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Cancer Research UK Scotland Institute Mass Spectrometry Core Facility (RRID:SCR_027652)Copy Citation Copied
URL: https://www.crukscotlandinstitute.ac.uk/advanced-technologies/mass-spectrometry.html
Proper Citation: Cancer Research UK Scotland Institute Mass Spectrometry Core Facility (RRID:SCR_027652)
Description: Core offers proteomics and metabolomics services. Provides support from experimental design to execution, sample processing, data interpretation, and assistance with presentations, manuscript preparation for publication and deposition of data in public repository and data storage on local server. Provides service analysis of post-translational modifications, such as phosphorylation, ubiquitination, acetylation, and others, with precise site localization on either single protein or for entire proteome.Uses Orbitrap Fusion Lumos coupled to Easy-nLC 1200 HPLC system. Facility instruments are operated with various acquisition methods, including classic Data Dependent Analysis (DDA) or more recent Data Independent Analysis (DIA). Acquired data from different methods are used to accurately identify and measure abundance of proteins and peptides employing different quantitation strategies: Label-free, TMT, pulsed-SILAC and Isotope-labelled Iodoacetamide (for Cysteine oxidation).Provides specialized data analysis software like MaxQuant and Mascot for DDA, Spectronaut for DIA of clinical samples and Perseus and Scaffold for data compilation, analysis, and dissemination.
Synonyms: CRUK Scotland Institute Proteomics Facility, CRUK Scotland Institute Metabolomics Facility, Cancer Research UK Scotland Institute Mass Spectrometry Facility
Resource Type: core facility, access service resource, service resource
Keywords: ABRF, Metabolomics, Proteomics, Lipidomics, MSI
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