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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pQlox66R Resource Report Resource Website |
RRID:Addgene_135657 | Intron containing lox site | Synthetic | Erythromycin | PMID:31826971 | Use in combination with pQlox71F (addgene 135655) or pQlox71R (addgene 135656), then pQcre1 (addgene 135659). Transcription of LtrB-derived group II intron is driven by the pFer promoter. Redesign intron to target gene using TargeTron or Clostron algorithms (links below), then clone by SOE-PCR or gene synthesis ligation between NdeI and BsrGI sites. Lox66 and Lox71 must be in same orientation to delete the intervening sequence, but the directions of introns relative to one another can generate inverted repeats in the chromosome after deletion has taken place, leading to futher recombinations events. See publication for details. Intron redesign links http://www.clostron.com/clostron1.php http://www.targetrons.com/targetron_pLtrB.php Reference for intron redesign Heap JT, Pennington OJ, Cartman ST, Carter GP, Minton NP. The ClosTron: A universal gene knock-out system for the genus Clostridium. J Microbiol Methods. 2007;70(3):452-464. Perutka J, Wang W, Goerlitz D, Lambowitz AM. Use of Computer-designed Group II Introns to Disrupt Escherichia coli DExH / D-box Protein and DNA Helicase Genes. 2004:421-439. | Backbone Size:6500; Vector Backbone:pAT19; Vector Types:Bacterial Expression, Cre/Lox; Bacterial Resistance:Erythromycin | 2023-04-01 01:02:24 | 0 | |
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pQlox66F Resource Report Resource Website |
RRID:Addgene_135658 | Intron containing lox site | Synthetic | Erythromycin | PMID:31826971 | Use in combination with pQlox71F (addgene 135655) or pQlox71R (addgene 135656), then pQcre1 (addgene 135659). Transcription of LtrB-derived group II intron is driven by the pFer promoter. Redesign intron to target gene using TargeTron or Clostron algorithms (links below), then clone by SOE-PCR or gene synthesis ligation between NdeI and BsrGI sites. Lox66 and Lox71 must be in same orientation to delete the intervening sequence, but the directions of introns relative to one another can generate inverted repeats in the chromosome after deletion has taken place, leading to futher recombinations events. See publication for details. Intron redesign links http://www.clostron.com/clostron1.php http://www.targetrons.com/targetron_pLtrB.php Reference for intron redesign Heap JT, Pennington OJ, Cartman ST, Carter GP, Minton NP. The ClosTron: A universal gene knock-out system for the genus Clostridium. J Microbiol Methods. 2007;70(3):452-464. Perutka J, Wang W, Goerlitz D, Lambowitz AM. Use of Computer-designed Group II Introns to Disrupt Escherichia coli DExH / D-box Protein and DNA Helicase Genes. 2004:421-439. | Backbone Size:6500; Vector Backbone:pAT19; Vector Types:Bacterial Expression, Cre/Lox; Bacterial Resistance:Erythromycin | 2023-04-01 01:02:24 | 0 | |
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pQadd1F Resource Report Resource Website |
RRID:Addgene_135660 | Intron containing lox site | Synthetic | Erythromycin | PMID:31826971 | Transcription of LtrB-derived group II intron is driven by the pFer promoter. Redesign intron to target gene using TargeTron or Clostron algorithms (links below), then clone by SOE-PCR or gene synthesis ligation between NdeI and BsrGI sites. Intron redesign links http://www.clostron.com/clostron1.php http://www.targetrons.com/targetron_pLtrB.php Reference for intron redesign Heap JT, Pennington OJ, Cartman ST, Carter GP, Minton NP. The ClosTron: A universal gene knock-out system for the genus Clostridium. J Microbiol Methods. 2007;70(3):452-464. Perutka J, Wang W, Goerlitz D, Lambowitz AM. Use of Computer-designed Group II Introns to Disrupt Escherichia coli DExH / D-box Protein and DNA Helicase Genes. 2004:421-439. | Backbone Size:6500; Vector Backbone:pAT19; Vector Types:Bacterial Expression, Cre/Lox; Bacterial Resistance:Erythromycin | 2023-04-01 01:02:24 | 0 | |
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pQadd1R Resource Report Resource Website |
RRID:Addgene_135661 | Intron containing lox site | Synthetic | Erythromycin | PMID:31826971 | Transcription of LtrB-derived group II intron is driven by the pFer promoter. Redesign intron to target gene using TargeTron or Clostron algorithms (links below), then clone by SOE-PCR or gene synthesis ligation between NdeI and BsrGI sites. Intron redesign links http://www.clostron.com/clostron1.php http://www.targetrons.com/targetron_pLtrB.php Reference for intron redesign Heap JT, Pennington OJ, Cartman ST, Carter GP, Minton NP. The ClosTron: A universal gene knock-out system for the genus Clostridium. J Microbiol Methods. 2007;70(3):452-464. Perutka J, Wang W, Goerlitz D, Lambowitz AM. Use of Computer-designed Group II Introns to Disrupt Escherichia coli DExH / D-box Protein and DNA Helicase Genes. 2004:421-439. | Backbone Size:6500; Vector Backbone:pAT19; Vector Types:Bacterial Expression, Cre/Lox; Bacterial Resistance:Erythromycin | 2023-04-01 01:02:24 | 0 | |
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pMSP3545 Resource Report Resource Website |
RRID:Addgene_46888 | Erythromycin | PMID:10964628 | This plasmid utilizes the NIsin Controlled Expression (NICE) system. NICE® is a registered trademark of NIZO food research BV, P.O.Box 20, 6710 BA Ede, The Netherlands. pMSP3545 contains the Nisin-inducible PnisA promoter, and the pAMB1 replicon for expression in gram-positive bacteria. It also contains a unique NcoI cleavage site immediately downstream of the nisA ribosomal binding sequence, which may be used for translational fusions, and a rho-independent terminator between the XbaI and XhoI sites of the polylinker. For protein expression in E. faecalis, induce with 10-25 ng/mL nisin (Sigma, N-5764). See associated article for instructions on how to make nisin solution. Addgene's quality control sequencing finds discrepancies with the depositor's provided sequence, but they are not thought to affect plasmid function. | Backbone Size:8539; Vector Backbone:pMSP3545; Vector Types:Bacterial Expression, Other, Nisin-inducible expression; Gram-positive Bacterial Shuttle Vector; Bacterial Resistance:Erythromycin | 2023-04-05 07:54:36 | 0 | |||
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pJB07 Resource Report Resource Website |
RRID:Addgene_190481 | Upstream & Downstream pyrE deletion region | Other | Erythromycin | PMID:36342279 | Vector Backbone:pJB11; Vector Types:Other, E. coli / B. subtilis - C. difficile shuttle vector; Bacterial Resistance:Erythromycin | 2023-07-26 01:04:10 | 0 | ||
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PJEBAN6 Resource Report Resource Website |
RRID:Addgene_203684 | DsRed-Express | Erythromycin | PMID:17014739 | Vector Backbone:pNF8; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | 2023-09-13 01:04:51 | 0 | |||
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pTRKH3-slpGFP Resource Report Resource Website |
RRID:Addgene_27168 | surface (S)-layer protein promoter region fused with modified green fluorescent protein 5 | Lactococcus lactis and synthetic construct | Erythromycin | PMID:20455948 | Backbone Size:7493; Vector Backbone:pTRKH3; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | 2023-09-15 01:10:09 | 0 | ||
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pTRKH3-ermGFP Resource Report Resource Website |
RRID:Addgene_27169 | erythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5 | Enterococcus faecalis and synthetic construct | Erythromycin | PMID:20455948 | Backbone Size:7493; Vector Backbone:pTRKH3; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | presence of an additional 183-bp region at N terminal on insert | 2023-09-15 01:10:09 | 0 | |
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pMRMTK-clo10 Resource Report Resource Website |
RRID:Addgene_203961 | Erythromycin Resistance Gene | Erythromycin | PMID:37712562 | Please visit https://www.biorxiv.org/content/10.1101/2023.06.05.543168v1 for bioRxiv preprint. | Backbone Size:863; Vector Backbone:R6Kγ origin of replication and oriT; Vector Types:Plant Expression; Bacterial Resistance:Erythromycin | 2023-12-07 12:04:56 | 0 | ||
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PJEBAN4 Resource Report Resource Website |
RRID:Addgene_203683 | HcRed | Erythromycin | PMID:17014739 | Vector Backbone:pNF8; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | 2024-02-07 12:04:54 | 0 | |||
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pQmod2E-GG Resource Report Resource Website |
RRID:Addgene_191345 | Plac-RFP (IPTG-inducible red fluorescent protein) | Other | Erythromycin | PMID:36427328 | Backbone Marker:Chain Biotech; Backbone Size:4601; Vector Backbone:pMTL82241; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | 2024-03-19 01:04:49 | 0 | ||
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pQnl_Pcphy23 Resource Report Resource Website |
RRID:Addgene_191354 | Pcphy23-NanoLuc | Synthetic | Erythromycin | PMID:36427328 | Backbone Marker:PMID 19775243; Backbone Size:6972; Vector Backbone:pQexp; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | 2024-03-19 01:04:49 | 0 | ||
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pQmod4E-GG Resource Report Resource Website |
RRID:Addgene_191351 | Plac-RFP (IPTG-inducible red fluorescent protein) | Other | Erythromycin | PMID:36427328 | Backbone Marker:Chain Biotech; Backbone Size:5644; Vector Backbone:pMTL84241; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | 2024-03-19 01:04:49 | 0 | ||
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pEC2935 (p7INT-P23_mNeongreen~ssrA (LDD variant)) Resource Report Resource Website |
RRID:Addgene_218508 | mNeongreen | Synthetic | Erythromycin | PMID:38911550 | This integrative plasmid enables expression of the fluorescence reporter mNeongreen in S. pyogenes. Due to a mutation in the SsrA degradation tag sequence (LAA to LDD), that is translationally fused to the mNeongreen protein, the reporter is destabilised and shows a reduced half-life compare to the untagged mNeongreen protein. The destabilised reporter is advantageous when gene expression is monitored over time as dynamic changes in gene expression (e.g. downregulation) can be observed. See the associated publication for more details. Resource Information: The mNeongreen gene was codon-optimised for expression in S. pyogenes and obtained by gene synthesis. The lactococcal P23 promoter was taken from pLZ12Km2-P23R:TA:ffluc (Plasmid #88900). p7INT was a gift from Prof. Michael Federle (University of Illinois Chicago, USA). The p7INT plasmid integrates into the 3' end of the tmRNA locus in several S. pyogenes strains. It was originally described in: McShan, W.M., McLaughlin, R.E., Nordstrand, A. et al. Vectors containing streptococcal bacteriophage integrases for site-specific gene insertion. Methods Cell Sci 20, 51–57 (1998). https://doi.org/10.1023/A:1009773309163 Please download the detailed plasmid map using the file linked below. | Vector Backbone:p7INT; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | deletion of MCS containing Plac_lacZa, introduction of a mutation to the last three amino acids of the ssrA degradation tag | 2024-08-01 01:07:00 | 0 |
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p3015b Resource Report Resource Website |
RRID:Addgene_209317 | Group B Streptococcus-compatible single guide RNA | Other | Erythromycin | PMID:37296208 | Vector Backbone:vpL3004; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | 2024-08-01 01:06:22 | 0 | ||
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pBsVchDonor Resource Report Resource Website |
RRID:Addgene_203814 | Mini-Tn, aprE cassette | Other | Erythromycin | PMID:37348112 | Plasmid can also be transformed in Bacillus subtilis, grown at 37 C | Vector Backbone:pKS1; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Erythromycin | 2024-11-27 04:06:22 | 0 | |
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pGBScomp Low Resource Report Resource Website |
RRID:Addgene_223203 | N/A | Erythromycin | PMID: | Vector Backbone:pJC005.em; Vector Types:Other, Complementation Vector; Bacterial Resistance:Erythromycin | 2024-12-20 04:08:28 | 0 | |||
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pGBScomp High Resource Report Resource Website |
RRID:Addgene_223202 | N/A | Erythromycin | PMID: | Vector Backbone:pJC005.em; Vector Types:Other, Complementation Vector; Bacterial Resistance:Erythromycin | 2024-12-20 04:08:28 | 0 | |||
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pTlpA_mCherry Resource Report Resource Website |
RRID:Addgene_229146 | mCherry | Other | Erythromycin | PMID:36722614 | Backbone Marker:Addgene ; Backbone Size:2800; Vector Backbone:pLp_3050sNuc; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin | No | 2025-01-30 04:08:24 | 0 |
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