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http://www.addgene.org/19679

Species: Caenorhabditis elegans
Genetic Insert: Gateway(R2-R1)-L4440
Vector Backbone Description: Backbone Size:0; Vector Backbone:L4440; Vector Types:RNAi, Other, Gateway destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Sequence mismatches between Addgene sequence and depositor sequence do not affect function.

Proper citation: RRID:Addgene_19679 Copy   

•   Source: Addgene

http://www.addgene.org/19703

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6938; Vector Backbone:pCDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_19703 Copy   

•   Source: Addgene

http://www.addgene.org/22423

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:5808; Vector Backbone:pCS2+; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: pCS2+ was digested with Stu I and ligated with the Reading Frame B Gateway cassette

Proper citation: RRID:Addgene_22423 Copy   

•   Source: Addgene

http://www.addgene.org/22424

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:5800; Vector Backbone:pCS2+; Vector Types:Other, Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: pCSDest was digested with PstI and XhoI to remove the attR2 site and was replaced with an attR3 site from pDESTR4-R3

Proper citation: RRID:Addgene_22424 Copy   

•   Source: Addgene

http://www.addgene.org/20349

Species: cloning vector
Genetic Insert: pWS-TK3
Vector Backbone Description: Backbone Size:6379; Vector Backbone:N/A; Vector Types:Mouse Targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Please note that Addgene's quality control sequencing indicates that the PacI and SgfI sites present in the depositor's map are not present in the plasmid's actual sequence.

Proper citation: RRID:Addgene_20349 Copy   

•   Source: Addgene

http://www.addgene.org/20350

Species: cloning vector
Genetic Insert: pWS-TK6
Vector Backbone Description: Backbone Size:6355; Vector Backbone:N/A; Vector Types:Mouse Targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Please note that Addgene's quality control sequencing indicates that the PacI and SgfI sites present in the depositor's map are not present in the plasmid's actual sequence.

Proper citation: RRID:Addgene_20350 Copy   

•   Source: Addgene

http://www.addgene.org/20870

Species: bacteria
Genetic Insert: aphA::cat
Vector Backbone Description: Backbone Marker:Skorupski, K., Taylor, R.K.; Backbone Size:4400; Vector Backbone:pKAS32; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Contains cat which confers resistance to chloramphenicol.

Proper citation: RRID:Addgene_20870 Copy   

•   Source: Addgene

http://www.addgene.org/20960

Species:
Genetic Insert: Gateway Destination Vector
Vector Backbone Description: Backbone Size:6465; Vector Backbone:PB-CA; Vector Types:Mammalian Expression, piggyBac; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_20960 Copy   

•   Source: Addgene

http://www.addgene.org/21189

Species:
Genetic Insert: GateWay conversion cassette
Vector Backbone Description: Backbone Size:14820; Vector Backbone:pRosa26-PA; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: pRosa26-DEST was made by inserting a GateWay conversion cassette into the XhoI site of pBigT, followed by transfer of PacI / AscI fragment of the resulting vector to the pRosa26-PA vector (see http://www.biomedcentral.com/1471-213X/1/4 for details on these vectors). The resulting vector resembles the construct that was used for the R26R Cre reporter mouse made by Phil Soriano in which the expression of lacZ is coupled to the endogenous Rosa26 locus via a splice acceptor site, but only after removal of the lox-STOP-lox cassette (which is actually the neo-R cassette used during the targeting). The only thing changed from the pBigT vectors and the R26R reporter is the cloning strategy, so the resulting knock-in should be as good as these systems, and as good (or as bad) as the use of the Rosa26 locus in any situation. Stbl3 cells are advisable to use after users have done the LR recombination reaction. See http://www.hgu.mrc.ac.uk/people/n.hastie_rosa.html for more information and protocols for this plasmid. Further advice from the depositing lab: "Grow single colonies in Amp + Cam at 30 C, definitively not 37. Including the Cam should ensure that spontaneous recombinants that loose the Gateway cassette don't survive... - Do minipreps and test with the double digest PacI / AscI. This should give 4.9 kb + 9.8 kb. If this is okay, the vector should be okay" Please note that there are discrepancies between Addgene's quality control sequence and the theoretical sequence supplied by the depositing lab. These discrepancies should not affect the function of the construct.

Proper citation: RRID:Addgene_21189 Copy   

•   Source: Addgene

http://www.addgene.org/25943

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5566; Vector Backbone:pACT; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_25943 Copy   

•   Source: Addgene

http://www.addgene.org/25945

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5566; Vector Backbone:pACT; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_25945 Copy   

•   Source: Addgene

http://www.addgene.org/25941

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5566; Vector Backbone:pACT; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_25941 Copy   

•   Source: Addgene

http://www.addgene.org/26302

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:7401; Vector Backbone:pCG150; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Use for puromycin selection of transgenes in C. elegans. This vector is identical to pBCN21-R4R3, but a 8xC183_minPmyo-2::mCherry::unc-54_3'UTR cassette was inserted in the backbone as an additional visual marker. This is a Gateway 3-fragment compatible destination vector. It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).

Proper citation: RRID:Addgene_26302 Copy   

•   Source: Addgene

http://www.addgene.org/26303

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:8090; Vector Backbone:pCG150; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Use for puromycin selection of transgenes in Caenorhabditis species other than C. elegan (works also in C. elegans). This vector is the same as pBCN22-R4R3 but with a different Pmyo-2::mCherry::unc-54_3'UTR construct that gives good fluoresence expression in the pharynx of C. elegans as well as other species, e.g. C. briggsae, C. remanei and C. brenneri. This is a Gateway 3-fragment compatible destination vector. It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).

Proper citation: RRID:Addgene_26303 Copy   

•   Source: Addgene

http://www.addgene.org/26228

Species: Saccharomyces cerevisiae
Genetic Insert: GAL4::VP16
Vector Backbone Description: Backbone Size:8770; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_26228 Copy   

•   Source: Addgene

http://www.addgene.org/26227

Species: Saccharomyces cerevisiae
Genetic Insert: GAL4
Vector Backbone Description: Backbone Size:9223; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_26227 Copy   

•   Source: Addgene

http://www.addgene.org/26232

Species: Saccharomyces cerevisiae
Genetic Insert: nlsLexA::GADfl
Vector Backbone Description: Backbone Size:8747; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_26232 Copy   

•   Source: Addgene

http://www.addgene.org/26360

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_26360 Copy   

•   Source: Addgene

http://www.addgene.org/26415

Species:
Genetic Insert: attR4-ccdB-attR2
Vector Backbone Description: Backbone Size:8623; Vector Backbone:pDP#MM016b; Vector Types:Bacterial Expression, Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: A KpnI-ApaI fragment containing the GFP encoding sequence and the unc-54 3′ UTR from pPD95.79 was cloned into pDP#MM016b. An EcoRV fragment of the attR4-ccdB-attR2 cassette from pDEST-6 (Invitrogen) was cloned into this new construct digested with Acc65I and filled in with Klenow. The unc-119 rescuing fragment present in these vectors can be used as a marker to select transgenic worms.

Proper citation: RRID:Addgene_26415 Copy   

•   Source: Addgene

http://www.addgene.org/26414

Species:
Genetic Insert: attR4-ccdB-attR2 cassette
Vector Backbone Description: Backbone Size:8623; Vector Backbone:pDP#MM016b; Vector Types:Bacterial Expression, Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: A HindIII-KpnI fragment containing the attR4-ccdB-attR2 cassette from pDEST-6 (Invitrogen) was cloned into the unc-119 rescue vector pDP#MM016b. The unc-119 rescuing fragment present in these vectors can be used as a marker to select transgenic worms.

Proper citation: RRID:Addgene_26414 Copy   

•   Source: Addgene