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Species: Synthetic
Genetic Insert: Avian sarcoma and leukemia virus receptor 950
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5604; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Cre/Lox, Other; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_38044 Copy
Species: Homo sapiens
Genetic Insert: UGGT2
Vector Backbone Description: Backbone Marker:Eric-Paul Bennett (Addgene plasmid # 68369); Backbone Size:2376; Vector Backbone:EPB104; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_106834 Copy
Species: Homo sapiens
Genetic Insert: UGGT1
Vector Backbone Description: Backbone Marker:Eric-Paul Bennett (Addgene plasmid # 68369); Backbone Size:2376; Vector Backbone:EPB104; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_106833 Copy
Species: Mus musculus
Genetic Insert: mouse NFL
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4242; Vector Backbone:pEGFP-C1 with EGFP sequence removed; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Plasmid first described in Yan et al. (2007), "The polypeptide composition of moving and stationary neurofilaments in cultured sympathetic neurons", Cell Motility and the Cytoskeleton 64:299–309.
Mouse NFL cDNA sequence deposited in Genbank (Accession number DQ201635).
Proper citation: RRID:Addgene_83127 Copy
Species: Mus musculus
Genetic Insert: Clathrin Light Chain
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Cloned from IMAGE clone: 3154277 (MGC:68133).
Proper citation: RRID:Addgene_27680 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Roel de Paus; Backbone Size:12878; Vector Backbone:pLZRS-IRES-deltaNGFR; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: Extracellular domain of NGFR is expressed in tandem with the insert, thus allowing FACS selection of cells retrovirally expressing the insert.
Discrepancies between the full and QC sequence should not have any functional consequence.
This plasmid has been found to be somewhat unstable and prone to recombination. You may need to screen multiple colonies to isolate the full-length, monomeric version of this plasmid.
Proper citation: RRID:Addgene_72930 Copy
Species: Danio rerio
Genetic Insert: flk1
Vector Backbone Description: Backbone Size:0; Vector Backbone:na; Vector Types:; Bacterial Resistance:Ampicillin
References:
Comments: For riboprobe, digest with EcoRI and use T7 polymerase.
Proper citation: RRID:Addgene_13061 Copy
Species: Homo sapiens
Genetic Insert: Wnt7A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_35914 Copy
Species: Danio rerio
Genetic Insert: zebrafish ubiquitin promoter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2692; Vector Backbone:pENTR5' TOPO; Vector Types:Other, Multisite Gateway 5' entry vector; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_27320 Copy
Species: Homo sapiens
Genetic Insert: Amyloid-Precursor-Protein
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pcDNA FRT TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_114194 Copy
Species: Homo sapiens
Genetic Insert: STIM1
Vector Backbone Description: Backbone Marker:Invitrogen/Thermo; Backbone Size:2717; Vector Backbone:pENTR1a; Vector Types:Other, Gateway entry clone; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_114176 Copy
Species: Other
Genetic Insert: Peptide: N-glycosidase F
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KS(-); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_114274 Copy
Species: Synthetic
Genetic Insert: 3xNLS_SpCas9
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET-21 a; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid is also associated with the following publication:
PMID 30911135: Highly efficient therapeutic gene editing of human hematopoietic stem cells
Proper citation: RRID:Addgene_114365 Copy
Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5603; Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_114471 Copy
Species: Homo sapiens
Genetic Insert: RXR alpha
Vector Backbone Description: Backbone Marker:pBABE-puro is available at Addgene; Backbone Size:5169; Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_11441 Copy
Species: Other
Genetic Insert: Tol2 transposase
Vector Backbone Description: Backbone Marker:Koichi Kawakami; Vector Backbone:pCAGGS-T2TP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_114725 Copy
Species: Homo sapiens
Genetic Insert: huntingtin, exon 1, 46Q glutamines
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5900; Vector Backbone:pET-32a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_11515 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT5G46330
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47032); Vector Backbone:pECIA2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence: KQSFEPEIEALKSFKNGISNDPLGVLSDWTIIGSLRHCNWTGITCDSTGHVVSVSLLEKQLEGVLSPAIANLTYLQVLDLTSNSFTGKIPAEIGKLTELNQLILYLNYFSGSIPSGIWELKNIFYLDLRNNLLSGDVPEEICKTSSLVLIGFDYNNLTGKIPECLGDLVHLQMFVAAGNHLTGSIPVSIGTLANLTDLDLSGNQLTGKIPRDFGNLLNLQSLVLTENLLEGDIPAEIGNCSSLVQLELYDNQLTGKIPAELGNLVQLQALRIYKNKLTSSIPSSLFRLTQLTHLGLSENHLVGPISEEIGFLESLEVLTLHSNNFTGEFPQSITNLRNLTVLTVGFNNISGELPADLGLLTNLRNLSAHDNLLTGPIPSSISNCTGLKLLDLSHNQMTGEIPRGFGRMNLTFISIGRNHFTGEIPDDIFNCSNLETLSVADNNLTGTLKPLIGKLQKLRILQVSYNSLTGPIPREIGNLKDLNILYLHSNGFTGRIPREMSNLTLLQGLRMYSNDLEGPIPEEMFDMKLLSVLDLSNNKFSGQIPALFSKLESLTYLSLQGNKFNGSIPASLKSLSLLNTFDISDNLLTGTIPGELLASLKNMQLYLNFSNNLLTGTIPKELGKLEMVQEIDLSNNLFSGSIPRSLQACKNVFTLDFSQNNLSGHIPDEVFQGMDMIISLNLSRNSFSGEIPQSFGNMTHLVSLDLSSNNLTGEIPESLANLSTLKHLKLASNNLKGHVPESGVFKNINASDLMGNTDLCGSKKPLKPCTIKQKSSHFSKR
Proper citation: RRID:Addgene_115120 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:8266; Vector Backbone:pDest-560; Vector Types:Bacterial Expression, Other, Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_11518 Copy
Species: Homo sapiens
Genetic Insert: CD81
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4400; Vector Backbone:pCDM8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_11588 Copy
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