection of single/double cross over by X-gluc colour reaction , aac(3)IV
Proper citation: RRID:Addgene_115678 Copy
Species: Other
Genetic Insert: FnCpf1
Vector Backbone Description: Vector Backbone:pKC1139; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
References:
Comments:
Proper citation: RRID:Addgene_129554 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:5613; Vector Backbone:new backbone; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Apramycin
References:
Comments:
Proper citation: RRID:Addgene_126044 Copy
Species: Other
Genetic Insert: Sth1Cas9
Vector Backbone Description: Vector Backbone:pKC1139; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
References:
Comments: Please note that the Addgene verified sequence differs from the depositor's reference sequence. These differences do not affect plasmid function.
Proper citation: RRID:Addgene_129552 Copy
Species: Other
Genetic Insert: SaCas9
Vector Backbone Description: Vector Backbone:pKC1139; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
References:
Comments:
Proper citation: RRID:Addgene_129553 Copy
Species: Synthetic
Genetic Insert: tcp830
Vector Backbone Description: Backbone Size:4460; Vector Backbone:pSET152; Vector Types:Other, Streptomyce integration vector derived from phiC31 int/attP; Bacterial Resistance:Apramycin
References:
Comments: For research using this plasmid, please acknowledge the depositing laboratory and associated citation.
Proper citation: RRID:Addgene_127088 Copy
Species: Synthetic
Genetic Insert: Streptomyces codon optimized spCas9n (D10A)
Vector Backbone Description: Backbone Size:6971; Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
References:
Comments:
Proper citation: RRID:Addgene_131464 Copy
Species: Other
Genetic Insert: streptomyces codon optimized spCas9, sgRNA casette, and ScaligD
Vector Backbone Description: Backbone Size:6971; Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
References:
Comments: Please note that an IS1 transposase sequence was found downstream of Cas9 during Addgene's quality control. The depositor noted that this sequence should NOT affect plasmid function.
Proper citation: RRID:Addgene_125688 Copy
Species: Other
Genetic Insert: streptomyces codon optimized spdCas9, sgRNA casette
Vector Backbone Description: Backbone Size:6971; Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
References:
Comments:
Proper citation: RRID:Addgene_125687 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin
Vector Backbone Description: Backbone Size:3896; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
References:
Comments:
Proper citation: RRID:Addgene_174517 Copy
Species: Other
Genetic Insert: FLP recombinase
Vector Backbone Description: Vector Backbone:pAT03; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
References:
Comments:
Proper citation: RRID:Addgene_154756 Copy
Species: Synthetic
Genetic Insert: XimD and PcPT
Vector Backbone Description: Vector Backbone:pSE152; Vector Types:Synthetic Biology; Bacterial Resistance:Apramycin
References:
Comments:
Proper citation: RRID:Addgene_169109 Copy
Species: Synthetic
Genetic Insert: attL
Vector Backbone Description: Backbone Marker:Bierman M. et al. Gene 1992 Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp.; Backbone Size:3469; Vector Backbone:pOJ260; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
References:
Comments:
Proper citation: RRID:Addgene_172193 Copy
Species: Synthetic
Genetic Insert: D4-TheoR-cas9
Vector Backbone Description: Backbone Size:10994; Vector Backbone:pCRISPomyces-2; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
References:
Comments:
Proper citation: RRID:Addgene_172353 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Jay Keasling lab; Backbone Size:4156; Vector Backbone:pAV-gapdh; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Apramycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.11.16.384693v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_172431 Copy
Species: Other
Genetic Insert: Scocas9
Vector Backbone Description: Vector Backbone:pKC1139; Vector Types:Bacterial Expression, CRISPR, Other, Bacterial Genome Editing; Bacterial Resistance:Apramycin
References:
Comments: More information is provided in the supplemental files. Please note that the sequence of the backbone (pKC1139) was obtained via Sanger sequencing and several regions may have discrepancies due to high GC content. These discrepancies have no functional consequences.
Proper citation: RRID:Addgene_62552 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pKO; Vector Types:Other, Bacterial Knockout Generation; Bacterial Resistance:Apramycin
References:
Comments:
Proper citation: RRID:Addgene_110088 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pACEC3GH; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
References:
Comments:
Proper citation: RRID:Addgene_110085 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:6440; Vector Backbone:pUC19; Vector Types:Other, Expression vector for targeted integration; Bacterial Resistance:Apramycin
References:
Comments: For cloning in homology arms, can use Gateway, restriction ligation, or homology-based cloning such as Gibson/Infusion cloning. eGFP can be swapped for your gene of interest or can be fused (recommend homology base cloning).
Original pSelAct backbone was developed here:
Geize, R. van der, Jong, W. de, Hessels, G. I., Grommen, A. W. F., Jacobs, A. A. C., and Dijkhuizen, L. 2008. A novel method to generate unmarked gene deletions in the intracellular pathogen Rhodococcus equi using 5-fluorocytosine conditional lethality. Nucleic Acids Res. 36:e151
pSelAct-Express was develop and tested here:
Stevens, D. M., Tang, A., and Coaker, G. 2021. A Genetic Toolkit for Investigating Clavibacter Species: Markerless Deletion, Permissive Site Identification, and an Integrative Plasmid. Mol Plant-microbe Interactions. 34:1336–1345
Proper citation: RRID:Addgene_174375 Copy
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