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URL: http://www.addgene.org/218508
Proper Citation: RRID:Addgene_218508
Insert Name: mNeongreen
Organism: Synthetic
Bacterial Resistance: Erythromycin
Defining Citation: PMID:38911550
Vector Backbone Description: Vector Backbone:p7INT; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin
Comments: This integrative plasmid enables expression of the fluorescence reporter mNeongreen in S. pyogenes. Due to a mutation in the SsrA degradation tag sequence (LAA to LDD), that is translationally fused to the mNeongreen protein, the reporter is destabilised and shows a reduced half-life compare to the untagged mNeongreen protein. The destabilised reporter is advantageous when gene expression is monitored over time as dynamic changes in gene expression (e.g. downregulation) can be observed. See the associated publication for more details. Resource Information: The mNeongreen gene was codon-optimised for expression in S. pyogenes and obtained by gene synthesis. The lactococcal P23 promoter was taken from pLZ12Km2-P23R:TA:ffluc (Plasmid #88900). p7INT was a gift from Prof. Michael Federle (University of Illinois Chicago, USA). The p7INT plasmid integrates into the 3' end of the tmRNA locus in several S. pyogenes strains. It was originally described in: McShan, W.M., McLaughlin, R.E., Nordstrand, A. et al. Vectors containing streptococcal bacteriophage integrases for site-specific gene insertion. Methods Cell Sci 20, 51–57 (1998). https://doi.org/10.1023/A:1009773309163 Please download the detailed plasmid map using the file linked below.
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Source: Addgene
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