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pGAL-flp(H305L)-NAT
 
Resource Report
Resource Website 		RRID:Addgene_160380 FLP1 Saccharomyces cerevisiae Ampicillin PMID: NAT cassette amplified from pLND46 using primers GGT GAT GAC GGT GAA AAC CTC and CTG ATG CGG TAT TTT CTC CTT ACG . Vector flp(H305L)-URA3 (Yeast, Vol. 14: 847–852 (1998))amplified with oligos TGC GTA AGG AGA AAA TAC CGC & GTC AGA GGT TTT CAC CGT CAT outwards from the URA3 region to generate linear fragment with ends complementary to the NAT cassette. Both PCRs carried out using the Primestar MAX polymerase mix (Takara).The two products were then annealed using Gibson assembly, using 3X the amount of NAT PCR product compared to the flp PCR product. 2uL of the mix transformed into NEB 5-alpha competent cells, and transformants were miniprepped and checked for the correct marker replacement with BamH1-Nco1 restriction digest. New insert gives a smaller (1.2 kb) fragment than original vector (1.5kb fragment). Overexpression of flp(H305L) cures 2-micron plasmids. Backbone Marker:Yeast, Vol 14: 847–852 (1998); Backbone Size:6861; Vector Backbone:pBIS-GALkFLP(URA3); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin H305L 2022-12-10 12:02:51 0
pGAL-SKI1-NAT
 
Resource Report
Resource Website 		RRID:Addgene_160382 SKI1 Saccharomyces cerevisiae Ampicillin PMID: Backbone Marker:Genetics. 2009 Sep; 183(1): 365–383.; Backbone Size:5643; Vector Backbone:pLND46; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2022-12-10 12:02:51 0
p11d-sctRPA
 
Resource Report
Resource Website 		RRID:Addgene_102614 RPA1 Saccharomyces cerevisiae Ampicillin PMID:9730822 RPA1 5317 RPA2 7292 RPA3 8284 (start of coding region listed) Vector Backbone:pET11d; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:15:17 0
pG23A
 
Resource Report
Resource Website 		RRID:Addgene_102884 Cdc14/mCherry-AID(71-114)-NatMX Saccharomyces cerevisiae Ampicillin PMID:27989441 This plasmid can be used to dynamically and conditionally deplete Cdc14 in yeast. A characterization guide can be found here: https://www.nature.com/articles/s41598-017-04791-6 Detailed description of plasmid construction can be found in the Supplemental Experimental Procedures of the original paper: http://www.cell.com/molecular-cell/pdfExtended/S1097-2765(16)30726-2 Backbone Marker:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC55758/; Backbone Size:2531; Vector Backbone:HO-poly-KanMX4-HO; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:15:21 0
pJH124
 
Resource Report
Resource Website 		RRID:Addgene_102951 APEX2-NES Saccharomyces cerevisiae Ampicillin PMID:27274088 Backbone Marker:ATCC; Backbone Size:7500; Vector Backbone:pRS425; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:15:23 0
pHES609 [prNOp7-PBAC]
 
Resource Report
Resource Website 		RRID:Addgene_103017 bPAC Saccharomyces cerevisiae Ampicillin PMID:28035051 Vector Backbone:pNH605; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:15:24 0
p413_Csy4NLS
 
Resource Report
Resource Website 		RRID:Addgene_103139 Csy4 Saccharomyces cerevisiae Ampicillin PMID:29161506 Backbone Marker:ATCC; Backbone Size:5522; Vector Backbone:p413 TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:15:26 0
dCas9v2
 
Resource Report
Resource Website 		RRID:Addgene_103140 dCas9-VPR Saccharomyces cerevisiae Ampicillin PMID:29161506 Vector Backbone:pDTU-113; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Removed scaffold gRNA from original plasmid 2022-04-22 03:15:26 0
pCRA03
 
Resource Report
Resource Website 		RRID:Addgene_103141 gRNAs Saccharomyces cerevisiae Ampicillin PMID:29161506 Vector Backbone:dCas9v2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:15:26 0
Rp51a Hyperstabilized 5'SS
 
Resource Report
Resource Website 		RRID:Addgene_100988 RP51A Saccharomyces cerevisiae Ampicillin PMID:28829039 Vector Backbone:pUC18; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin 5'ss mut ATG/GTATGT -> AAG/GTAAGT 2022-04-22 03:14:59 0
Rp51a_10bp 5'SS_No BP or pBP_pUC18
 
Resource Report
Resource Website 		RRID:Addgene_100989 RP51A Saccharomyces cerevisiae Ampicillin PMID:28829039 Vector Backbone:pUC18; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin tGGTAtGTta->AGGTAAGTAT 5'SS mutant, TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutation 2022-04-22 03:14:59 0
RP51A-NoBS-NoPseudoBS
 
Resource Report
Resource Website 		RRID:Addgene_100986 RP51A Saccharomyces cerevisiae Ampicillin PMID:28829039 Vector Backbone:pUC18; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin BS mutated to GTTAGTG, pseudo BS mutated to GTTTGTG 2022-04-22 03:14:59 0
Rp51a_SUS1 5'ss_No_BP_pBP-pUC18
 
Resource Report
Resource Website 		RRID:Addgene_100990 RP51A Saccharomyces cerevisiae Ampicillin PMID:28829039 Vector Backbone:pUC18; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin GGTAtGT->tGTAtGa 5'SS mutant, TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutation 2022-04-22 03:14:59 0
Rp51a_SUS1 5'SS
 
Resource Report
Resource Website 		RRID:Addgene_100991 RP51A Saccharomyces cerevisiae Ampicillin PMID:28829039 Vector Backbone:pUC18; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin GGTAtGT->tGTAtGa 5'SS mutant 2022-04-22 03:14:59 0
pUDP010
 
Resource Report
Resource Website 		RRID:Addgene_101166 HH-gRNA-HDV targetting SeILV6 in S. pastorianus Saccharomyces cerevisiae Ampicillin PMID:29207996 Backbone Size:5868; Vector Backbone:pUDP003; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2022-04-22 03:15:02 0
pUDP012
 
Resource Report
Resource Website 		RRID:Addgene_101167 HH-gRNA-HDV targetting SeILV6 in S. pastorianus Saccharomyces cerevisiae Ampicillin PMID:29207996 Backbone Marker:JM Daran; Backbone Size:10664; Vector Backbone:pUDP004; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2022-04-22 03:15:02 0
pUDP044
 
Resource Report
Resource Website 		RRID:Addgene_101168 polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus Saccharomyces cerevisiae Ampicillin PMID:29207996 Backbone Marker:JM Daran; Backbone Size:10664; Vector Backbone:pUDP004; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2022-04-22 03:15:02 0
pDONR221-Sgd1
 
Resource Report
Resource Website 		RRID:Addgene_100000 Sgd1 Saccharomyces cerevisiae Kanamycin PMID:29054886 Addgene NGS results found T307A, S332F, and V832F in the Sgd1 translation compared to the best-match NCBI reference sequence (GAA25198.1). Vector Backbone:pDONR221; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin 2022-04-22 03:14:45 0
pACT2-Dhr1
 
Resource Report
Resource Website 		RRID:Addgene_100003 Dhr1 Saccharomyces cerevisiae Ampicillin PMID:29054886 Addgene NGS results found S1073N, and an extra 16 amino acids at the C-terminus of the Dhr1 translation compared to NP_013847.1. Vector Backbone:pACT2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:14:46 0
pAS2-1-Dhr1
 
Resource Report
Resource Website 		RRID:Addgene_100002 Dhr1 Saccharomyces cerevisiae Ampicillin PMID:29054886 Addgene NGS results found S1073N, and an extra 30 amino acids at the C-terminus of the Dhr1 translation compared to NP_013847.1. Vector Backbone:pAS2-1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:14:45 0

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