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Species: Caenorhabditis elegans
Genetic Insert: mes-2 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_19397 Copy
Species: Caenorhabditis elegans
Genetic Insert: fbf-1 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_19396 Copy
Species: Caenorhabditis elegans
Genetic Insert: pgl-1 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_19399 Copy
Species: Homo sapiens
Genetic Insert: A1-LCD+7R+12D
Vector Backbone Description: Vector Backbone:pDEST17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_178678 Copy
Species: Homo sapiens
Genetic Insert: NEK3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other, Gateway Donor vector; Bacterial Resistance:Spectinomycin
References:
Comments: The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections.
A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
Proper citation: RRID:Addgene_23821 Copy
Species: Caenorhabditis elegans
Genetic Insert: fbf-1 ORF+3' in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_19602 Copy
Species: Chlamydomonas reinhardtii
Genetic Insert: Channelrhodopsin 2-mCherry
Vector Backbone Description: Backbone Size:5340; Vector Backbone:AAV2; Vector Types:AAV, Cre/Lox, Other, Adeno-Associated Virus; Bacterial Resistance:Ampicillin
References:
Comments: Note: We now recommend the AAV-FLEX-ChR2tdtomato-rev construct. It shows less clumping relative to ChR2 labeled with mCherry.
These vectors are prone to recombination. This is a well known issue with these AAV vectors and is due to the inverted terminal repeats (ITRs) required for AAV production. To minimize recombination, we propagate these plasmids in Stbl2 cells from Invitrogen. Also, to minimize recombination, cells should be cultured at 30 C.
Note that these cultures will grow slowly (20 h for minipreps). Better yields and culture times are obtained with 2xYT as the media. This is strongly recommended.
Because recombination may still happen occasionally, we do a panel of restriction digestions to assess whether the ITRs are in tact. Separate digestions with PvuII, Sma1, and SnaB1 should be performed. The expected patterns can be calculated from the attached sequence.
Proper citation: RRID:Addgene_18916 Copy
Species: Caenorhabditis elegans
Genetic Insert: rme-2 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_19382 Copy
Species: Homo sapiens
Genetic Insert: BRG1
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: Vector: pBabe linearized with SalI and CIP'd. Insert: pBS-CeBRG1 digested with SalI to liberate entire flag tagged BRG1 seq. (Kingston #1359)
Proper citation: RRID:Addgene_1959 Copy
Species: Danio rerio
Genetic Insert: Histone deacetylase 6
Vector Backbone Description: Vector Backbone:pAC8; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_180226 Copy
Species: Caenorhabditis elegans
Genetic Insert: msp-142 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_19379 Copy
Species: Caenorhabditis elegans
Genetic Insert: spe-11 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_19378 Copy
Species: Homo sapiens
Genetic Insert: JMJD3
Vector Backbone Description: Backbone Size:6300; Vector Backbone:pMSCVpuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21214 Copy
Species: Homo sapiens
Genetic Insert: Brg
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pBJ5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid is an expression vector for the full length BRG/BAF190 protein which is similar to Drosphila Brm and contains the conserved ATPase domain in the yeast SWI2/SNF2 protein.
Addgene QC NGS analysis identifies an R1608Q mutation relative to NP_001122316.1.
Proper citation: RRID:Addgene_17873 Copy
Species: Homo sapiens
Genetic Insert: JMJD3
Vector Backbone Description: Backbone Size:6300; Vector Backbone:pMSCVpuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_21212 Copy
Species: Homo sapiens
Genetic Insert: Septin 5
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3791; Vector Backbone:pCMV-Myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_27274 Copy
Species: Homo sapiens
Genetic Insert: A1-LCD-14N-4Q+18G
Vector Backbone Description: Vector Backbone:pDEST17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_178694 Copy
Species: Homo sapiens
Genetic Insert: Septin 5
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3791; Vector Backbone:pCMV-Myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_27275 Copy
Species: Caenorhabditis elegans
Genetic Insert: daz-1 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_19391 Copy
Species: Caenorhabditis elegans
Genetic Insert: fog-2 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_19390 Copy
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