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Plasmids are provided by Addgene and DGRC.

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  • Organism:gallus gallus (facet)


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611 Results - per page

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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
PresentER-SIINFEKL (mCherry)
 
Resource Report
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RRID:Addgene_102945 SIINFEKL Gallus gallus Ampicillin PMID:30499773 Please visit https://www.biorxiv.org/content/early/2018/09/22/267047 for bioRxiv preprint. Backbone Size:8200; Vector Backbone:PresentER; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin OVA257-264 2022-04-22 03:15:22 0
pCAGGS-FuG-E (P440E)
 
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RRID:Addgene_119978 Fusion Glycoprotein type E (P440E) Gallus gallus Ampicillin PMID:30347222 Addgene NGS was unable to fully resolve the sequence of the CAGGS promoter. Please refer to the depositor's sequence for this section of the plasmid. Backbone Size:4900; Vector Backbone:pCAGGS vector; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2022-04-22 03:20:28 0
pET15b/GgVcl V1-851 (aka Vh)
 
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RRID:Addgene_46172 Vcl 1-851 Gallus gallus Ampicillin PMID:15728584 Using in-frame primers containing 5′-NdeI and 3′-XhoI sites, appropriate regions of the chicken vinculin cDNA sequence were amplified by PCR followed by addition of 3′-adenosine overhangs with Taq polymerase. The PCR products were ligated into the TOPO II plasmid (Invitrogen) and then subcloned into pET15b His tag expression vector (Novagen, Madison, WI) using NdeI and XhoI digestion of the multiple cloning site. Backbone Marker:EMD; Backbone Size:5700; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin aa 1-851 (aka Vh) head domain 2022-09-09 01:03:24 0
pET15b/GgVcl V1-258 (aka VD1)
 
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RRID:Addgene_46173 Vcl 1-258 Gallus gallus Ampicillin PMID:15728584 Using in-frame primers containing 5′-NdeI and 3′-XhoI sites, appropriate regions of the chicken vinculin cDNA sequence were amplified by PCR followed by addition of 3′-adenosine overhangs with Taq polymerase. The PCR products were ligated into the TOPO II plasmid (Invitrogen) and then subcloned into pET15b His tag expression vector (Novagen, Madison, WI) using NdeI and XhoI digestion of the multiple cloning site. Backbone Marker:EMD; Backbone Size:5700; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin aa 1-258 (VD1) 2022-09-09 01:03:24 0
pET15b/GgVcl 1-1066 (full length)
 
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RRID:Addgene_46171 Vcl Gallus gallus Ampicillin PMID:15195105 Backbone Marker:EMD; Backbone Size:5700; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2022-09-09 01:03:24 0
pET15b/GgVcl 884-1066 (aka Vt)
 
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RRID:Addgene_46176 Vcl 884-1066 Gallus gallus Ampicillin PMID:10373448 Using in-frame primers containing 5′-NdeI and 3′-XhoI sites, appropriate regions of the chicken vinculin cDNA sequence were amplified by PCR followed by addition of 3′-adenosine overhangs with Taq polymerase. The PCR products were ligated into the TOPO II plasmid (Invitrogen) and then subcloned into pET15b His tag expression vector (Novagen, Madison, WI) using NdeI and XhoI digestion of the multiple cloning site. Backbone Marker:EMD; Backbone Size:5700; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin aa 884-1066 (tail domain) 2022-09-09 01:03:24 0
pET15b/GgVcl 1-851 A50I mutation
 
Resource Report
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RRID:Addgene_46174 Vcl 1-851 A50I Gallus gallus Ampicillin PMID:16608855 See Addgene plasmid 46172 for wild-type cloning information. The A50I mutation was introduced by QuikChange mutagenesis (Stratagene). Backbone Marker:EMD; Backbone Size:5700; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin aa 1-851 A50I mutation (talin binding domain mutant in vinculin head domain) 2022-09-09 01:03:24 0
pPaxillin-Electra2
 
Resource Report
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RRID:Addgene_184926 paxillin-Electra2 Gallus gallus Kanamycin PMID:35715437 Backbone Size:3933; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2022-09-09 01:02:34 0
pET15b/GgVcl 1-258 A50I mutation
 
Resource Report
Resource Website
RRID:Addgene_46175 Vcl 1-258 A50I Gallus gallus Ampicillin PMID:16608855 See Addgene plasmid 46173 for wild-type cloning information. The A50I mutation was introduced by QuikChange mutagenesis (Stratagene). Backbone Marker:EMD; Backbone Size:5700; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin aa 1-258 (VD1) A50I mutation; talin binding deficient 2022-09-09 01:03:24 0
p401F/GgVcl 1-1066 (N term FLAG tag)
 
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RRID:Addgene_46284 Vcl 1-1066 Gallus gallus Ampicillin PMID: Backbone Marker:described in N. Kioka. 1999. JCB 144:59-69; Vector Backbone:p401F; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-09-09 01:03:25 0
pET15b/GVcl 1-1066 /*TBM
 
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RRID:Addgene_162781 Gallus gallus vinculin Gallus gallus Ampicillin PMID: Vector Backbone:pET15b/GVcl 1-1066 (Addgene #46171); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin changed Gly 454 to Val 2022-09-09 01:01:58 0
mEos3.2-CCPB1-C-18
 
Resource Report
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RRID:Addgene_57448 CCPB1 Gallus gallus Kanamycin PMID: . Excitation = 507 / 572; Emission = 516 / 580 Backbone Size:4750; Vector Backbone:mEos3.2-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2022-09-21 01:03:50 0
pBa.NgCAM-Halo
 
Resource Report
Resource Website
RRID:Addgene_162719 NgCam Gallus gallus Ampicillin PMID:32959500 pBa vector is susceptible to recombination and should not be grown in fast growing bacteria or cloned using recombination. Vector Backbone:pBeta Actin-Halo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin E137K, K138E, and K642E 2022-09-21 01:02:00 0
Paxillin_PSCFP2_ mEOS2
 
Resource Report
Resource Website
RRID:Addgene_114192 Paxillin Gallus gallus Kanamycin PMID: Vector Backbone:pmEOS2-Paxillin; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2022-04-22 03:18:33 0
VinculinTS-A50I/I997A
 
Resource Report
Resource Website
RRID:Addgene_111829 VinculinTS (I997A) Gallus gallus Ampicillin PMID:29642037 Backbone Marker:Invitrogen; Backbone Size:5363; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin changed vinculin alanine 50 to isoleucine and isoleucine 997 to alanine 2022-04-22 03:17:57 0
VinculinTS-I997A
 
Resource Report
Resource Website
RRID:Addgene_111828 VinculinTS (I997A) Gallus gallus Ampicillin PMID:29642037 Backbone Marker:Invitrogen; Backbone Size:5360; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin changed isoleucine 997 to alanine 2022-04-22 03:17:57 0
VinTS-tCRMod-GGSGGS7
 
Resource Report
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RRID:Addgene_111764 Clover-mRuby2-based vinculin tension sensor with (GGSGGS)7 extensible domain Gallus gallus Ampicillin PMID:30024378 Partially based on Addgene Plasmids #26019, #49089, and #21769 Backbone Marker:Invitrogen; Backbone Size:5399; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Residues 1-228 of Clover, residues 1-3 deleted from mRuby2 2022-04-22 03:17:55 0
pCMV-Y-RAN-RFP6
 
Resource Report
Resource Website
RRID:Addgene_106424 Y-RAN-RFP6 Gallus gallus Kanamycin PMID:29440485 Backbone Marker:Takara/Clontech; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2022-04-22 03:16:17 0
pet 3d beta tentacle delete CP
 
Resource Report
Resource Website
RRID:Addgene_13300 capping protein alpha 1 beta 1 (deletion 28 amino acids ) Gallus gallus Ampicillin PMID:12956956 Soeno et al 1998; journal of muscle research and cell motility 19, 639-646 Backbone Marker:novagen; Backbone Size:5000; Vector Backbone:pET- 3d; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin CP alpha subunit is intact with a mutated beta C-termiinus (delete 28 amino acids) 2022-04-22 03:24:43 0
pet 3d alpha tentacle delete CP
 
Resource Report
Resource Website
RRID:Addgene_13299 capping protein alpha 1 (deletion 28 amino acids) beta 1 subunits Gallus gallus Ampicillin PMID:12956956 Soeno et al 1998; journal of muscle research and cell motility 19, 639-646 Backbone Marker:novagen; Backbone Size:5000; Vector Backbone:pET- 3d; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2022-04-22 03:24:43 0

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