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https://edspace.american.edu/openbehavior/project/pycontrol/
Portal provides system that combines open source hardware and software for control of behavioral experiments. Open source framework, combines Python scripts and Micropython microcontroller. Framework can be run through command line interface, or in user friendly graphical user interface that allows users to manage variety of devices such as nose pokes, LED drivers, stepper motor controllers and more. Data collected using this system can then be imported into Python for data analysis.
Proper citation: pyControl project (RRID:SCR_021574) Copy
https://edspace.american.edu/openbehavior/project/photometrybox/
Portal provides open source control system for in vivo fluorescence measurements from deep brain structures developed by University of California, San Francisco scientists. Microcontroller based solution for controlling optical components in intracranial photometry system and processing resulting signal.
Proper citation: PhotometryBox (RRID:SCR_021571) Copy
https://edspace.american.edu/openbehavior/project/hope/
Portal provides brain implant developed by MIT scientists. Implant carries 16 movable tetrodes along with two optic fibers or two injection cannulas. Designed with help of 3D CAD software, allowing modifications for various experimental needs, such as targeting different brain regions and use in other animal species (e.g., rats and non-human primates). Implant can be designed to shape of skull of individual monkeys based on pre-implantation magnetic resonance imaging, thus optimizing fit with skull surface and making recording from deep brain regions more reliable.
Proper citation: Hybrid-drive combining Optogenetics, Pharmacology, and Electrophysiology project (RRID:SCR_021572) Copy
https://edspace.american.edu/openbehavior/project/cerebralux/
Portal provides low cost, open source, wireless probe for optogenetic stimulation. This wireless system for optogenetic stimulation was developed by UCLA scientists. Device consists of two parts. Optical component is mounted on head permanently, whereas electronic component is removable and is applied for each experiment. Device is controlled via custom GUI (built with the TkInter Python 2.7 library) which sends pulses to device via Arduino Uno.
Proper citation: CerebraLux (RRID:SCR_021570) Copy
https://edspace.american.edu/openbehavior/project/nose-poke-device/
Portal provides arduino based, 3D-printed nose poke device for rats. Includes set of instructions on how to build this device using arduino, some 3D printed parts and some off-the-shelf electronic components.
Proper citation: Nose Poke Device (RRID:SCR_021568) Copy
https://edspace.american.edu/openbehavior/project/syringe-pump/
Open source syringe pump apparatus developed by scientists from Michigan Technological University. Syringe pump is designed using freely available open source computer aided design (CAD) software and manufactured using open source RepRap 3-D printer and readily available parts.
Proper citation: Pearce Lab Syringe Pump project (RRID:SCR_021566) Copy
https://edspace.american.edu/openbehavior/project/operant-box-auditory-tasks-obat/
Portal provides operant box designed to train small primates in auditory tasks.Device is based on computer and Arduino Mega 2560 board, named OBAT (Operant Box for Auditory Tasks), designed to present two different auditory stimuli to small primates.All schematics and software source code are available.
Proper citation: Operant Box for Auditory Tasks project (RRID:SCR_021564) Copy
https://edspace.american.edu/openbehavior/project/robucket/
Portal for open source operant chamber, based on Arduino microcontroller platform, that can be used to train mice to respond for reward. Designed by NIDDK scientists.Apparatus contains two nose pokes, drinking well, and solenoid controlled sucrose delivery system. Chamber can run magazine training, fixed ratio and progressive ratio training schedules, and can be programmed to run more complicated behavioral paradigms.
Proper citation: Rodent Operant Bucket project (RRID:SCR_021565) Copy
https://www.nanostring.com/products/geomx-digital-spatial-profiler/geomx-dsp-overview/
Integrated commercial system comprising hardware, software and nCounter chemistry that enables simultaneous, highly multiplex spatial profiling of proteins or RNA in FFPE tissues. DSP platform quantifies abundance of protein or RNA by counting unique indexing oligonucleotides assigned to each target of interest. Used to rapidly and quantitatively assess biological implications of heterogeneity within tissue samples.
Proper citation: NanoString GeoMx Digital Spatial Profiler (RRID:SCR_021660) Copy
https://meyerinst.com/library/NanoZoomer_RS_Brochure_Catalog.pdf
High speed, high resolution digital scanner with network features. User friendly full slide scanner designed to process up to 210 slides automatically.
Proper citation: NanoZoomer 2.0-HT (RRID:SCR_021658) Copy
System includes automated cell counter for trypan blue viability for cell lines and bright field imaging and pattern recognition software to quickly and accurately identify and count individual cells. Cell count, concentration, diameter, and % viability are automatically calculated and reported.
Proper citation: Cellometer Auto T4 (RRID:SCR_021656) Copy
https://edspace.american.edu/openbehavior/project/openfeeder/
Project related to studying reward and feeding behavior. Provides open source automatic feeding system that can be customized for food types from seeds to pellets.System integrates plexiglass tubes, Arduino Uno, motor driver, and piezo sensor to reliably deliver accurate amounts of food, and can also be built using 3D printed parts.
Proper citation: OpenFeeder (RRID:SCR_021552) Copy
Project related to auditory behavioral task data collection.Provided high throughput operant conditioning in mouse homecage system was developed by University of Maryland,College Park, scientists.Automated system for training up to hundreds of mice in auditory behavioral task features custom Matlab GUI running on desktop which connects to Raspberry Pi to administer auditory stimuli from USB soundcard and collects auditory data as well as licking data from water spout with commercially available capacitive sensors. Parts list and build instructions are readily available from paper and associated GitHub repository.
Proper citation: ToneBox (RRID:SCR_021548) Copy
https://www.nanostring.com/products/ncounter-analysis-system/ncounter-systems-overview/
System includes hardware and software. Options include nCounter SPRINT,nCounter MAX or nCounter FLEX. Platform used for multiplex analysis of up to 800 RNA, DNA, or protein targets. System can be combined with GeoMx Digital Spatial Profiler (DSP) to enable high-plex, spatially-resolved RNA, and protein quantification.
Proper citation: NanoString nCounter Analysis System (RRID:SCR_021712) Copy
https://www.cellscale.com/products/microtester/
System includes MicroTester G2 device and software. MicroTester G2 is mechanical test device that can be used to test properties of variety of tissues and materials. Main components of device are load application microbeam, actuators, imaging system, and fluid bath/test chamber. MicroTester G2 software is divided into two modules: data collection module and review and analysis module.
Proper citation: MicroTester G2 system (RRID:SCR_022034) Copy
https://www.coolled.com/products/pe-300ultra/
Highly controllable fluorescence microscopy Illumination System which offers broad spectrum LED illumination for imaging most common fluorophores. System offers precise control over wavelength irradiance and shuttering. Used for LED microscope lighting.
Proper citation: CoolLED pE-300ultra (RRID:SCR_021972) Copy
https://www.alveolelab.com/our-products/primo-micropatterning/
Photopatterning device for performing micropatterning, microfabrication and hydrogel polymerization.Part of PRIMO system.
Proper citation: Alveole PRIMO photopatterning module (RRID:SCR_021941) Copy
Horizontal pipette puller to pull and polish microelectrodes from borosilicate capillaries.
Proper citation: DMZ Universal Electrode Puller (RRID:SCR_022056) Copy
https://biomedical-instruments.com/pdf/Narishige%20MF-830.pdf
Powerful microscope mounted to produce microelectrodes for patch clamping.
Proper citation: Narishige MF 830 Microgrinder (RRID:SCR_022055) Copy
https://www.bio-rad.com/en-us/product/t100-thermal-cycler?ID=LGTWGIE8Z
Small compact 96well thermal cycler. PCR system features intuitive touch screen and thermal gradient technology to optimize reaction in single run.
Proper citation: BioRad T100 Thermal Cycler (RRID:SCR_021921) Copy
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