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Species:
Genetic Insert: FRT-cat-FRT
Vector Backbone Description: Backbone Marker:M. Koob (University of Wisconsin, Madison); Vector Backbone:pANTSγ; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: This is a template plasmids for frt-flanked cat cassette. The cat cassette originally came from pSC140.
pKD3 and pKD4 are identical except for the region between the FRT sites, so they create an identical scar that has stop codons in all six reading frames. As drawn in the map, this scar has an idealized ribosome binding site and start codon for downstream (rightward) gene expression.
Proper citation: RRID:Addgene_45604 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:0; Vector Backbone:N/A; Vector Types:Other, N/A; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_40802 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Addgene plasmid 31829; Vector Backbone:pminiTol2; Vector Types:Other, Gateway destination; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: The plasmid contains Tol2 transposon.
Proper citation: RRID:Addgene_40970 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:6500; Vector Backbone:MSCV-N-Flag-HA-IRES-PURO; Vector Types:Mammalian Expression, Retroviral, Other, Gateway Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_41033 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:4146; Vector Backbone:pTKIP; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_41065 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:3267; Vector Backbone:pKD4; Vector Types:Other, E.coli genomic integration; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: This plasmid is used for integration of DNA into the E.coli genome via homologous recombination. The DNA of interest is cloned between lacZ homologous arms and integrated at the lacZ locus.
Proper citation: RRID:Addgene_46764 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:3267; Vector Backbone:pKD4; Vector Types:Other, E.coli genomic integration; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: This plasmid is used for integration of DNA into the E.coli genome via homologous recombination. The DNA of interest is cloned between rbsAR homologous arms and integrated at the rbsAR locus.
Proper citation: RRID:Addgene_46765 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Eric R Geertsma & Bert Poolman; Backbone Size:5824; Vector Backbone:pBADnLIC; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_47075 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Eric R Geertsma & Bert Poolman; Backbone Size:5519; Vector Backbone:pRExLIC; Vector Types:Other, Lactococcus lactis expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_47079 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:LM Guzman; Backbone Size:5780; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_47068 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6300; Vector Backbone:pMSCVpuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_47539 Copy
Species: Synechococcus elongatus PCC 7942
Genetic Insert: PpsbAI
Vector Backbone Description: Vector Backbone:pAM1667; Vector Types:Other, Cyanobacteria cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: NS2.1 vector
Lux Genes: luxCDE
Directs luciferase substrate synthesis in PCC7942
500bp PvuII-SalI promoter fragment of psbAI inserted into SalI-StuI digested pAM1667 (luxCDE).
Proper citation: RRID:Addgene_40242 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Susan Lindquist (Addgene plasmid# 14140); Backbone Size:6972; Vector Backbone:pAG306GPD-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: This plasmid was generated by ligation of a SmaI-digested fusion PCR product that contained two ~500-base-pair regions of chromosome 1 flanking a NotI site into AatII-digested pAG306-GPD-ccdB.
Proper citation: RRID:Addgene_41894 Copy
Species:
Genetic Insert: TAP Tag::mTFP1
Vector Backbone Description: Backbone Marker:Eipcentre; Backbone Size:8353; Vector Backbone:pCC1Fos; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Single-copy vector under non-induced condition. Copy-number-inducible with arabinose enables transient induction of copy-number for DNA isolation, etc.
Proper citation: RRID:Addgene_42161 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R1-R2)
References:
Comments:
Proper citation: RRID:Addgene_19635 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R1-R2)
References:
Comments: Sequence mismatches between Addgene sequence and depositor sequence do not affect function.
Proper citation: RRID:Addgene_19637 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R4-R3)
References:
Comments: Sequence mismatches between Addgene sequence and depositor sequence do not affect function.
Proper citation: RRID:Addgene_19639 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R1-R2)-iA-FLP::let-858(3’)-(Cbr)unc-119(+)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pGC180; Vector Types:Worm Expression, Other, FLP/FRT; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19618 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R1-R2)-L4440
Vector Backbone Description: Backbone Size:0; Vector Backbone:L4440; Vector Types:RNAi, Other, Gateway destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_19680 Copy
Species: Caenorhabditis elegans
Genetic Insert:
References:
Comments:
Proper citation: RRID:Addgene_19671 Copy
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