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Charité Berlin University of Medicine Transgenic Technologies Core Facility (RRID:SCR_027960)Copy Citation Copied
URL: https://experimentelle-medizin.charite.de/en/transgenic_technologies
Proper Citation: Charité Berlin University of Medicine Transgenic Technologies Core Facility (RRID:SCR_027960)
Description: Core provides services supporting generation, modification, cryopreservation, and rederivation of genetically modified rodent models for biomedical research, generates genetically modified mouse models via both targeted and random mutagenesis using tools such as CRISPR/Cas9 in zygotes or embryonic stem (ES) cells, provides cryopreservation and rederivation of existing animal models to preserve hygienic integrity. Services for rodent transgenic model development include: Pronuclear Injection and amp; Viral Transgenesis with introduction of genetic constructs into zygotes using microinjection or lentiviral systems; ES Cell-Based Techniques: use of embryonic stem cell manipulation for genetic targeting; Embryo Transfer: transferring manipulated embryos into recipient females; In Vitro Fertilization (IVF): assisted reproductive technology to generate embryos for manipulation or cryopreservation; Cryopreservation: freezing of embryos and sperm for archiving or rederivation of lines as required. Facility’s expertise includes quality control of cryopreserved material to ensure viability upon thawing.
Synonyms: Transgenic Technologies Charité-Universitätsmedizin Berlin
Resource Type: service resource, access service resource, core facility
Keywords: ABRF, genetically modified rodent models, generate genetically modified mouse models, cryopreservation and rederivation of existing animal models,
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