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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pJSC090 - Bacterial expression plasmid for SpCas9 + K855A variant
 
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RRID:Addgene_101213 SpCas9 variant K855A Other Ampicillin PMID:28931002 Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint. Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin K855A 2022-04-22 03:15:03 0
pJSC269 - Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant
 
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RRID:Addgene_101220 SpCas9 variant N692A/M694A/Q695A/H698A/Q926A Other Ampicillin PMID:28931002 Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint. Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin N692A, M694A, Q695A, H698A and Q926A 2022-04-22 03:15:03 0
pJSC011 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant
 
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RRID:Addgene_101229 SpCas9 variant C80S/C574S/S355C/S867C/N692A/M694A/Q695A/H698A Other Ampicillin PMID:28931002 Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint. Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin C80S, C574S, S355C, S867C, N692A, M694A, Q695A and H698A 2022-04-22 03:15:03 0
pJSC120 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A, HNH FRET variant
 
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RRID:Addgene_101208 SpCas9 variant C80S/C574S/S355C/S867C/N497A/R661A/Q695A Other Ampicillin PMID:28931002 Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint. Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin C80S, C574S, S355C, S867C, N497A, R661A and Q695A 2022-04-22 03:15:03 0
pJSC111 - Bacterial expression plasmid for SpCas9-HF1 variant
 
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1+ mentions
RRID:Addgene_101209 SpCas9 variant N497A/R661A/Q695A/Q926A Other Ampicillin PMID:28931002 Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint. Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin N497A, R661A, Q695A and Q926A 2022-04-22 03:15:03 1
pJSC057 - Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant
 
Resource Report
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RRID:Addgene_101204 SpCas9 variant C80S/C574S/E60C/D273C/M1–N497,GGS,V713–D1368 Other Ampicillin PMID:28931002 Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint. Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin C80S, C574S, E60C, D273C, M1-N497, GGS, V713-D1368 2022-04-22 03:15:03 0
pJSC176 - Bacterial expression plasmid for SpCas9 + Q926A variant
 
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RRID:Addgene_101206 SpCas9 variant Q926A Other Ampicillin PMID:28931002 Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint. Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Q926A 2022-04-22 03:15:03 0
pJSC119 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variant
 
Resource Report
Resource Website
RRID:Addgene_101207 SpCas9 variant N497A/R661A/Q695A Other Ampicillin PMID:28931002 Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint. Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin N497A, R661A and Q695A 2022-04-22 03:15:03 0
pJSC033 - Bacterial expression plasmid for SpCas9, REC2 FRET variant
 
Resource Report
Resource Website
RRID:Addgene_101200 SpCas9 variant C80S/C574S/E60C/D273C Other Ampicillin PMID:28931002 Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint. Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin C80S, C574S, E60C and D273C 2022-04-22 03:15:03 0
pJSC038 - Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variant
 
Resource Report
Resource Website
RRID:Addgene_101203 SpCas9 variant C80S/C574S/S355C/S867C/M1–N497,GGS,V713–D1368 Other Ampicillin PMID:28931002 Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint. Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin C80S, C574S, S355C, S867C, M1-N497, GGS, V713-D1368 2022-04-22 03:15:03 0
pMCP-GPI Control Plasmid
 
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RRID:Addgene_101132 GPI Other Ampicillin PMID: Originally cloned by Covalys. The GPI only is 87 bp. The size of ER signal sequence + MCP+GPI is 408 bp. The MCP-tag is N-terminal to the GPI sequence. Additional resistance to Kanamycin. For more information on this plasmid, please see https://www.addgene.org/depositor-collections/neb-cell-imaging-tags/ This plasmid is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com. Backbone Size:5151; Vector Backbone:pMCP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:15:02 0
pDD2002
 
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RRID:Addgene_101198 LANApi driving green luciferase reporter for CBG68 in pCBG68 backbone. Other Ampicillin PMID:22740392 Backbone Marker:Promega; Backbone Size:5104; Vector Backbone:pCBG68 Basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin 2022-04-22 03:15:02 0
pJSC228 - Bacterial expression plasmid for SpCas9 Cluster 2 variant
 
Resource Report
Resource Website
RRID:Addgene_101233 SpCas9 variant G582A/V583A/E584A/D585A/N588A Other Ampicillin PMID:28931002 Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint. Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin G582A, V583A, E584A, D585A and N588A 2022-04-22 03:15:03 0
pJSC236 - Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant
 
Resource Report
Resource Website
RRID:Addgene_101235 SpCas9 variant F491A/M495A/T496A/N497A/Q926A Other Ampicillin PMID:28931002 Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint. Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin F491A, M495A, T496A, N497A and Q926A 2022-04-22 03:15:03 0
4PY6
 
Resource Report
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RRID:Addgene_101272 4PY6 Other Ampicillin PMID: N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: PFA0510w:MAC02W-B09:C201930. SGC or PDB link: http://www.rcsb.org/pdb/explore/explore.do?structureId=4PY6 Backbone Marker:SGC (Addgene Plasmid #26092); Vector Backbone:pET15-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:15:04 0
pMflST-o4
 
Resource Report
Resource Website
RRID:Addgene_101316 oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA) Other Tetracycline PMID:28115382 Vector Backbone:colE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline 2022-04-22 03:15:05 0
pMflT-o2
 
Resource Report
Resource Website
RRID:Addgene_101310 oriC2 of M. florum strain L1 (dnaA/dnaN intergenic region) Other Tetracycline PMID:28115382 Vector Backbone:colE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline 2022-04-22 03:15:04 0
pMflT-o3
 
Resource Report
Resource Website
RRID:Addgene_101311 oriC3 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, w/o dnaA) Other Tetracycline PMID:28115382 Vector Backbone:colE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline 2022-04-22 03:15:04 0
pDD2029
 
Resource Report
Resource Website
RRID:Addgene_101372 LANApi TATA box mutation in pBluescript-LANApi,K14 (pDD2000). Other Ampicillin PMID:22740392 Addgene's sequencing found Y108H and G360D mutations in CBG68luc. These mutations are not known to affect plasmid function. Backbone Marker:Stratagene; Backbone Size:7150; Vector Backbone:pBluescript II (KS+); Vector Types:Unspecified; Bacterial Resistance:Ampicillin LANApi TATA box mutation in pBluescript-LANApi,K14 (pDD2000). 2022-04-22 03:15:05 0
pDD2030
 
Resource Report
Resource Website
RRID:Addgene_101373 RBPjk binding site B mutation in pBluescript-LANApi,K14 (pDD2000). Other Ampicillin PMID:22740392 Addgene's sequencing found V466A and R510C mutations in CBG68luc and a V298A mutation in CBRluc. These mutations are not known to affect plasmid function. Backbone Marker:Stratagene; Backbone Size:7150; Vector Backbone:pBluescript II (KS+); Vector Types:Unspecified; Bacterial Resistance:Ampicillin RBPjk binding site B mutation in pBluescript-LANApi,K14 (pDD2000). 2022-04-22 03:15:06 0

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