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Species: Other
Genetic Insert: LacZ
Vector Backbone Description: Vector Backbone:AV0.1-CS; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124968 Copy
Species: Other
Genetic Insert: gpASNase1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6813; Vector Backbone:pLHCX; Vector Types:Mammalian Expression, Bacterial Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_121526 Copy
Species: Other
Genetic Insert: osTir1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2664; Vector Backbone:pDONR221 P4r-P3r; Vector Types:Synthetic Biology, Other, pMVP Gateway Entry Plasmid; Bacterial Resistance:Kanamycin
References:
Comments: osTir1/9xmyc sequence PCR'd from Addgene #47328
Proper citation: RRID:Addgene_121742 Copy
Species: Other
Genetic Insert: Dam human codon optimized
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:5400; Vector Backbone:p3Flag-EYFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_121909 Copy
Species: Other
Genetic Insert: CI-NpuDnaBC39 intein
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4798; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_121912 Copy
Species: Other
Genetic Insert: Oth-NpuDnaBΔ290 intein
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3981; Vector Backbone:pRSFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_121914 Copy
Species: Other
Genetic Insert: GGPP synthase
Vector Backbone Description: Backbone Size:6200; Vector Backbone:psc101; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
References:
Comments: Based on pAC-LYCipi (Addgene plasmid # 53279 ; http://n2t.net/addgene:53279 ; RRID:Addgene_53279), with backbone changed to pSC101, and crtE swapped for ggpps from Taxus canadensis (truncated & codon optimized).
Please note: The plasmid contains a 387 nucleotide deletion from Trp249 of Eh-idi to His30 of IDI-2 (when compared to the depositor's sequence) combining the two ORFs into a single ORF. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_122017 Copy
Species: Other
Genetic Insert: none
Vector Backbone Description: Backbone Size:6049; Vector Backbone:pUC57 and pWH1266; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_122000 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:de Lorenzo´s lab; Vector Backbone:pSEVA448; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_122096 Copy
Species: Other
Genetic Insert: mNeon/mScarletP2A Rac1-V12
Vector Backbone Description: Backbone Size:6734; Vector Backbone:pCax; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Intent is to introduce low doses of pCAG Cre to recombine a subset of cells, such that mNeon can act as an internal control to mScarlet for use in in utero electroporation or other related techniques. Rac1-V12 is expressed following a P2A site, so that Cre+ cells will be both mScarlet positive and also contain an untagged Rac1-V12.
Note: Addgene NGS is unable to fully resolve the CAG promoter sequence. Please refer to the depositor's sequence for this section of the plasmid.
Proper citation: RRID:Addgene_125136 Copy
Species: Other
Genetic Insert: mNeon/mScarletP2A Rac1-N17
Vector Backbone Description: Backbone Size:6734; Vector Backbone:pCax; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Intent is to introduce low doses of pCAG Cre to recombine a subset of cells, such that mNeon can act as an internal control to mScarlet for use in in utero electroporation or other related techniques. Rac1-N17 is expressed following a P2A site, so that Cre+ cells will be both mScarlet positive and also contain an untagged Rac1-N17.
Note: Addgene NGS is unable to fully resolve the CAG promoter sequence. Please refer to the depositor's sequence for this section of the plasmid.
Proper citation: RRID:Addgene_125137 Copy
Species: Other
Genetic Insert: iNicSnFR3b-(V7)-PM
Vector Backbone Description: Backbone Marker:Janelia; Backbone Size:5005; Vector Backbone:pMinDis.X513; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please note that a 6bp insertion was identified in the NeoR/KanR cassette during Addgene's quality control process. We recommend testing NeoR selection before performing experiments. Vector was received from Janelia HHMI.
Proper citation: RRID:Addgene_125124 Copy
Species: Other
Genetic Insert: iNicSnFR3a-(CC93)-ER
Vector Backbone Description: Backbone Marker:Janelia; Backbone Size:5005; Vector Backbone:pMinDis.X513; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please note that a 6bp insertion and mutation V183A was identified in the NeoR/KanR cassette during Addgene's quality control process. We recommend testing NeoR selection before performing experiments. Vector was received from Janelia HHMI.
Proper citation: RRID:Addgene_125121 Copy
Species: Other
Genetic Insert: FAR protein
Vector Backbone Description: Backbone Size:6457; Vector Backbone:piggyback; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_125127 Copy
Species: Other
Genetic Insert: FAR protein
Vector Backbone Description: Backbone Size:6457; Vector Backbone:piggyback; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_125128 Copy
Species: Other
Genetic Insert: FAR protein
Vector Backbone Description: Backbone Size:6457; Vector Backbone:piggyback; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_125126 Copy
Species: Other
Genetic Insert: FAR protein
Vector Backbone Description: Backbone Size:6457; Vector Backbone:piggyback; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_125129 Copy
Species: Other
Genetic Insert: gRNA and Cas9
Vector Backbone Description: Vector Backbone:pLdCN; Vector Types:CRISPR, Other, Leishmania; Bacterial Resistance:Ampicillin
References:
Comments: This Leishmania CRISPR vector pLdCN2 was specially designed to facilitate cloning two gRNA guide coding sequences into the vector with a single-step digestion-ligation cloning protocol.
Proper citation: RRID:Addgene_125186 Copy
Species: Other
Genetic Insert: LexA
Vector Backbone Description: Backbone Marker:Koen Venken; Vector Backbone:pBS-KS-attB-1-2-PT-SA-SD-0; Vector Types:Other, Other; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_125220 Copy
Species: Other
Genetic Insert: LexA
Vector Backbone Description: Backbone Marker:Koen Venken; Vector Backbone:pBS-KS-attB-1-2-PT-SA-SD-1; Vector Types:Other, Other; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_125221 Copy
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