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Species: Other
Genetic Insert: GGPP synthase
Vector Backbone Description: Backbone Size:4846; Vector Backbone:pLM304; Vector Types:Yeast Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Addgene NGS sequencing found the following mutations compared to the provided reference sequence: crtE with M176I, K346R, I347M, E353D mutations, crtI with L107F, H427Q mutations, and crtYB with A190T, E307K mutations. tHMG1 contains only residues 531-1054 compared to the NCBI reference seq [M22002.1]. These mutations/truncation do not affect plasmid function.
Proper citation: RRID:Addgene_100539 Copy
Species: Other
Genetic Insert: Floxed TgDHFR-TS*
Vector Backbone Description: Backbone Size:2678; Vector Backbone:pUC19; Vector Types:Other, Toxoplasma expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_100605 Copy
Species: Other
Genetic Insert: N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9
Vector Backbone Description: Backbone Size:7064; Vector Backbone:pEF1a-FB-puro; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_100547 Copy
Species: Other
Genetic Insert: antiGFP nanobody enhancer
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5443; Vector Backbone:pet21a (+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Reference to anti-GFP nanobody enhancer:
Kirchhofer A, Helma J, Schmidthals K, Frauer C, Cui S, Karcher A, Pellis M, Muyldermans S, Casas-Delucchi CS, Cardoso MC, Leonhardt H, Hopfner KP, Rothbauer U. Modulation of protein properties in living cells using nanobodies. Nat Struct Mol Biol. 2010 Jan;17(1):133-8. doi: 10.1038/nsmb.1727. Epub 2009 Dec 13. PMID: 20010839.
Proper citation: RRID:Addgene_192788 Copy
Species: Other
Genetic Insert: Opa1
Vector Backbone Description: Vector Backbone:Gateway destination vector; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_191961 Copy
Species: Other
Genetic Insert: Opa1
Vector Backbone Description: Vector Backbone:pSIlencer; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_191949 Copy
Species: Other
Genetic Insert: 8-hydroxygeraniol oxidoreductase; 10-hydroxygeraniol oxidoreductase; alcohol dehydrogenase 10
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: Genbank accession: KF302069, the CDS sequence has been domesticated (all endogenous BpiI, BsaI, BsmBI and SapI recognition sites removed) and are flanked by an inverted pair of BsaI recognition sites that produce overhangs compatible with the phytobrick assembly standard
Proper citation: RRID:Addgene_177023 Copy
Species: Other
Genetic Insert: iridoid oxidase; CYP76A26
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: Genbank accession: KF302066, amplified from cDNA, contains T359S relative to KF302066, the CDS sequence has been domesticated (all endogenous BpiI, BsaI, BsmBI and SapI recognition sites removed) and are flanked by an inverted pair of BsaI recognition sites that produce overhangs compatible with the phytobrick assembly standard
Proper citation: RRID:Addgene_177026 Copy
Species: Other
Genetic Insert: major latex protein-like
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: Genbank accession: MT108267.1, the CDS sequence has been domesticated (all endogenous BpiI, BsaI, BsmBI and SapI recognition sites removed) and are flanked by an inverted pair of BsaI recognition sites that produce overhangs compatible with the phytobrick assembly standard
Proper citation: RRID:Addgene_177025 Copy
Species: Other
Genetic Insert: 7-deoxyloganetic acid glucosyl transferase; UGT709C2
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: Genbank accession: KF302068, the CDS sequence has been domesticated (all endogenous BpiI, BsaI, BsmBI and SapI recognition sites removed) and are flanked by an inverted pair of BsaI recognition sites that produce overhangs compatible with the phytobrick assembly standard
Proper citation: RRID:Addgene_177027 Copy
Species: Other
Genetic Insert: geranyl pyrophosphate synthase; geranyl diphosphate synthase
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: Genbank accession: GQ369788, transit peptide removed, the CDS sequence has been domesticated (all endogenous BpiI, BsaI, BsmBI and SapI recognition sites removed) and are flanked by an inverted pair of BsaI recognition sites that produce overhangs compatible with the phytobrick assembly standard
Proper citation: RRID:Addgene_177020 Copy
Species: Other
Genetic Insert: geraniol 8-oxidase; geraniol-10-hydroxylase; CYP76B6
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: Genbank accession: AJ251269, the CDS sequence has been domesticated (all endogenous BpiI, BsaI, BsmBI and SapI recognition sites removed) and are flanked by an inverted pair of BsaI recognition sites that produce overhangs compatible with the phytobrick assembly standard
Proper citation: RRID:Addgene_177022 Copy
Species: Other
Genetic Insert: geraniol synthase
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: Genbank accession: JN882024, transit peptide removed, the CDS sequence has been domesticated (all endogenous BpiI, BsaI, BsmBI and SapI recognition sites removed) and are flanked by an inverted pair of BsaI recognition sites that produce overhangs compatible with the phytobrick assembly standard
Proper citation: RRID:Addgene_177021 Copy
Species: Other
Genetic Insert: phiC31 integrase plus SV40 nuclear localization sequence (PKKKRKV)
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: codon optimized for N. benthamiana expression, the CDS sequence has been domesticated (all endogenous BpiI, BsaI, BsmBI and SapI recognition sites removed) and are flanked by an inverted pair of BsaI recognition sites that produce overhangs compatible with the phytobrick assembly standard, derived from GB1496
Proper citation: RRID:Addgene_177035 Copy
Species: Other
Genetic Insert: 2x opattB binding site plus minimal CaMV 35S promoter plus partial TMV 'UTR
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: the CDS sequence has been domesticated (all endogenous BpiI, BsaI, BsmBI and SapI recognition sites removed) and are flanked by an inverted pair of BsaI recognition sites that produce overhangs compatible with the phytobrick assembly standard
Proper citation: RRID:Addgene_177037 Copy
Species: Other
Genetic Insert: secologanin synthase; CYP72C
Vector Backbone Description: Vector Backbone:pTwist; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments: Genbank accession: KF309242.1 or KF415117.1, the CDS sequence has been domesticated (all endogenous BpiI, BsaI, BsmBI and SapI recognition sites removed) and are flanked by an inverted pair of BsaI recognition sites that produce overhangs compatible with the phytobrick assembly standard
Proper citation: RRID:Addgene_177030 Copy
Species: Other
Genetic Insert: 8-hydroxygeraniol oxidoreductase (CrGOR) from Catharanthus roseus
Vector Backbone Description: Vector Backbone:pICH47772 ; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177047 Copy
Species: Other
Genetic Insert: geranyl pyrophosphate synthase (PaGPPS1) from Picea abies
Vector Backbone Description: Vector Backbone:pICH47742 ; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177042 Copy
Species: Other
Genetic Insert: geranyl pyrophosphate synthase (PaGPPS1) from Picea abies
Vector Backbone Description: Vector Backbone:pICH47742 ; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177041 Copy
Species: Other
Genetic Insert: geraniol synthase (CrGES) from Catharanthus roseus
Vector Backbone Description: Vector Backbone:pICH47751 ; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177044 Copy
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