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URL: https://github.com/liulab-dfci/TRUST4
Proper Citation: TRUST4 (RRID:SCR_026162)
Description: Software tool to analyze TCR and BCR sequences using unselected RNA sequencing data, profiled from fluid and solid tissues, including tumors. Performs de novo assembly on V, J, C genes including the hypervariable complementarity-determining region 3 and reports consensus contigs of BCR/TCR sequences. TRUST4 then realigns the contigs to IMGT reference gene sequences to identify the corresponding gene and CDR3 details. TRUST4 supports both single-end and paired-end bulk or single-cell sequencing data with any read length.
Resource Type: software application, sequence analysis software, source code, data processing software, software resource, data analysis software
Defining Citation: PMID:33986545
Keywords: analyze TCR and BCR sequences, de novo assembly, unselected RNA sequencing data, fluid and solid tissues, tumors,
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