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Species: Synthetic
Genetic Insert: GFP, mCherry
Vector Backbone Description: Vector Backbone:R6Kgamma; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments: This plasmid requires a pir+ strain for growth and transformation. It will be distributed in MFDpir E. coli. MFDpir strain is auxotrophic for DAP (diaminopimelic acid), so it must be added to the media to grow and extract the plasmid (0.3 mM working concentration).
Proper citation: RRID:Addgene_187396 Copy
Species: Synthetic
Genetic Insert: GFPmut3
Vector Backbone Description: Vector Backbone:R6Kgamma; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments: This plasmid requires a pir+ strain for growth and transformation. It will be distributed in MFDpir E. coli. MFDpir strain is auxotrophic for DAP (diaminopimelic acid), so it must be added to the media to grow and extract the plasmid (0.3 mM working concentration).
Proper citation: RRID:Addgene_187381 Copy
Species: Synthetic
Genetic Insert: dGFPmut3_AAV
Vector Backbone Description: Vector Backbone:R6Kgamma; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments: This plasmid requires a pir+ strain for growth and transformation. It will be distributed in MFDpir E. coli. MFDpir strain is auxotrophic for DAP (diaminopimelic acid), so it must be added to the media to grow and extract the plasmid (0.3 mM working concentration).
Proper citation: RRID:Addgene_187388 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:10902; Vector Backbone:pPtPBR1; Vector Types:Other, Gateway Destination vector for protein expression in diatoms; Bacterial Resistance:Other
References:
Comments:
Proper citation: RRID:Addgene_90101 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:11457; Vector Backbone:pPtPBR1; Vector Types:Other, Gateway Destination vector for protein expression in diatoms; Bacterial Resistance:Other
References:
Comments:
Proper citation: RRID:Addgene_90100 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:10591; Vector Backbone:pPtPBR1; Vector Types:Other, Gateway Destination vector for protein expression in diatoms; Bacterial Resistance:Other
References:
Comments:
Proper citation: RRID:Addgene_90099 Copy
Species: Other
Genetic Insert: none
Vector Backbone Description: Vector Backbone:N/A; Vector Types:; Bacterial Resistance:Other
References:
Comments: λDE3 [lacI, lacUV5-T7 gene 1, ind1, sam7, nin5] F- araC14 fhuA31 lacY1 tsx-78 ΔompF480 zcb-222::Tn10 rfbC1 mgl-51 ΔompC178 rpsL136(StrR) xylA5 mtl-1 thiE1
KJ740(DE3) is a λDE3 derivative of the OmpC- & OmpF-deficient strain KJ740 that was described in Ingham C, Buechner M, Adler J, 1990, J Bacteriol 172:3577-3583.
Due to the presence of the lambda (DE3) lysogen, KJ740(DE3) is compatible with expression from the T7 promoter.
The (DE3) lysogen of the parent strain KJ740 was made by Debra T. Hansen using the λDE3 Lysogenization Kit 538 (EMD Millipore #69734-3). The parent strain KJ740 was obtained from the Yale E. coli Genetic Stock Center (CGSC) as strain number 12151.
In contrast to BL21 derivative strains, KJ740(DE3) is not deficient in the proteases Lon and OmpT.
The presence of (DE3) was confirmed by PCR by Felicia M. Craciunescu.
PCR primers that may be used to verify the presence of the (DE3) lysogen:
These primer pairs are expected to generate a PCR product from each of 3 genes within the (DE3) sequence, GenBank accession no. AM946981.2. There are two primer pairs per gene, therefore 6 possible PCR products. The presence of the PCR product of the expected size indicates the presence of the (DE3) lysogen. The PCR reactions will also be run on the negative control non-(DE3) parent strain, KJ740, which is expected to not generate these PCR product sizes.
gene A F1 GACTACATCCGTGAGGTGAATGTGG
gene A R1 GATGACGCAGGCATTATGCTCGCAG
Product size = 645 bp
gene A F2 CCTTTCACATCTGGACAGCGTACAG
gene A R2 GTTGCTGCACCATCCTCTTCCTGC
Product size = 904 bp
gene H F1 GACATCTGGAATCTGCGCAAGGATGA
gene H R1 CTGCAGGATTTTCCCGTCTTTCAGTG
Product size = 291 bp
gene H F2 GTGCGCTGGCGTATGCCTGGTATC
gene H R2 CTGTTCCGTGGCTTCCCGTTCTGC
Product size = 1388 bp
gene J F1 CTTTACCTTCGGTGTACAGGCACTGGTG
gene J R1 GTCCTGCACGAACGTCAGCGTCTG
Product size = 748 bp
gene J F2 CAGACAGGTTGAAACCAGCACGCGTTATC
gene J R2 GTTTGCCTTCCTCCGTGTCCTCCATG
Product size = 407 bp
Please visit https://www.biorxiv.org/content/10.1101/2021.08.26.457821v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_179486 Copy
Species: Synthetic
Genetic Insert: bc145
Vector Backbone Description: Vector Backbone:pBCC096; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments: This plasmid can be transformed into E. coli BW29427 supplemented with 50 µg/mL of diaminopimelic acid (DAP) or any Pir1 strain. Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint.
Proper citation: RRID:Addgene_202279 Copy
Species: Synthetic
Genetic Insert: bc182
Vector Backbone Description: Vector Backbone:pBCC106; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments: This plasmid can be transformed into E. coli BW29427 supplemented with 50 µg/mL of diaminopimelic acid (DAP) or any Pir1 strain. Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint.
Proper citation: RRID:Addgene_202274 Copy
Species: Synthetic
Genetic Insert: bc141
Vector Backbone Description: Vector Backbone:pBCC091; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments: This plasmid can be transformed into E. coli BW29427 supplemented with 50 µg/mL of diaminopimelic acid (DAP) or any Pir1 strain. Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint.
Proper citation: RRID:Addgene_202276 Copy
Species: Synthetic
Genetic Insert: bc144
Vector Backbone Description: Vector Backbone:pBCC096; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments: This plasmid can be transformed into E. coli BW29427 supplemented with 50 µg/mL of diaminopimelic acid (DAP) or any Pir1 strain. Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint.
Proper citation: RRID:Addgene_202278 Copy
Species: Synthetic
Genetic Insert: bc142
Vector Backbone Description: Vector Backbone:pBCC091; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments: This plasmid can be transformed into E. coli BW29427 supplemented with 50 µg/mL of diaminopimelic acid (DAP) or any Pir1 strain. Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint.
Proper citation: RRID:Addgene_202277 Copy
Species: Synthetic
Genetic Insert: bc098
Vector Backbone Description: Vector Backbone:pBCC088; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments: This plasmid can be transformed into E. coli BW29427 supplemented with 50 µg/mL of diaminopimelic acid (DAP) or any Pir1 strain. Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint.
Proper citation: RRID:Addgene_202235 Copy
Species: Synthetic
Genetic Insert: bc106
Vector Backbone Description: Vector Backbone:pBCC090; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments: This plasmid can be transformed into E. coli BW29427 supplemented with 50 µg/mL of diaminopimelic acid (DAP) or any Pir1 strain. Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint.
Proper citation: RRID:Addgene_202238 Copy
Species: Synthetic
Genetic Insert: bc100
Vector Backbone Description: Vector Backbone:pBCC088; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments: This plasmid can be transformed into E. coli BW29427 supplemented with 50 µg/mL of diaminopimelic acid (DAP) or any Pir1 strain. Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint.
Proper citation: RRID:Addgene_202237 Copy
Species: Synthetic
Genetic Insert: bc107
Vector Backbone Description: Vector Backbone:pBCC090; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments: This plasmid can be transformed into E. coli BW29427 supplemented with 50 µg/mL of diaminopimelic acid (DAP) or any Pir1 strain. Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint.
Proper citation: RRID:Addgene_202239 Copy
Species: Synthetic
Genetic Insert: bc096
Vector Backbone Description: Vector Backbone:pBCC088; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments: This plasmid can be transformed into E. coli BW29427 supplemented with 50 µg/mL of diaminopimelic acid (DAP) or any Pir1 strain. Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint.
Proper citation: RRID:Addgene_202233 Copy
Species: Synthetic
Genetic Insert: bc135
Vector Backbone Description: Vector Backbone:pBCC086; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments: This plasmid can be transformed into E. coli BW29427 supplemented with 50 µg/mL of diaminopimelic acid (DAP) or any Pir1 strain. Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint.
Proper citation: RRID:Addgene_202247 Copy
Species: Synthetic
Genetic Insert: bc134
Vector Backbone Description: Vector Backbone:pBCC086; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments: This plasmid can be transformed into E. coli BW29427 supplemented with 50 µg/mL of diaminopimelic acid (DAP) or any Pir1 strain. Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint.
Proper citation: RRID:Addgene_202246 Copy
Species: Synthetic
Genetic Insert: bc187
Vector Backbone Description: Vector Backbone:pBCC087; Vector Types:Bacterial Expression; Bacterial Resistance:Other
References:
Comments: This plasmid can be transformed into E. coli BW29427 supplemented with 50 µg/mL of diaminopimelic acid (DAP) or any Pir1 strain. Please visit https://doi.org/10.1101/2023.04.20.537712 for bioRxiv preprint.
Proper citation: RRID:Addgene_202248 Copy
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