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URL: http://www.addgene.org/102562
Proper Citation: RRID:Addgene_102562
Insert Name: Homo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1
Organism: Homo sapiens
Bacterial Resistance: Kanamycin
Defining Citation: PMID:27671650
Vector Backbone Description: Backbone Marker:Novagen.EMD Millipore; Backbone Size:3719; Vector Backbone:pCOLADuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: This plasmid was specifically designed to allow high levels of RAD51 expression in E.coli along with the ability to incorporate non-natural amino acids using amber suppressor technology. It was used to incorporate an artificial amino acid to mimic tyrosine phosphorylation (published in Subramanyam S, et. al. (2016). Proceedings of the National Academy of Sciences 113(41):E6045-E6054).
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Source: Addgene
