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https://en.mgitech.cn/Uploads/Temp/file/20191231/5e0b086c38d24.pdf
Desktop sequencer developed by BGI. Using DNA nanoball and combinational probe anchor synthesis developed from Complete Genomics sequencing technologies, it generates short reads at large scale. BGI/MGI Sequencer.
Proper citation: BGISEQ-500 (RRID:SCR_017979) Copy
https://en.mgitech.cn/products/instruments_info/5/
Sequencer can generate 1-6Tb of high quality data per day, for wide range of applications including Whole Genome Sequencing, Deep Exome Sequencing, Epigenome Sequencing, Transcriptome Sequencing, and targeted panel projects. Powered by DNBSEQTM Technology, DNBSEQ-T7 makes sequencing more efficient and productive with advances in biochemical, fluidics, and optical systems. Detection and characterization of copy number variants based on whole-genome sequencing by MGI platforms.
Proper citation: DNBSEQ-T7 (RRID:SCR_017981) Copy
https://www.mscience.com.au/upload/pages/pacbio/pacbio_rs_ii_brochure.pdf
Sequencer by Pacific Biosciences. System allows scientists to generate finished genome assemblies, reveal and understand epigenomes, and characterize genomic variation. It achieves longest read lengths and highest consensus accuracy. Finishes microbial genomes and improves assembly of larger organisms with multi-kilobase reads and unbiased coverage regardless of GC content. No amplification is required.
Proper citation: PacBio RS II Sequencing System (RRID:SCR_017988) Copy
https://nanoporetech.com/products/promethion
System offers real-time, long-read, direct DNA and RNA sequencing by Oxford Nanopore Technologies. System suitable for large scale projects such as population-scale sequencing or plant genomics, or for large scale service offerings. PromethION 24 and PromethION 48 systems offer ability to run 24 and 48 flow cells respectively. System allows on demand sequencing, using any flow cell at any time. Users can start and stop running individual experiments as required, or deploy multiple flow cells onto single experiments for greater speed or throughput.
Proper citation: PromethION (RRID:SCR_017987) Copy
https://nanoporetech.com/products/gridion
Benchtop sequencing and analysis device designed to run and analyse up to five individually addressable MinION or Flongle Flow Cells by Oxford Nanopore Technologies. Good for labs with multiple projects that need advantages of nanopore sequencing: simple library preparation, real-time analysis and new biological insights from long reads.
Proper citation: GridION Mk1 (RRID:SCR_017986) Copy
https://en.mgitech.cn/products/instruments_info/2/
Sequencer developed by MGI Tech Co. Ltd. Next generation sequencing platform. Its sequencing principle relies on DNB and cPAS technologies also used in previous version of BGISEQ-500 device, but reagents for MGISEQ-2000 are refined and platform utilizes updated software. Built with new chip system that can flexibly support variety of different sequencing models.
Proper citation: DNBSEQ-G400 (RRID:SCR_017980) Copy
Paraffin embedding station that provides constant, reproducible paraffin flow at ten different flow rate settings. All instrument functions, including automatic starting time, are individually programmable at press of button. Compact dimensions, ease of operation, and high standards of convenience. Separately-heated paraffin dispensing system with integrated filter provides constant, reproducible paraffin flow at ten different flow rate settings.
Proper citation: Leica EG1160 Embedding Station (RRID:SCR_018033) Copy
https://www.labx.com/item/molecular-devices-molecular-dynamics-storm-860-molecular/3896212
Multifunction phosphoimager fluorimager laser scanner that collects fluorescent or storage phosphor images from gels, membranes and phosphor screens. It is equipped with blue (450 nm) and red (635 nm) lasers, can scan at resolutions down to 50 microns. It is able to quantify radioactive gels for commonly used isotopes such as 32P, 33P, 125I, 35S, and 14C. There are three detection modes: phosphor screen mode, red/blue fluorescence, chemifluorescence.
Proper citation: Molecular Dynamics Storm 860 Molecular Imager (RRID:SCR_018032) Copy
https://www.pacb.com/blog/introducing-the-sequel-system-the-scalable-platform-for-smrt-sequencing/
Single Molecule, Real-Time sequencer by Pacific Biosciencies. System provides higher throughput, more scalability, reduced footprint and lower sequencing project costs compared to PacBi RS II System, while maintaining benefits of SMRT technology. Ideal for projects such as generating high quality, whole genome de novo assemblies for larger genomes, such as human, plants, and animals. Provides characterization of wide variety of genomic variation types, including those in complex regions not accessible with short-read or synthetic long-range sequencing technologies, while simultaneously revealing epigenetic information.
Proper citation: PacBio Sequel System (RRID:SCR_017989) Copy
https://www.thermofisher.com/order/catalog/product/4315931#/4315931
Genetic analyzer that performs fragment analysis and rapid sequencing applications. Usage per array is 100 runs with use of Applied Biosystems reagents.
Proper citation: Applied Biosystems 3130xl Capillary Array (RRID:SCR_018048) Copy
https://bionano.com/saphyr-systems/
Whole genome imaging instrument by Bionano Genomics for high speed, high throughput structural variant detection and analysis with exceptional sensitivity and specificity. Detects structural variations ranging from 500 bp to megabase pairs in length. For mosaic samples or heterogeneous cancer samples, detects all types of structural variants down to 5% Variant Allele Fraction. Calls repeats and complex rearrangements.
Proper citation: Saphyr (RRID:SCR_017992) Copy
https://www.thelabworldgroup.com/ge-typhoon-9410-microarray-imager
Variable mode imager that operates in three different modes, including phosphorescence, fluorescence, and chemiluminescence. Can scan in visible range using reflected light. Scanning resolution ranges from 1000um down to 10um. Scanning software is linked to ImageQuant TL 7.0 analysis software. ImageQuant is capable of quantifying Typhoon images such as those from Northern or Western blots. Images can be saved in various formats and imported into Powerpoint slides. Instrument has three excitation lasers (532nm, 633nm, 488nm). Instrument utilizes 536SP, 670BP, 520BP emission filters for fluorescence. For DNA, RNA and protein samples you may choose from: storage phosphor auto-radiography, direct blue-excited fluorescence (457-488 nm), direct green-excited fluorescence (532 nm), direct red-excited fluorescence (633 nm), and chemiluminescence.
Proper citation: GE Healthcare 9410 Variable Mode Imager (RRID:SCR_018047) Copy
https://www.leicabiosystems.com/histology-equipment/microtomes/products/leica-rm2265/
Automated rotary microtome that sections tissue samples. Specimen feed system with zero-backlash and maintenance-free cross roller guides and stepper motor operated coarse feed system are located in dust-proof plastic housing. Equipped with safety handwheel with handle that can be centered, as well as mechanical handwheel lock. In manual operation retraction can be adjusted. In motorized operation retraction value varies with selected sectioning speed.
Proper citation: Leica Biosystems RM2265 Fully Automated Rotary Microtome (RRID:SCR_018041) Copy
https://www.leicabiosystems.com/histology-equipment/microtomes/leica-rm2125-rts/
Manual rotary microtome that sections tissue samples. Features include precise feed mechanism, powerBase that ensures stable operation for vibration-free sectioning, advanced knife holder and specimen clamp securely align cutting edge to block, user-selectable coarse feed wheel turn direction and retraction functions, hand wheel, in-built safety guards and rounded shape with arm rests for fatigue-free sectioning,quick-exchange specimen clamp and orientation system for object head allow rapid set up for any block, and two trim steps.
Proper citation: Leica RM2125 RTS Manual Microtome (RRID:SCR_018040) Copy
https://www.thermofisher.com/order/catalog/product/313001R#/313001R
Genetic analyzer can run sequencing and fragment analysis applications including microsatellite analysis, AFLP, LOH, SNP validation, and SNP screening. Maintenance time is reduced by eliminating manual syringe washing and filling with automated polymer delivery. Can increase data quality for sequencing and fragment analysis applications by longer read length, and higher resolution with shorter run times. Uses 96- or 384-well plates with four capillary array.
Proper citation: Applied Biosystems 3130 Genetic Analyzer (RRID:SCR_018046) Copy
https://timothyspringer.org/files/tas/files/biacore3000-instrument.pdf
Biacore 3000 processing unit is an established, label-free system for detailed studies of biomolecular interactions. The system delivers comprehensive characterization of the interaction, answers questions about the rate constants, affinity, specificity, and determines the active concentration of components. The ability to recover and transfer interaction partners directly to MALDI targets for identification and further characterization makes the system highly applicable to fast identification of unknown interactants.
Proper citation: Biacore 3000 Real Time Biomolecular Interaction Analyzer (RRID:SCR_018044) Copy
Bioanalyzer system is automated electrophoresis tool that provides an analytical evaluation of various samples types in many workflows, including next generation sequencing NGS, gene expression, biopharmaceutical, and gene editing research. Digital data is provided in timely manner and delivers assessment of sizing, quantitation, integrity and purity from DNA, RNA, and proteins. Minimal sample volumes are required for accurate result, and data may be exported in many different formats.
Proper citation: Agilent 2100 Bioanalyzer Instrument (RRID:SCR_018043) Copy
http://neuronexus.com/wp-content/uploads/2018/09/nDrive_UserManual.pdf
Microdrive for chronic implantation of neural probe by NeuroNexus.
Proper citation: nDrive (RRID:SCR_018019) Copy
http://www.thunderbiosci.co.kr/wp-content/uploads/2017/07/Apollo-324_brochure.pdf
Compact benchtop platform that enables rapid and full walk-away automation of next generation sequencing applications. Paired with PrepX reagents, Apollo 324 line offers complete sample-toanswer solution for NGS library prep. Apollo 324 automated protocols and PrepX reagent kits combine liquid handling and proven chemistries to produce high quality libraries for all major NGS platforms. System provides complete, walkaway solution using validated protocols and chemistries that produce consistent libraries for DNA-seq, RNA-seq, and ChIP-seq applications.
Proper citation: Wafergen Apollo 324 NGS Library Prep (RRID:SCR_018027) Copy
https://www.selectscience.net/products/gel-logic-212-pro/?prodID=83511#tab-2
Automated gel imaging system for imaging fluorescence or colorimetric data in sample formats such as gels (DNA/Coomassie/Silver stain) membranes, and 96 well plates. Features include autofocusing and directed autoexposure.
Proper citation: Carestream Gel Logic 212 PRO Imaging System (RRID:SCR_018029) Copy
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