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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pET_BGL1 Resource Report Resource Website |
RRID:Addgene_101910 | β-glucosidase | Other | Ampicillin | PMID:29516725 | Backbone Size:5711; Vector Backbone:pET16b; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:12 | 0 | ||
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pET_BGL2 Resource Report Resource Website |
RRID:Addgene_101911 | β-glucosidase | Other | Ampicillin | PMID:29516725 | Backbone Size:5711; Vector Backbone:pET16b; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:12 | 0 | ||
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5TCK (PP-BRD3) Resource Report Resource Website |
RRID:Addgene_101887 | PP-BRD3 | Other | Ampicillin | PMID: | N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: LdBPK_091320.1:MAC055-F01:C235018. SGC or PDG link: http://www.thesgc.org/structures/5TCK/ | Backbone Marker:Cheryl Arrowsmith (Addgene plasmid # 26092); Vector Backbone:pET15-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:11 | 0 | |
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5TCM (PP-BRD3) Resource Report Resource Website |
RRID:Addgene_101888 | PP-BRD3 | Other | Ampicillin | PMID: | N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: LdBPK_091320.1:MAC055-C02:C235023. SGC or PDG links: http://www.thesgc.org/structures/5TCM/ http://www.thesgc.org/structures/6BYA/ | Backbone Marker:Cheryl Arrowsmith (Addgene plasmid # 26092); Vector Backbone:pET15-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:12 | 0 | |
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5KO4 (PP-BRD19) Resource Report Resource Website |
RRID:Addgene_101882 | PP-BRD19 | Other | Ampicillin | PMID: | N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: Tb427.10.8150:MAC054-H04:C234720. SGC or PDG link: http://www.thesgc.org/structures/5KO4/ | Backbone Marker:Cheryl Arrowsmith (Addgene plasmid # 26092); Vector Backbone:pET15-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:11 | 0 | |
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TAL-L1-1.3-Ctrl Resource Report Resource Website |
RRID:Addgene_102355 | TAL-L1-1.3 | Other | Ampicillin | PMID:28846101 | Vector Backbone:pRN3p; Vector Types:Other, T3 in vitro transcription for expression in mammalian cells; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:13 | 0 | ||
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pEM-Cas9noSsrA Resource Report Resource Website |
RRID:Addgene_102285 | Cas9 | Other | Chloramphenicol | PMID:28915012 | Vector Backbone:pCas9-CR4; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol | removed ssrA tag | 2022-04-22 03:15:13 | 0 | |
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RNA polymerase beta prime subunit FLAG Resource Report Resource Website |
RRID:Addgene_102337 | RNAP Beta Prime Subunit with C-terminal FLAG TAG | Other | Kanamycin | PMID:29239721 | Vector Backbone:pBR322; Vector Types:; Bacterial Resistance:Kanamycin | 2022-04-22 03:15:13 | 0 | ||
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pBlueSKMimRNA Resource Report Resource Website |
RRID:Addgene_102535 | Minos transposase mRNA | Other | Ampicillin | PMID:15238525 | Vector Backbone:pBluescript SK II+; Vector Types:; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:15 | 0 | ||
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pET_RTX_(exo-) Resource Report Resource Website |
RRID:Addgene_102786 | reverse transcription xenopolymerase with 3'-5' deficient exonuclease | Other | Ampicillin | PMID:27339990 | Vector Backbone:modified pET21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:19 | 0 | ||
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pAAV-CamKIIa-C1V1::FusionRed::Kv2.1 Resource Report Resource Website |
RRID:Addgene_102771 | C1V1::FusionRed::Kv2.1 | Other | Ampicillin | PMID: | Backbone Size:5366; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:19 | 0 | ||
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pSAM_Ec Resource Report Resource Website |
RRID:Addgene_102939 | mariner transposon flanked by MmeI modified inverted repeats and the himar1C9 transposase | Other | Ampicillin and Kanamycin | PMID:23990803 | The Plac promoter from pGFP-Mut3.1 (Clonetech), along with its associated ribosome binding sequence, was amplified via PCR. Engineered 59 BamHI and 39 NdeI restriction sites were used to sub-clone the resulting fragment into a BamHI/NdeI (New England Biolabs) double digested pSAM_Bt vector upstream of the himar1C9 transposase gene. The kanamycin resistance gene from pKD4 was amplified and ligated using the restriction sites MfeI and XbaI, replacing the erythromycin resistance gene ermG in pSAM_Bt. The resulting transposon mutagenesis vector, pSAM-Ec, was stored and propagated in the pir+ E. coli strain EcS17. | Vector Backbone:pSAM; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin | 2022-04-22 03:15:22 | 0 | |
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pET15b-pknG Resource Report Resource Website |
RRID:Addgene_102814 | pknG | Other | Ampicillin | PMID:15155913 | Note: Addgene's quality control sequencing has identified a frameshift mutation near the C-terminus of the pET-15b backbone-resident Lac-I ORF. However, the depositing lab states that this mutation does not affect PknG protein expression. | Backbone Size:5708; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:20 | 0 | |
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PB-GG-OCT4-1-5-PGK-Puro Resource Report Resource Website |
RRID:Addgene_102893 | OCT4-gRNAs-1-5-PGK-puro | Other | Ampicillin | PMID:29980666 | Vector Backbone:PB-GG-PGK-puro; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:22 | 0 | ||
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GG-EBNA-OSK2M2L1-PP Resource Report Resource Website |
RRID:Addgene_102902 | OSK2M2L1-gRNAs-PGK-puro | Other | Ampicillin | PMID:29980666 | Vector Backbone:GG-EBNA; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:22 | 0 | ||
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GG-EBNA-EEA-guide1-PGK-Puro Resource Report Resource Website |
RRID:Addgene_102904 | EEA-guide1-PGK-Puro | Other | Ampicillin | PMID:29980666 | Vector Backbone:GG-EBNA; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:22 | 0 | ||
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pET22b_PfSSB_W166C Resource Report Resource Website |
RRID:Addgene_103001 | PfSSB_W166C | Other | Ampicillin | PMID:29466468 | Usage: The expressed protein is a Plasmodium falciparum single-stranded DNA-binding protein, PfSSB, with two point mutations, C93A and W166C, for subsequent labeling at the 166 cysteine with one IDCC (N-[2-(iodoacetamido)ethyl]-7-diethylaminocoumarin-3-carboxamide) or Cy3B per subunit. PfSSB was expressed without the N-terminal apicoplast localisation sequence (76 amino acids) but amino acids are numbered for the full-length protein, that is the first amino acid in the expressed protein is M77.W166C is the equivalent position to W88 in E. coli SSB, which was labeled with Cy3B for single molecule measurements of DNA unwinding using TIRF (Fili, N.; Mashanov, G.; Toseland, C. P.; Batters, C.; Wallace, M. I.; Yeeles, J. T. P.; Dillingham, M. S.; Webb, M. R.; Molloy, J. E., Visualizing DNA unwinding by helicases at the single molecule level. Nucleic Acids Res. 2010, 38, 4448-4457.) Protocol: See Hedgethorne and Webb (2012), which is also followed for PfSSB purification, except with the omission of the PEI precipitation step. Hedgethorne, K.; Webb, M. R., Fluorescent SSB as a reagentless biosensor for single-stranded DNA. Methods Mol. Biol. 2012, 922, 219-233. | Vector Backbone:pET22B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | C93A, W166C | 2022-04-22 03:15:24 | 0 |
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pET22b_PfSSB Resource Report Resource Website |
RRID:Addgene_103000 | PfSSB | Other | Ampicillin | PMID:29466468 | The expressed protein is a Plasmodium falciparum single-stranded DNA-binding protein, PfSSB, for subsequent labeling at the single, native cysteine with one IDCC (N-[2-(iodoacetamido)ethyl]-7-diethylaminocoumarin-3-carboxamide) per subunit. This adduct is then used as a fluorescent single-stranded DNA (ssDNA) biosensor, having ~20-fold fluorescence increase on binding ssDNA with very high affinity. PfSSB was expressed without the N-terminal apicoplast localisation sequence (76 amino acids) but amino acids are numbered for the full-length protein, that is the first amino acid in the expressed protein is M77. Protocol See Hedgethorne and Webb (2012), which is also followed for PfSSB purification, except with the omission of the PEI precipitation step. Hedgethorne, K.; Webb, M. R., Fluorescent SSB as a reagentless biosensor for single-stranded DNA. Methods Mol. Biol. 2012, 922, 219-233. | Vector Backbone:pET22B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:24 | 0 | |
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pCAGGS_3E5E_luciferase Resource Report Resource Website |
RRID:Addgene_103055 | Ebolavirus minigenome | Other | Ampicillin | PMID:28807685 | Vector Backbone:pCAGGs; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:24 | 0 | ||
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pCAGGS_L_EBOV Resource Report Resource Website |
RRID:Addgene_103052 | L gene of Ebola virus | Other | Ampicillin | PMID:28807685 | Vector Backbone:pCAGGs; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:24 | 0 |
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