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Species: Homo sapiens
Genetic Insert: SETD8
Vector Backbone Description: Backbone Marker:Cheryl Arrowsmith (Addgene plasmid # 26096); Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: SETD8:PBC001-G01:C233009. SGC or PDG link:
http://www.thesgc.org/structures/5T5G/
http://www.thesgc.org/structures/5TH7/
Proper citation: RRID:Addgene_101886 Copy
Species: Synthetic
Genetic Insert: 7SK paired gRNA insert
Vector Backbone Description: Backbone Size:4762; Vector Backbone:pDONR221; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_102440 Copy
Species: Other
Genetic Insert: RNAP Beta Prime Subunit with C-terminal FLAG TAG
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_102337 Copy
Species:
Genetic Insert: eBFP2
Vector Backbone Description: Backbone Size:1759; Vector Backbone:pJL1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_102640 Copy
Species:
Genetic Insert: Minos transposon carrying TetR gene
Vector Backbone Description: Vector Backbone:Lorist6; Vector Types:; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_102537 Copy
Species: Homo sapiens
Genetic Insert: MYC
Vector Backbone Description: Backbone Size:4319; Vector Backbone:pCMV-Tag-4A; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_102625 Copy
Species:
Genetic Insert: mTagBFP2
Vector Backbone Description: Backbone Size:1759; Vector Backbone:pJL1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_102638 Copy
Species:
Genetic Insert: mTFP1
Vector Backbone Description: Backbone Size:1759; Vector Backbone:pJL1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_102635 Copy
Species: Homo sapiens
Genetic Insert: Homo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1
Vector Backbone Description: Backbone Marker:Novagen.EMD Millipore; Backbone Size:3719; Vector Backbone:pCOLADuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: This plasmid was specifically designed to allow high levels of RAD51 expression in E.coli along with the ability to incorporate non-natural amino acids using amber suppressor technology. It was used to incorporate an artificial amino acid to mimic tyrosine phosphorylation (published in Subramanyam S, et. al. (2016). Proceedings of the National Academy of Sciences 113(41):E6045-E6054).
Proper citation: RRID:Addgene_102562 Copy
Species:
Genetic Insert: J23106:B0034:spisPink:rrnBT1-T7TE
Vector Backbone Description: Backbone Marker:Self-made; Backbone Size:2195; Vector Backbone:derived from pSB1K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_102701 Copy
Species:
Genetic Insert: J23106:B0034:spisPink:rrnBT1-T7TE
Vector Backbone Description: Backbone Marker:Self-made; Backbone Size:2195; Vector Backbone:derived from pSB1K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_102703 Copy
Species: Homo sapiens
Genetic Insert: APPL1
Vector Backbone Description: Backbone Size:4722; Vector Backbone:mCherry-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: *Note: Addgene's quality control sequencing finds additional residues KPNSAVDGTAGPGSTGSR appended to the C-terminus of the APPL1 protein. These additional residues are not thought to affect protein function.
Proper citation: RRID:Addgene_102784 Copy
Species: Homo sapiens
Genetic Insert: Rab5a
Vector Backbone Description: Backbone Size:4731; Vector Backbone:EGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: mCherry-C2-Rab5a encodes wild-type human Rab5a,(NM_004162) as a fusion protein with mCherry. Rab5a was inserted between the Eco R1 and Sal 1 restriction sites. mCherry-C2 encodes a monomeric variant of DsRed. The backbone plasmid is pEGFP-C1 (Clontech). The C2 version of mCherry was created by insertion of a short piece of DNA between Xho 1 and EcoR1 restriction sites. In the resulting multiple cloning site, the Sac 1 restriction site has been eliminated and the Eco R1 restriction site is in frame.
Proper citation: RRID:Addgene_102785 Copy
Species:
Genetic Insert: Linker sequence
Vector Backbone Description: Backbone Marker:Self-made; Backbone Size:2267; Vector Backbone:derived from pSB1K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_102714 Copy
Species:
Genetic Insert: Linker sequence
Vector Backbone Description: Backbone Marker:Self-made; Backbone Size:2267; Vector Backbone:derived from pSB1K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_102710 Copy
Species: Synthetic
Genetic Insert: SpyTag002-MBP
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_102831 Copy
Species:
Genetic Insert: gRNAScaffold_hU6promoter
Vector Backbone Description: Backbone Size:3250; Vector Backbone:Human gRNA Expression Vector / PCR Template for STAgR; Vector Types:Other, as PCR template; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_102843 Copy
Species:
Genetic Insert: STAgR Insert gRNAScaffold_mU6
Vector Backbone Description: Backbone Marker:Stricker Lab; Vector Backbone:PCR Template for STAgR Reactions; Vector Types:Other, PCR Template for STAgR Inserts; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_102844 Copy
Species:
Genetic Insert: STAgR Insert SAM_mU6
Vector Backbone Description: Backbone Marker:Stricker Lab; Vector Backbone:PCR Template for STAgR Reactions; Vector Types:Other, PCR Template for STAgR Inserts; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_102845 Copy
Species:
Genetic Insert: gRNAScaffold_h7SKpromoter
Vector Backbone Description: Vector Backbone:pcDNA1; Vector Types:Other, as PCR template; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_102842 Copy
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