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http://www.addgene.org/101910

Species: Other
Genetic Insert: β-glucosidase
Vector Backbone Description: Backbone Size:5711; Vector Backbone:pET16b; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_101910 Copy   

•   Source: Addgene

http://www.addgene.org/101911

Species: Other
Genetic Insert: β-glucosidase
Vector Backbone Description: Backbone Size:5711; Vector Backbone:pET16b; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_101911 Copy   

•   Source: Addgene

http://www.addgene.org/101887

Species: Other
Genetic Insert: PP-BRD3
Vector Backbone Description: Backbone Marker:Cheryl Arrowsmith (Addgene plasmid # 26092); Vector Backbone:pET15-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: LdBPK_091320.1:MAC055-F01:C235018. SGC or PDG link: http://www.thesgc.org/structures/5TCK/

Proper citation: RRID:Addgene_101887 Copy   

•   Source: Addgene

http://www.addgene.org/101888

Species: Other
Genetic Insert: PP-BRD3
Vector Backbone Description: Backbone Marker:Cheryl Arrowsmith (Addgene plasmid # 26092); Vector Backbone:pET15-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: LdBPK_091320.1:MAC055-C02:C235023. SGC or PDG links: http://www.thesgc.org/structures/5TCM/ http://www.thesgc.org/structures/6BYA/

Proper citation: RRID:Addgene_101888 Copy   

•   Source: Addgene

http://www.addgene.org/101882

Species: Other
Genetic Insert: PP-BRD19
Vector Backbone Description: Backbone Marker:Cheryl Arrowsmith (Addgene plasmid # 26092); Vector Backbone:pET15-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: Tb427.10.8150:MAC054-H04:C234720. SGC or PDG link: http://www.thesgc.org/structures/5KO4/

Proper citation: RRID:Addgene_101882 Copy   

•   Source: Addgene

http://www.addgene.org/102355

Species: Other
Genetic Insert: TAL-L1-1.3
Vector Backbone Description: Vector Backbone:pRN3p; Vector Types:Other, T3 in vitro transcription for expression in mammalian cells; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_102355 Copy   

•   Source: Addgene

http://www.addgene.org/102285

Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pCas9-CR4; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
References:
Comments:

Proper citation: RRID:Addgene_102285 Copy   

•   Source: Addgene

http://www.addgene.org/102337

Species: Other
Genetic Insert: RNAP Beta Prime Subunit with C-terminal FLAG TAG
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:; Bacterial Resistance:Kanamycin
References:
Comments:

Proper citation: RRID:Addgene_102337 Copy   

•   Source: Addgene

http://www.addgene.org/102535

Species: Other
Genetic Insert: Minos transposase mRNA
Vector Backbone Description: Vector Backbone:pBluescript SK II+; Vector Types:; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_102535 Copy   

•   Source: Addgene

http://www.addgene.org/102786

Species: Other
Genetic Insert: reverse transcription xenopolymerase with 3'-5' deficient exonuclease
Vector Backbone Description: Vector Backbone:modified pET21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_102786 Copy   

•   Source: Addgene

http://www.addgene.org/102771

Species: Other
Genetic Insert: C1V1::FusionRed::Kv2.1
Vector Backbone Description: Backbone Size:5366; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_102771 Copy   

•   Source: Addgene

http://www.addgene.org/102939

Species: Other
Genetic Insert: mariner transposon flanked by MmeI modified inverted repeats and the himar1C9 transposase
Vector Backbone Description: Vector Backbone:pSAM; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: The Plac promoter from pGFP-Mut3.1 (Clonetech), along with its associated ribosome binding sequence, was amplified via PCR. Engineered 59 BamHI and 39 NdeI restriction sites were used to sub-clone the resulting fragment into a BamHI/NdeI (New England Biolabs) double digested pSAM_Bt vector upstream of the himar1C9 transposase gene. The kanamycin resistance gene from pKD4 was amplified and ligated using the restriction sites MfeI and XbaI, replacing the erythromycin resistance gene ermG in pSAM_Bt. The resulting transposon mutagenesis vector, pSAM-Ec, was stored and propagated in the pir+ E. coli strain EcS17.

Proper citation: RRID:Addgene_102939 Copy   

•   Source: Addgene

http://www.addgene.org/102814

Species: Other
Genetic Insert: pknG
Vector Backbone Description: Backbone Size:5708; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Note: Addgene's quality control sequencing has identified a frameshift mutation near the C-terminus of the pET-15b backbone-resident Lac-I ORF. However, the depositing lab states that this mutation does not affect PknG protein expression.

Proper citation: RRID:Addgene_102814 Copy   

•   Source: Addgene

http://www.addgene.org/102893

Species: Other
Genetic Insert: OCT4-gRNAs-1-5-PGK-puro
Vector Backbone Description: Vector Backbone:PB-GG-PGK-puro; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_102893 Copy   

•   Source: Addgene

http://www.addgene.org/102902

Species: Other
Genetic Insert: OSK2M2L1-gRNAs-PGK-puro
Vector Backbone Description: Vector Backbone:GG-EBNA; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_102902 Copy   

•   Source: Addgene

http://www.addgene.org/102904

Species: Other
Genetic Insert: EEA-guide1-PGK-Puro
Vector Backbone Description: Vector Backbone:GG-EBNA; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_102904 Copy   

•   Source: Addgene

http://www.addgene.org/103001

Species: Other
Genetic Insert: PfSSB_W166C
Vector Backbone Description: Vector Backbone:pET22B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Usage: The expressed protein is a Plasmodium falciparum single-stranded DNA-binding protein, PfSSB, with two point mutations, C93A and W166C, for subsequent labeling at the 166 cysteine with one IDCC (N-[2-(iodoacetamido)ethyl]-7-diethylaminocoumarin-3-carboxamide) or Cy3B per subunit. PfSSB was expressed without the N-terminal apicoplast localisation sequence (76 amino acids) but amino acids are numbered for the full-length protein, that is the first amino acid in the expressed protein is M77.W166C is the equivalent position to W88 in E. coli SSB, which was labeled with Cy3B for single molecule measurements of DNA unwinding using TIRF (Fili, N.; Mashanov, G.; Toseland, C. P.; Batters, C.; Wallace, M. I.; Yeeles, J. T. P.; Dillingham, M. S.; Webb, M. R.; Molloy, J. E., Visualizing DNA unwinding by helicases at the single molecule level. Nucleic Acids Res. 2010, 38, 4448-4457.) Protocol: See Hedgethorne and Webb (2012), which is also followed for PfSSB purification, except with the omission of the PEI precipitation step. Hedgethorne, K.; Webb, M. R., Fluorescent SSB as a reagentless biosensor for single-stranded DNA. Methods Mol. Biol. 2012, 922, 219-233.

Proper citation: RRID:Addgene_103001 Copy   

•   Source: Addgene

http://www.addgene.org/103000

Species: Other
Genetic Insert: PfSSB
Vector Backbone Description: Vector Backbone:pET22B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: The expressed protein is a Plasmodium falciparum single-stranded DNA-binding protein, PfSSB, for subsequent labeling at the single, native cysteine with one IDCC (N-[2-(iodoacetamido)ethyl]-7-diethylaminocoumarin-3-carboxamide) per subunit. This adduct is then used as a fluorescent single-stranded DNA (ssDNA) biosensor, having ~20-fold fluorescence increase on binding ssDNA with very high affinity. PfSSB was expressed without the N-terminal apicoplast localisation sequence (76 amino acids) but amino acids are numbered for the full-length protein, that is the first amino acid in the expressed protein is M77. Protocol See Hedgethorne and Webb (2012), which is also followed for PfSSB purification, except with the omission of the PEI precipitation step. Hedgethorne, K.; Webb, M. R., Fluorescent SSB as a reagentless biosensor for single-stranded DNA. Methods Mol. Biol. 2012, 922, 219-233.

Proper citation: RRID:Addgene_103000 Copy   

•   Source: Addgene

http://www.addgene.org/103055

Species: Other
Genetic Insert: Ebolavirus minigenome
Vector Backbone Description: Vector Backbone:pCAGGs; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_103055 Copy   

•   Source: Addgene

http://www.addgene.org/103052

Species: Other
Genetic Insert: L gene of Ebola virus
Vector Backbone Description: Vector Backbone:pCAGGs; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_103052 Copy   

•   Source: Addgene