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Plasmids are provided by Addgene and DGRC.

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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pBbdCas9H(-10TC)_arr2
 
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RRID:Addgene_149587 Hygromycin PMID:33257311 Backbone Marker:CJW lab; Vector Backbone:pBbdCas9H(-10TC); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin 2022-04-22 03:27:54 0
pRD393
 
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RRID:Addgene_168774 mEos3.2_H-NSdbd Synthetic Hygromycin PMID:34734265 Backbone Size:5625; Vector Backbone:pFPV27; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2022-04-22 03:32:07 0
pRD445
 
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RRID:Addgene_168775 mEos3.2 Synthetic Hygromycin PMID:34734265 Backbone Size:5625; Vector Backbone:pFPV27; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2022-04-22 03:32:07 0
pGMEH-10M1-clpX-K127R
 
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RRID:Addgene_27374 tetR(sB), clpXtb-K127R Hygromycin PMID:19174563 Backbone Marker:N/A; Backbone Size:6181; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression, Other, Gateway Destination Vector; Bacterial Resistance:Hygromycin changed Lysine 127 to Arginine 2022-04-22 03:37:21 0
pSt-KO
 
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RRID:Addgene_44566 Hygromycin PMID:23315736 Backbone Size:4203; Vector Backbone:pVV16, pGOAL17; Vector Types:Bacterial Expression, Other, E.coli-mycobacteria shuttle vector; Bacterial Resistance:Hygromycin 2022-04-22 03:41:13 0
pNAHA
 
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RRID:Addgene_44169 hemagglutinin gene Other Hygromycin PMID:23319179 Please note that a T55A mutation was identified in the N1 sequence. Backbone Marker:InvivoGen; Backbone Size:6321; Vector Backbone:pVITRO2-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin 2022-04-22 03:41:08 0
pMyC
 
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RRID:Addgene_42192 Hygromycin PMID:33006405 Vector constructed by Dr. Arie Geerlof at EMBL-Hamburg (now at Helmholtz Zentrum München) Backbone Marker:Daugelat et al 2003; Backbone Size:6900; Vector Backbone:pSD26; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2022-04-22 03:40:37 0
pTE-mcs
 
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RRID:Addgene_20320 multiple cloning site Hygromycin PMID:19174563 Backbone Size:5059; Vector Backbone:pMS2; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2022-04-22 03:35:39 0
pTE-10M0X
 
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RRID:Addgene_20318 Pimyc-tetR#10 synthetic gene derived from Tn10 tetR Hygromycin PMID:19174563 There are minor discrepancies between depositor's and Addgene's sequence. Depositor is aware of these and thinks they should not have any affect on function. Backbone Size:5133; Vector Backbone:pTE-mcs; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2022-04-22 03:35:39 0
pCHERRY8
 
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RRID:Addgene_24663 mCherry Synthetic gene Hygromycin PMID:20352111 Backbone Size:5582; Vector Backbone:pSMT3-rpsA; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2022-04-22 03:36:45 0
pCHERRY10
 
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RRID:Addgene_24664 mCherry Synthetic gene Hygromycin PMID:20352111 Backbone Size:5582; Vector Backbone:pSMT3; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2022-04-22 03:36:45 0
pKW08-Lx-Int
 
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1+ mentions
RRID:Addgene_25015 pKW08-Lx-Int Hygromycin PMID:20434484 Backbone Size:8535; Vector Backbone:pMC1M; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2022-04-22 03:36:52 1
pTEC22
 
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RRID:Addgene_30181 Mycobacterium Strong Promoter (MSP) M. marinum Hygromycin PMID:22840407 This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with tdKatuschka2. The Katuschka tandem-dimer ORF is present at bp# 44-1522 Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Other, Mycobacteria expression; Bacterial Resistance:Hygromycin 2022-04-22 03:38:00 0
pTEC21
 
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RRID:Addgene_30180 Mycobacterium Strong Promoter (MSP) M. marinum Hygromycin PMID: This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with Kaede. The Kaede ORF is present at bp# 5624-6304. Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Other, Mycobacteria expression; Bacterial Resistance:Hygromycin 2022-04-22 03:38:00 0
pTEC20
 
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1+ mentions
RRID:Addgene_30179 Mycobacterium Strong Promoter (MSP) M. marinum Hygromycin PMID:23680983 This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with mKate2. The mKate2 ORF is present at bp# 105-803. Backbone Marker:Ramakrishnan et al., 2000; Backbone Size:4981; Vector Backbone:pFPV27; Vector Types:Other, Mycobacteria expression; Bacterial Resistance:Hygromycin 2022-04-22 03:38:00 1
pALACE
 
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RRID:Addgene_31853 Hygromycin PMID:12754249 Plasmid can also be grown in mycobacteria. Also see PMID 15342574 for additional reference. Please note that Addgene were unable to verify the His tag in this construct. In addition there is a 1bp mismatch in our ACEpro-F sequence with the available NCBI reference. Depositing lab believes the construct is functional. Backbone Marker:Koen De Smet Imperial College Medical School; Backbone Size:8221; Vector Backbone:pACE; Vector Types:Bacterial Expression, Other, Escherichia coli-Mycobacterium shuttle plasmid; Bacterial Resistance:Hygromycin 2022-04-22 03:38:19 0
pMN252
 
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RRID:Addgene_32177 rpsL, FRT-hyg-FRT Hygromycin PMID:15563844 This is a suicide vector for gene deletions in mycobacteria; requires rpsL mutant (streptomycin resistant) Vector Backbone:pMN250; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2022-04-22 03:38:22 0
pMycVec2
 
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RRID:Addgene_32361 pJAZ38 ori Hygromycin PMID:11707328 This is a low copy number (one?) plasmid that co-replicates with pAL5000-derived plasmids in mycobacteria. Vector Backbone:pMS2; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2022-04-22 03:38:24 0
pDB88
 
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RRID:Addgene_26958 e. coli galactokinase Escherichia coli Hygromycin PMID:20851171 Backbone Size:10000; Vector Backbone:PMSG346; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin cloned under the MOP promoter 2022-04-22 03:37:14 0
pGMEH-P38-luxAB2
 
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RRID:Addgene_27061 P#38-luxAB synthetic gene Hygromycin PMID:21075796 Backbone Size:7843; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2022-04-22 03:37:16 0

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