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URL: http://www.addgene.org/100669
Proper Citation: RRID:Addgene_100669
Insert Name: EMMA toolkit Assembly connector for second step assembly
Organism: Synthetic
Bacterial Resistance: Kanamycin
Defining Citation: PMID:28418644
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: DNA element with no function and internal BsaI site generating a ATCC overhang upon cleavage. It is used in the first step BsmBI-based Golden Gate along with HC_Kan_conn_BsaI-1_p1 to flank the vector with BsaI stes. Vectors built in the first BsmBI-based Golden Gate reaction will be flanked by BsaI sites that are used in a second step assembly using as entry vector HC_RFP-4 (the part-entry vector for position 4). See manuscript for futher information
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Source: Addgene
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