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Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:7373; Vector Backbone:pUC19; Vector Types:Other, Vector for 2-step knockout by homologous recombination; Bacterial Resistance:Apramycin
References:
Comments: Also known as pSelAct. For cloning in homology arms, can use Gateway, restriction-ligation, or homology-based cloning such as Gibson/Infusion cloning. Please cite at minimum paper 1) and a secondary paper that is most appropriate to your system of interest (2 or 3).
1. First development of pSelAct for knockouts in Rhodococcus equi:
Geize, R. van der, Jong, W. de, Hessels, G. I., Grommen, A. W. F., Jacobs, A. A. C., and Dijkhuizen, L. 2008. A novel method to generate unmarked gene deletions in the intracellular pathogen Rhodococcus equi using 5-fluorocytosine conditional lethality. Nucleic Acids Res. 36:e151
2. First development of pSelAct with Gateway cassette for knockouts in Rhodococcus fasicans:
Savory, E. A., Weisberg, A. J., Stevens, D. M., Creason, A. L., Fuller, S. L., Pearce, E. M., and Chang, J. H. 2020. Phytopathogenic Rhodococcus Have Diverse Plasmids With Few Conserved Virulence Functions. Front Microbiol. 11:1022
3. Later referred to as pSelAct-KO after optimization for knockouts in Clavibacter:
Stevens, D. M., Tang, A., and Coaker, G. 2021. A Genetic Toolkit for Investigating Clavibacter Species: Markerless Deletion, Permissive Site Identification, and an Integrative Plasmid. Mol Plant-microbe Interactions. 34:1336–1345
Proper citation: RRID:Addgene_174374 Copy
Species:
Genetic Insert: dCas9
Vector Backbone Description: Backbone Marker:Chunbo Lou; Backbone Size:4985; Vector Backbone:pPAP; Vector Types:CRISPR, Other, Integrative vector for use in Streptomyces; Bacterial Resistance:Apramycin
References:
Comments:
Proper citation: RRID:Addgene_210388 Copy
Species: Other
Genetic Insert: aac
Vector Backbone Description: Backbone Size:2140; Vector Backbone:pSG5; Vector Types:; Bacterial Resistance:Apramycin
References:
Comments:
Proper citation: RRID:Addgene_69615 Copy
Species: Other
Genetic Insert: aac
Vector Backbone Description: Backbone Size:2140; Vector Backbone:pSG5; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
References:
Comments:
Proper citation: RRID:Addgene_69994 Copy
Species:
Genetic Insert: msfGFP
Vector Backbone Description: Backbone Marker:SEVA collection; Backbone Size:3228; Vector Backbone:pSEVA831; Vector Types:; Bacterial Resistance:Apramycin
References:
Comments: Please visit https://doi.org/10.1101/2023.06.29.547033 for bioRxiv preprint.
Proper citation: RRID:Addgene_208016 Copy
Species: Synthetic
Genetic Insert: AcCas12n
Vector Backbone Description: Vector Backbone:p15a; Vector Types:CRISPR; Bacterial Resistance:Apramycin
References:
Comments:
Proper citation: RRID:Addgene_203806 Copy
Species: Other
Genetic Insert: AsCas12f1
Vector Backbone Description: Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
References:
Comments:
Proper citation: RRID:Addgene_171610 Copy
Species: Synthetic
Genetic Insert: T7RNAP Circuit
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
References:
Comments:
Proper citation: RRID:Addgene_199993 Copy
Species: Synthetic
Genetic Insert: Codon optimized APOBEC1-nCas9-UGI fusion protein
Vector Backbone Description: Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
References:
Comments:
Proper citation: RRID:Addgene_209447 Copy
Species: Synthetic
Genetic Insert: Codon optimized APOBEC1-nCas9-UGI fusion protein
Vector Backbone Description: Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin
References:
Comments:
Proper citation: RRID:Addgene_209446 Copy
Species:
Genetic Insert: Codon Optimized SpCas9
Vector Backbone Description: Vector Backbone:pCM2; Vector Types:CRISPR; Bacterial Resistance:Apramycin
References:
Comments:
Proper citation: RRID:Addgene_217648 Copy
Species: Other
Genetic Insert: scbe3-NG-HF1
Vector Backbone Description: Backbone Marker:Jian Wang; Backbone Size:8525; Vector Backbone:pSCBE3; Vector Types:Bacterial Expression, CRISPR, Other, sgRNA transcription; Bacterial Resistance:Apramycin
References:
Comments: Another insert : a programmable sgRNA cloning cassette
Proper citation: RRID:Addgene_218160 Copy
Species: Other
Genetic Insert: escbe3-NG
Vector Backbone Description: Backbone Marker:Jian Wang; Backbone Size:8525; Vector Backbone:pSCBE3; Vector Types:Bacterial Expression, CRISPR, Other, sgRNA transcription; Bacterial Resistance:Apramycin
References:
Comments: Another insert : a programmable sgRNA cloning cassette
Proper citation: RRID:Addgene_218163 Copy
Species: Other
Genetic Insert: scbe3-HF1
Vector Backbone Description: Backbone Marker:Jian Wang; Backbone Size:8525; Vector Backbone:pSCBE3; Vector Types:Bacterial Expression, CRISPR, Other, sgRNA transcription; Bacterial Resistance:Apramycin
References:
Comments: Another insert : a programmable sgRNA cloning cassette
Proper citation: RRID:Addgene_218156 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:R6Kγ; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
References:
Comments: Tn7 expression vector.
Proper citation: RRID:Addgene_222354 Copy
Species:
Genetic Insert: apramycin resistance gene
Vector Backbone Description: Vector Backbone:pSGAb; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
References:
Comments:
Proper citation: RRID:Addgene_226171 Copy
Species: Other
Genetic Insert: SnaA
Vector Backbone Description: Vector Backbone:pOSV801; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
References:
Comments: encodes biosynthetic genes for threopeptin, including SnaA, SnaB, SnaC, SnaD, SnaE, SnaO, SnaT1, SnaT2
Proper citation: RRID:Addgene_225055 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pMMB207; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
References:
Comments: Gebhardt lab ID # pMJG599.
Proper citation: RRID:Addgene_218361 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pMMB207; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
References:
Comments: Gebhardt lab ID # pMJG588.
Proper citation: RRID:Addgene_218359 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pMMB207; Vector Types:Bacterial Expression; Bacterial Resistance:Apramycin
References:
Comments: Gebhardt lab ID # pMJG120.
Proper citation: RRID:Addgene_218357 Copy
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