Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
||||
|---|---|---|---|---|---|---|---|---|---|---|
|
pJSC090 - Bacterial expression plasmid for SpCas9 + K855A variant Resource Report Resource Website |
RRID:Addgene_101213 | SpCas9 variant K855A | Other | Ampicillin | PMID:28931002 | Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint. | Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | K855A | 2022-04-22 03:15:03 | 0 |
|
pJSC269 - Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant Resource Report Resource Website |
RRID:Addgene_101220 | SpCas9 variant N692A/M694A/Q695A/H698A/Q926A | Other | Ampicillin | PMID:28931002 | Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint. | Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | N692A, M694A, Q695A, H698A and Q926A | 2022-04-22 03:15:03 | 0 |
|
pJSC011 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant Resource Report Resource Website |
RRID:Addgene_101229 | SpCas9 variant C80S/C574S/S355C/S867C/N692A/M694A/Q695A/H698A | Other | Ampicillin | PMID:28931002 | Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint. | Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | C80S, C574S, S355C, S867C, N692A, M694A, Q695A and H698A | 2022-04-22 03:15:03 | 0 |
|
pJSC120 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A, HNH FRET variant Resource Report Resource Website |
RRID:Addgene_101208 | SpCas9 variant C80S/C574S/S355C/S867C/N497A/R661A/Q695A | Other | Ampicillin | PMID:28931002 | Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint. | Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | C80S, C574S, S355C, S867C, N497A, R661A and Q695A | 2022-04-22 03:15:03 | 0 |
|
pJSC111 - Bacterial expression plasmid for SpCas9-HF1 variant Resource Report Resource Website 1+ mentions |
RRID:Addgene_101209 | SpCas9 variant N497A/R661A/Q695A/Q926A | Other | Ampicillin | PMID:28931002 | Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint. | Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | N497A, R661A, Q695A and Q926A | 2022-04-22 03:15:03 | 1 |
|
pJSC057 - Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant Resource Report Resource Website |
RRID:Addgene_101204 | SpCas9 variant C80S/C574S/E60C/D273C/M1–N497,GGS,V713–D1368 | Other | Ampicillin | PMID:28931002 | Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint. | Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | C80S, C574S, E60C, D273C, M1-N497, GGS, V713-D1368 | 2022-04-22 03:15:03 | 0 |
|
pJSC176 - Bacterial expression plasmid for SpCas9 + Q926A variant Resource Report Resource Website |
RRID:Addgene_101206 | SpCas9 variant Q926A | Other | Ampicillin | PMID:28931002 | Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint. | Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Q926A | 2022-04-22 03:15:03 | 0 |
|
pJSC119 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variant Resource Report Resource Website |
RRID:Addgene_101207 | SpCas9 variant N497A/R661A/Q695A | Other | Ampicillin | PMID:28931002 | Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint. | Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | N497A, R661A and Q695A | 2022-04-22 03:15:03 | 0 |
|
pJSC033 - Bacterial expression plasmid for SpCas9, REC2 FRET variant Resource Report Resource Website |
RRID:Addgene_101200 | SpCas9 variant C80S/C574S/E60C/D273C | Other | Ampicillin | PMID:28931002 | Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint. | Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | C80S, C574S, E60C and D273C | 2022-04-22 03:15:03 | 0 |
|
pJSC038 - Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variant Resource Report Resource Website |
RRID:Addgene_101203 | SpCas9 variant C80S/C574S/S355C/S867C/M1–N497,GGS,V713–D1368 | Other | Ampicillin | PMID:28931002 | Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint. | Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | C80S, C574S, S355C, S867C, M1-N497, GGS, V713-D1368 | 2022-04-22 03:15:03 | 0 |
|
pMCP-GPI Control Plasmid Resource Report Resource Website |
RRID:Addgene_101132 | GPI | Other | Ampicillin | PMID: | Originally cloned by Covalys. The GPI only is 87 bp. The size of ER signal sequence + MCP+GPI is 408 bp. The MCP-tag is N-terminal to the GPI sequence. Additional resistance to Kanamycin. For more information on this plasmid, please see https://www.addgene.org/depositor-collections/neb-cell-imaging-tags/ This plasmid is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com. | Backbone Size:5151; Vector Backbone:pMCP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:02 | 0 | |
|
pDD2002 Resource Report Resource Website |
RRID:Addgene_101198 | LANApi driving green luciferase reporter for CBG68 in pCBG68 backbone. | Other | Ampicillin | PMID:22740392 | Backbone Marker:Promega; Backbone Size:5104; Vector Backbone:pCBG68 Basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:02 | 0 | ||
|
pJSC228 - Bacterial expression plasmid for SpCas9 Cluster 2 variant Resource Report Resource Website |
RRID:Addgene_101233 | SpCas9 variant G582A/V583A/E584A/D585A/N588A | Other | Ampicillin | PMID:28931002 | Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint. | Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | G582A, V583A, E584A, D585A and N588A | 2022-04-22 03:15:03 | 0 |
|
pJSC236 - Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant Resource Report Resource Website |
RRID:Addgene_101235 | SpCas9 variant F491A/M495A/T496A/N497A/Q926A | Other | Ampicillin | PMID:28931002 | Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint. | Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | F491A, M495A, T496A, N497A and Q926A | 2022-04-22 03:15:03 | 0 |
|
4PY6 Resource Report Resource Website |
RRID:Addgene_101272 | 4PY6 | Other | Ampicillin | PMID: | N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: PFA0510w:MAC02W-B09:C201930. SGC or PDB link: http://www.rcsb.org/pdb/explore/explore.do?structureId=4PY6 | Backbone Marker:SGC (Addgene Plasmid #26092); Vector Backbone:pET15-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:04 | 0 | |
|
pMflST-o4 Resource Report Resource Website |
RRID:Addgene_101316 | oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA) | Other | Tetracycline | PMID:28115382 | Vector Backbone:colE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline | 2022-04-22 03:15:05 | 0 | ||
|
pMflT-o2 Resource Report Resource Website |
RRID:Addgene_101310 | oriC2 of M. florum strain L1 (dnaA/dnaN intergenic region) | Other | Tetracycline | PMID:28115382 | Vector Backbone:colE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline | 2022-04-22 03:15:04 | 0 | ||
|
pMflT-o3 Resource Report Resource Website |
RRID:Addgene_101311 | oriC3 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, w/o dnaA) | Other | Tetracycline | PMID:28115382 | Vector Backbone:colE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline | 2022-04-22 03:15:04 | 0 | ||
|
pDD2029 Resource Report Resource Website |
RRID:Addgene_101372 | LANApi TATA box mutation in pBluescript-LANApi,K14 (pDD2000). | Other | Ampicillin | PMID:22740392 | Addgene's sequencing found Y108H and G360D mutations in CBG68luc. These mutations are not known to affect plasmid function. | Backbone Marker:Stratagene; Backbone Size:7150; Vector Backbone:pBluescript II (KS+); Vector Types:Unspecified; Bacterial Resistance:Ampicillin | LANApi TATA box mutation in pBluescript-LANApi,K14 (pDD2000). | 2022-04-22 03:15:05 | 0 |
|
pDD2030 Resource Report Resource Website |
RRID:Addgene_101373 | RBPjk binding site B mutation in pBluescript-LANApi,K14 (pDD2000). | Other | Ampicillin | PMID:22740392 | Addgene's sequencing found V466A and R510C mutations in CBG68luc and a V298A mutation in CBRluc. These mutations are not known to affect plasmid function. | Backbone Marker:Stratagene; Backbone Size:7150; Vector Backbone:pBluescript II (KS+); Vector Types:Unspecified; Bacterial Resistance:Ampicillin | RBPjk binding site B mutation in pBluescript-LANApi,K14 (pDD2000). | 2022-04-22 03:15:06 | 0 |
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the NIF Resources search. From here you can search through a compilation of resources used by NIF and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that NIF has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on NIF then you can log in from here to get additional features in NIF such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
If you are logged into NIF you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the facets that you can filter the data by.
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.