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Species: Drosophila melanogaster
Genetic Insert: mini-white
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments: pUASTP2.1 contains a P-element target for RMCE, the mini-white gene flanked by inverted attP sites. It is a derivative of PUASTP2 in which the UAS and TATA sequences flanking the cassette have been removed. (Specifically, PUASTP2 was digested with BamHI and StuI to remove UAS-TATA-attP, and an attP-containing BamHI-EcoRV fragment from pTA-attP was inserted). This vector can be used to generate target lines by standard P element transgenesis.
Proper citation: RRID:Addgene_13842 Copy
Species: Drosophila melanogaster
Genetic Insert: mini-white
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments: pUASTP2 contains a P-element target for RMCE, the mini-white gene flanked by inverted attP sites. This target cassette was contructed in the parent vector pUAST. This vector can be used to generate target lines by standard P element transgenesis.
Please note that the UAS binding sites and hsp70 promoter of pUAST are outside of the exchange cassette in this vector - i.e. following RMCE, these elements will remain flanking the cassette.
Proper citation: RRID:Addgene_13843 Copy
Species: Homo sapiens
Genetic Insert: NEDD4-2 WW2 domain and human phosphopeptides fused to split mCherry
Vector Backbone Description: Vector Backbone:pNAS derivative; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_111708 Copy
Species: Homo sapiens
Genetic Insert: 14-3-3σ and human phosphopeptides fused to split mCherry
Vector Backbone Description: Vector Backbone:pNAS derivative; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_111706 Copy
Species: Other
Genetic Insert: dCas9N-IntN
Vector Backbone Description: Backbone Size:4670; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_165450 Copy
Species: Other
Genetic Insert: CAHS8
Vector Backbone Description: Backbone Marker:Modified from pET-22b(+) [Park J-M, et al (2002) Biochem Biophys Res Commun 291, 23-28.]; Backbone Size:6128; Vector Backbone:pEThT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_192467 Copy
Species: Other
Genetic Insert: CAHS3
Vector Backbone Description: Backbone Marker:Modified from pET-22b(+) [Park J-M, et al (2002) Biochem Biophys Res Commun 291, 23-28.]; Backbone Size:6128; Vector Backbone:pEThT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_192466 Copy
Species: Homo sapiens
Genetic Insert: Barcoded piggyBac Puro SRT
Vector Backbone Description: Vector Backbone:PB-SRT-Puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_193157 Copy
Species: Other
Genetic Insert: IntC-dCas9C-VPR
Vector Backbone Description: Backbone Size:3530; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_166692 Copy
Species: Homo sapiens
Genetic Insert: Barcoded piggyBac Puro SRT
Vector Backbone Description: Vector Backbone:PB-SRT-Puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_193154 Copy
Species: Homo sapiens
Genetic Insert: Barcoded piggyBac Puro SRT
Vector Backbone Description: Vector Backbone:PB-SRT-Puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_193151 Copy
Species: Other
Genetic Insert: CAHS3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4650; Vector Backbone:pAcGFP1-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_192460 Copy
Species: Drosophila melanogaster
Genetic Insert: Asunder
Vector Backbone Description: Vector Backbone:CS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_47041 Copy
Species: Rattus norvegicus
Genetic Insert: Vamp3
Vector Backbone Description: Backbone Marker:Zuchero lab; Backbone Size:4561; Vector Backbone:pBZ-167 AAV CMV promoter base vector; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments: Vamp3-pHluorin was cloned from a construct kindly provided by Prof. Stephanie Gupton, from this paper: PMID: 29351997.
Please visit https://www.biorxiv.org/content/10.1101/2022.07.08.498895v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_190152 Copy
Species: Mus musculus
Genetic Insert: Vamp2
Vector Backbone Description: Backbone Marker:Zuchero lab; Vector Backbone:pBZ-167 AAV CMV promoter base vector; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments: Vamp2-pHluorin was cloned from a construct kindly provided by Prof. Stephanie Gupton, from this paper: PMID: 29351997.
Please visit https://www.biorxiv.org/content/10.1101/2022.07.08.498895v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_190151 Copy
Species:
Genetic Insert: N-terminal 10His tag
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pBAcPAK8; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_161806 Copy
Species:
Genetic Insert: N-terminal HA tag
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pBAcPAK8; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_161800 Copy
Species: Homo sapiens
Genetic Insert: Barcoded piggyBac tdTomato SRT
Vector Backbone Description: Vector Backbone:AAV-PB-SRT-tdTomato; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_193168 Copy
Species: Homo sapiens
Genetic Insert: Barcoded piggyBac Puro SRT
Vector Backbone Description: Vector Backbone:PB-SRT-Puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_193165 Copy
Species:
Genetic Insert: C-terminal Flag tag
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pBAcPAK8; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_161799 Copy
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