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Species: Other
Genetic Insert: gRNA
Vector Backbone Description: Vector Backbone:pX330; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124211 Copy
Species: Other
Genetic Insert: rtTAM2-IRES-Hph
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124167 Copy
Species: Other
Genetic Insert: rtTAM2-IRES-Neo
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124166 Copy
Species: Other
Genetic Insert: CheRiff-tdTomato
Vector Backbone Description: Backbone Marker:from Harold Burgess lab (Addgene Plasmid #62215); Backbone Size:4548; Vector Backbone:pT1UciMP; Vector Types:Other, Zebrafish expression; Bacterial Resistance:Ampicillin
References:
Comments: Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CoChR fused to the red fluorescent protein tdTomato. In addition to the opsin gene, it contains tol1 arms, 14xUAS-E1b promoter, ubiquitin C intron, and ocean pout antifreeze protein terminator. Co-inject with Tol1 (not Tol2) transposase mRNA.
Proper citation: RRID:Addgene_124234 Copy
Species: Other
Genetic Insert: MerCreMer-IRES-Zeo
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124184 Copy
Species: Other
Genetic Insert: self-targeting gRNA
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: Please note this plasmid contains Illumina 2nd step primer capture sites within the insert sequence that can affect next generation sequencing results. We suggest using sanger sequencing when working with this plasmid.
Proper citation: RRID:Addgene_124221 Copy
Species: Other
Genetic Insert: SpCas9-HF1
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_124226 Copy
Species: Other
Genetic Insert: mRFP
Vector Backbone Description: Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124266 Copy
Species: Other
Genetic Insert: gp41-1 intein
Vector Backbone Description: Backbone Marker:Delphi Genetics; Backbone Size:2586; Vector Backbone:pSTC3.1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124354 Copy
Species: Other
Genetic Insert: NpuDnaBΔ283 (C1G) mini-intein
Vector Backbone Description: Backbone Marker:Delphi Genetics; Backbone Size:2586; Vector Backbone:pSTC3.1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124353 Copy
Species: Other
Genetic Insert: gp41-1 C-intein
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4774; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124359 Copy
Species: Other
Genetic Insert: dCBE4-gam
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/574020v1 for BioRxiv preprint
Proper citation: RRID:Addgene_124449 Copy
Species: Other
Genetic Insert: Protein A and hyperactive Tn5 transposase (Tnp) fusion protein
Vector Backbone Description: Backbone Size:8079; Vector Backbone:pTXB1-Tn5; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please note that verifying this plasmid by sequencing or digest is challenging due to the presence of the helper plasmid, as multiple bands or mispriming is likely to occur. See Addgene's sequencing results to compare sequences.
This plasmid has been found to be somewhat unstable and prone to concatenation. Concatenation often does not impact plasmid function, but can reduce transformation or transfection efficiencies. If you have trouble isolating the monomeric version of this plasmid, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_124601 Copy
Species: Other
Genetic Insert: Flavodoxin II
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5403; Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124520 Copy
Species: Other
Genetic Insert: NL-DHFR-SpCas9
Vector Backbone Description: Backbone Marker:Feng Zhang; Backbone Size:4000; Vector Backbone:pX330; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124522 Copy
Species: Other
Genetic Insert: Ptet
Vector Backbone Description: Backbone Size:3729; Vector Backbone:pLZ12; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_124768 Copy
Species: Other
Genetic Insert: Ptet
Vector Backbone Description: Backbone Size:3729; Vector Backbone:pLZ12; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_124769 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pCold I; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124890 Copy
Species: Other
Genetic Insert: Cas12a
Vector Backbone Description: Vector Backbone:pCold I; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124891 Copy
Species: Other
Genetic Insert: iNicSnFR3b-(V7)
Vector Backbone Description: Backbone Marker:Janelia; Backbone Size:2827; Vector Backbone:pHHM.X513; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Vector was received from Janelia HHMI
Proper citation: RRID:Addgene_124881 Copy
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