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Species: synthetic gene
Genetic Insert: MSP1E2
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5300; Vector Backbone:pET 28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_20063 Copy
Species: synthetic gene
Genetic Insert: MSP1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5300; Vector Backbone:pET 28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: First described in the following paper: Bayburt, T. H., Grinkova, Y. V., & Sligar, S. G., 2002, Self-assembly of discoidal phospholipid bilayer nanoparticles with membrane scaffold proteins. Nanoletters, 2: 853-856.
Proper citation: RRID:Addgene_20060 Copy
Species: Caenorhabditis elegans
Genetic Insert: mes-3 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_19371 Copy
Species: Caenorhabditis elegans
Genetic Insert: puf-5 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_19372 Copy
Species: Caenorhabditis elegans
Genetic Insert: daz-1 ORF+3' in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_19368 Copy
Species: Caenorhabditis elegans
Genetic Insert: tbb-2 ORF+3' in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_19401 Copy
Species: Caenorhabditis elegans
Genetic Insert: msp-142 ORF+3' in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_19367 Copy
Species: Homo sapiens
Genetic Insert: Septin 5
Vector Backbone Description: Backbone Marker:GE Healthcare Life Sciences; Backbone Size:4985; Vector Backbone:pGEX6P-2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_27269 Copy
Species: Caenorhabditis elegans
Genetic Insert: spn-4 ORF+3' in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_19369 Copy
Species: Caenorhabditis elegans
Genetic Insert: pie-1 ORF+3' in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_19405 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-5 ORF+3' in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_19404 Copy
Species: Caenorhabditis elegans
Genetic Insert: fbf-2 ORF+3' in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_19406 Copy
Species: Homo sapiens
Genetic Insert: PIK4CB
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other, Gateway Donor vector; Bacterial Resistance:Spectinomycin
References:
Comments: The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections.
A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
Addgene QC NGS analysis identifies a G799D mutation relative to NP_001185702.1. As the Kinase ORFeome collection has been generated by high throughput methods using RNA from different tissues and sources, it is possible that this is a tissue specific isoform.
Proper citation: RRID:Addgene_23839 Copy
Species: Homo sapiens
Genetic Insert: NEK5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other, Gateway Donor vector; Bacterial Resistance:Spectinomycin
References:
Comments: The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections.
A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
Proper citation: RRID:Addgene_23434 Copy
Species: Homo sapiens
Genetic Insert: NEK10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other, Gateway Donor vector; Bacterial Resistance:Spectinomycin
References:
Comments: The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections.
A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
Proper citation: RRID:Addgene_23437 Copy
Species: Homo sapiens
Genetic Insert: HK1
Vector Backbone Description: Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags.
This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021
Proper citation: RRID:Addgene_20612 Copy
Species: Homo sapiens
Genetic Insert: COL4A3BP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other, Gateway Donor vector; Bacterial Resistance:Spectinomycin
References:
Comments: The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections.
A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
Proper citation: RRID:Addgene_23569 Copy
Species: Homo sapiens
Genetic Insert: Mutant huamn HKI (with a mutation in the catalytic site in the C-terminal half of the enzyme) in pGFP-N3
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_21919 Copy
Species: Homo sapiens
Genetic Insert: Full length human HKI in pGFP-N3
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_21917 Copy
Species: Homo sapiens
Genetic Insert: NEK3
Vector Backbone Description: Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags.
This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021
Proper citation: RRID:Addgene_20547 Copy
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