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Species: Other
Genetic Insert: 7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus
Vector Backbone Description: Vector Backbone:pICH47751 ; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177085 Copy
Species: Other
Genetic Insert: P19, CrDXS2, PaGPPS1, and CrGES
Vector Backbone Description: Vector Backbone:pICSL4723; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: L2 P19 + 4X opattB early iridiod chloroplast v6: P1_nosP-TMV-P19-nosterm + P2_4X opattB-TMV-cTP_CrDXS2-masterm + P3_4X opattB-TMV-cTP_PaGPPS1-ocsterm + P4_4X opattB-TMV-cTP_CrGES-g7term)
Proper citation: RRID:Addgene_177088 Copy
Species: Other
Genetic Insert: secologanin synthase (CrSLS1) from Catharanthus roseus
Vector Backbone Description: Vector Backbone:pICH47742 ; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177087 Copy
Species: Other
Genetic Insert: 7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus
Vector Backbone Description: Vector Backbone:pICH47781 ; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177078 Copy
Species: Other
Genetic Insert: geranyl pyrophosphate synthase (PaGPPS1) from Picea abies
Vector Backbone Description: Vector Backbone:pICH47751 ; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177071 Copy
Species: Other
Genetic Insert: geraniol 8-oxidase (CrG8H) from Catharanthus roseus
Vector Backbone Description: Vector Backbone:pICH47751 ; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_177073 Copy
Species: Other
Genetic Insert: sgRNA
Vector Backbone Description: Vector Backbone:pTRV2 (SPDK3888); Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_185629 Copy
Species: Other
Genetic Insert: lacZ
Vector Backbone Description: Vector Backbone:pTRV2 (SPDK3888); Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_185628 Copy
Species: Other
Genetic Insert: lacZ
Vector Backbone Description: Vector Backbone:pTRV2 (SPDK3888); Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_185627 Copy
Species: Other
Genetic Insert: SpCas9
Vector Backbone Description: Vector Backbone:Unspecified; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_185626 Copy
Species: Other
Genetic Insert: SpCas9
Vector Backbone Description: Vector Backbone:Unspecified; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_185625 Copy
Species: Other
Genetic Insert: NbU6-2
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_185624 Copy
Species: Other
Genetic Insert: NbU6-1
Vector Backbone Description: Vector Backbone:pUAP1; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_185623 Copy
Species: Other
Genetic Insert: sgRNA1 -hAAVS1
Vector Backbone Description: Vector Backbone:MTK234_002; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: gRNA used to create hAAVS1 landing pad Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123995 Copy
Species: Other
Genetic Insert: sgRNA for cROSA Homology arm-3
Vector Backbone Description: Vector Backbone:MTK234_002; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123994 Copy
Species: Other
Genetic Insert: sgRNA2 -hAAVS1
Vector Backbone Description: Vector Backbone:MTK234_002; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: gRNA used to create hAAVS1 landing pad Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123996 Copy
Species: Other
Genetic Insert: sg Safetargeting Chr11
Vector Backbone Description: Vector Backbone:MTK234_002; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123984 Copy
Species: Other
Genetic Insert: hROSA26 sg1
Vector Backbone Description: Vector Backbone:MTK234_002; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_123988 Copy
Species: Other
Genetic Insert: PB_pCMV-C-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
Vector Backbone Description: Vector Backbone:MTK0_043; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Plasmid used to generate Fig .2a Please visit https://www.biorxiv.org/content/10.1101/506188v2 for BioRxiv preprint
Proper citation: RRID:Addgene_124006 Copy
Species: Other
Genetic Insert: Threonyl-tRNA ligase
Vector Backbone Description: Vector Backbone:pQE30; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: It is recommended to screen DNA preps from multiple colonies by Sanger sequencing, as this plasmid is prone to deletions in the promoter region.
Proper citation: RRID:Addgene_124119 Copy
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