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URL: http://www.addgene.org/167593
Proper Citation: RRID:Addgene_167593
Insert Name: tonB fs
Organism: Other
Bacterial Resistance: Chloramphenicol
Defining Citation: PMID:
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: The frame-shift of the TonB amphipathic helix was produced by deletion of bp 607 (G) and insertion of a new bp (T) following position 636 of the subsequent tonB open reading frame. Please note that some Genbank listings and Snapgene's ORF recognition software may add additional amino acids to the TonB ORF. Postle and Skare, 1988, PMID: 2839513 demonstrated that the first two Met residues of the open reading frame are not translated and that TonB starts with the third ATG codon. The mutations listed above are numbered based on the functional starting codon.
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Source: Addgene
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