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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
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|---|---|---|---|---|---|---|---|---|---|---|
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pGC48 Resource Report Resource Website |
RRID:Addgene_19679 | Gateway(R2-R1)-L4440 | Caenorhabditis elegans | Chloramphenicol and Ampicillin | PMID:18723890 | Sequence mismatches between Addgene sequence and depositor sequence do not affect function. | Backbone Size:0; Vector Backbone:L4440; Vector Types:RNAi, Other, Gateway destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin | 2022-04-22 03:35:27 | 0 | |
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pgLAP2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_19703 | Chloramphenicol and Ampicillin | PMID: | Backbone Marker:Invitrogen; Backbone Size:6938; Vector Backbone:pCDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2022-04-22 03:35:28 | 1 | ||||
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pCSDest Resource Report Resource Website |
RRID:Addgene_22423 | Chloramphenicol and Ampicillin | PMID:17948311 | pCS2+ was digested with Stu I and ligated with the Reading Frame B Gateway cassette | Backbone Size:5808; Vector Backbone:pCS2+; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin | 2022-04-22 03:36:02 | 0 | |||
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pCSDest2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_22424 | Chloramphenicol and Ampicillin | PMID:17948311 | pCSDest was digested with PstI and XhoI to remove the attR2 site and was replaced with an attR3 site from pDESTR4-R3 | Backbone Size:5800; Vector Backbone:pCS2+; Vector Types:Other, Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin | 2022-04-22 03:36:02 | 1 | |||
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pWS-TK3 Resource Report Resource Website |
RRID:Addgene_20349 | pWS-TK3 | cloning vector | Chloramphenicol and Ampicillin | PMID:18546598 | Please note that Addgene's quality control sequencing indicates that the PacI and SgfI sites present in the depositor's map are not present in the plasmid's actual sequence. | Backbone Size:6379; Vector Backbone:N/A; Vector Types:Mouse Targeting; Bacterial Resistance:Chloramphenicol and Ampicillin | 2022-04-22 03:35:39 | 0 | |
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pWS-TK6 Resource Report Resource Website |
RRID:Addgene_20350 | pWS-TK6 | cloning vector | Chloramphenicol and Ampicillin | PMID:18546598 | Please note that Addgene's quality control sequencing indicates that the PacI and SgfI sites present in the depositor's map are not present in the plasmid's actual sequence. | Backbone Size:6355; Vector Backbone:N/A; Vector Types:Mouse Targeting; Bacterial Resistance:Chloramphenicol and Ampicillin | 2022-04-22 03:35:39 | 0 | |
|
pIOM17 Resource Report Resource Website |
RRID:Addgene_20870 | aphA::cat | bacteria | Chloramphenicol and Ampicillin | PMID:16750581 | Contains cat which confers resistance to chloramphenicol. | Backbone Marker:Skorupski, K., Taylor, R.K.; Backbone Size:4400; Vector Backbone:pKAS32; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2022-04-22 03:35:43 | 0 | |
|
PB-CA Resource Report Resource Website |
RRID:Addgene_20960 | Gateway Destination Vector | Chloramphenicol and Ampicillin | PMID:19252478 | Backbone Size:6465; Vector Backbone:PB-CA; Vector Types:Mammalian Expression, piggyBac; Bacterial Resistance:Chloramphenicol and Ampicillin | 2022-04-22 03:35:44 | 0 | |||
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pRosa26-DEST Resource Report Resource Website |
RRID:Addgene_21189 | GateWay conversion cassette | Chloramphenicol and Ampicillin | PMID:19014667 | pRosa26-DEST was made by inserting a GateWay conversion cassette into the XhoI site of pBigT, followed by transfer of PacI / AscI fragment of the resulting vector to the pRosa26-PA vector (see http://www.biomedcentral.com/1471-213X/1/4 for details on these vectors). The resulting vector resembles the construct that was used for the R26R Cre reporter mouse made by Phil Soriano in which the expression of lacZ is coupled to the endogenous Rosa26 locus via a splice acceptor site, but only after removal of the lox-STOP-lox cassette (which is actually the neo-R cassette used during the targeting). The only thing changed from the pBigT vectors and the R26R reporter is the cloning strategy, so the resulting knock-in should be as good as these systems, and as good (or as bad) as the use of the Rosa26 locus in any situation. Stbl3 cells are advisable to use after users have done the LR recombination reaction. See http://www.hgu.mrc.ac.uk/people/n.hastie_rosa.html for more information and protocols for this plasmid. Further advice from the depositing lab: "Grow single colonies in Amp + Cam at 30 C, definitively not 37. Including the Cam should ensure that spontaneous recombinants that loose the Gateway cassette don't survive... - Do minipreps and test with the double digest PacI / AscI. This should give 4.9 kb + 9.8 kb. If this is okay, the vector should be okay" Please note that there are discrepancies between Addgene's quality control sequence and the theoretical sequence supplied by the depositing lab. These discrepancies should not affect the function of the construct. | Backbone Size:14820; Vector Backbone:pRosa26-PA; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2022-04-22 03:35:46 | 0 | ||
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pMAD-Gate4 Resource Report Resource Website |
RRID:Addgene_25943 | Chloramphenicol and Ampicillin | PMID:20515414 | Backbone Marker:Promega; Backbone Size:5566; Vector Backbone:pACT; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2022-04-22 03:37:02 | 0 | ||||
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pMAD-Gate6 Resource Report Resource Website |
RRID:Addgene_25945 | Chloramphenicol and Ampicillin | PMID:20515414 | Backbone Marker:Promega; Backbone Size:5566; Vector Backbone:pACT; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2022-04-22 03:37:02 | 0 | ||||
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pMAD-Gate2 Resource Report Resource Website |
RRID:Addgene_25941 | Chloramphenicol and Ampicillin | PMID:20515414 | Backbone Marker:Promega; Backbone Size:5566; Vector Backbone:pACT; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2022-04-22 03:37:02 | 0 | ||||
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pBCN22-R4R3 Resource Report Resource Website |
RRID:Addgene_26302 | Chloramphenicol and Ampicillin | PMID:20729840 | Use for puromycin selection of transgenes in C. elegans. This vector is identical to pBCN21-R4R3, but a 8xC183_minPmyo-2::mCherry::unc-54_3'UTR cassette was inserted in the backbone as an additional visual marker. This is a Gateway 3-fragment compatible destination vector. It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm). | Backbone Size:7401; Vector Backbone:pCG150; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2022-04-22 03:37:06 | 0 | |||
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pBCN28-R4R3 Resource Report Resource Website |
RRID:Addgene_26303 | Chloramphenicol and Ampicillin | PMID:20729840 | Use for puromycin selection of transgenes in Caenorhabditis species other than C. elegan (works also in C. elegans). This vector is the same as pBCN22-R4R3 but with a different Pmyo-2::mCherry::unc-54_3'UTR construct that gives good fluoresence expression in the pharynx of C. elegans as well as other species, e.g. C. briggsae, C. remanei and C. brenneri. This is a Gateway 3-fragment compatible destination vector. It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm). | Backbone Size:8090; Vector Backbone:pCG150; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2022-04-22 03:37:06 | 0 | |||
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pBPGAL4.2::VP16Uw Resource Report Resource Website 1+ mentions |
RRID:Addgene_26228 | GAL4::VP16 | Saccharomyces cerevisiae | Chloramphenicol and Ampicillin | PMID:20697123 | Backbone Size:8770; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2022-04-22 03:37:05 | 1 | ||
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pBPGAL4.2Uw-2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_26227 | GAL4 | Saccharomyces cerevisiae | Chloramphenicol and Ampicillin | PMID:20697123 | Backbone Size:9223; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2022-04-22 03:37:05 | 1 | ||
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pBPnlsLexA::GADflUw Resource Report Resource Website 1+ mentions |
RRID:Addgene_26232 | nlsLexA::GADfl | Saccharomyces cerevisiae | Chloramphenicol and Ampicillin | PMID:20697123 | Backbone Size:8747; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2022-04-22 03:37:05 | 2 | ||
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pFRT_DESTFLAGHA Resource Report Resource Website |
RRID:Addgene_26360 | Chloramphenicol and Ampicillin | PMID:21344536 | Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2022-04-22 03:37:06 | 0 | ||||
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pdestMB14 Resource Report Resource Website |
RRID:Addgene_26415 | attR4-ccdB-attR2 | Chloramphenicol and Ampicillin | PMID:15489340 | A KpnI-ApaI fragment containing the GFP encoding sequence and the unc-54 3′ UTR from pPD95.79 was cloned into pDP#MM016b. An EcoRV fragment of the attR4-ccdB-attR2 cassette from pDEST-6 (Invitrogen) was cloned into this new construct digested with Acc65I and filled in with Klenow. The unc-119 rescuing fragment present in these vectors can be used as a marker to select transgenic worms. | Backbone Size:8623; Vector Backbone:pDP#MM016b; Vector Types:Bacterial Expression, Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2022-04-22 03:37:07 | 0 | ||
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pdestDD03 Resource Report Resource Website |
RRID:Addgene_26414 | attR4-ccdB-attR2 cassette | Chloramphenicol and Ampicillin | PMID:15489340 | A HindIII-KpnI fragment containing the attR4-ccdB-attR2 cassette from pDEST-6 (Invitrogen) was cloned into the unc-119 rescue vector pDP#MM016b. The unc-119 rescuing fragment present in these vectors can be used as a marker to select transgenic worms. | Backbone Size:8623; Vector Backbone:pDP#MM016b; Vector Types:Bacterial Expression, Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2022-04-22 03:37:07 | 0 |
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