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Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC57-BsaI; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153256 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC57-BsaI; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153250 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC57-BsaI; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153266 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC57-BsaI; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153263 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC57-BsaI; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153268 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC57-BsaI; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153262 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC57-BsaI; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153261 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC57-BsaI; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153274 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC57-BsaI; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153272 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC57-BsaI; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153271 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC57-BsaI; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.31.018671v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_153270 Copy
Species: Synthetic
Genetic Insert: iRFP713
Vector Backbone Description: Vector Backbone:pPB-tetpA (Addgene #140763); Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_154882 Copy
Species:
Genetic Insert: Gateway cassette
Vector Backbone Description: Backbone Size:12000; Vector Backbone:RCAS (A); Vector Types:Retroviral, RNAi, Other, Avian expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: The RCAS-RNAi viruses have been modified so that they are now compatible with the Gateway cloning system (Invitrogen). DNA encoding the hairpin is cloned into a modified U6 vector using standard cloning techniques. The construct is then moved into the retroviral vector using Gateway reagents, thus bypassing the difficulties of cloning small fragments into RCAS. As a result, each construct can be made with a minimal number of cloning steps. See Supplemental Information of the article for a detailed protocol.
Proper citation: RRID:Addgene_15182 Copy
Species: Synthetic
Genetic Insert: mRFP-T2A-BlasticidinR
Vector Backbone Description: Vector Backbone:modified PB-CA (Addgene 20960) ; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_137881 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pmCellFree_KA2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_145370 Copy
Species: Synthetic
Genetic Insert: PGK-BSD-pA cassette, inserted in PB-TET vector
Vector Backbone Description: Backbone Marker:Andras Nagy (Addgene plasmid # 20909); Backbone Size:12702; Vector Backbone:PB-TET (addgene #20909); Vector Types:Mammalian Expression, piggyBac; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_139562 Copy
Species: Synthetic
Genetic Insert: PGK-ZeoR-pA cassette, inserted in PB-TET vector
Vector Backbone Description: Backbone Marker:Andras Nagy (Addgene plasmid # 20909); Backbone Size:12956; Vector Backbone:PB-TET (addgene #20909); Vector Types:Mammalian Expression, piggyBac; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_139563 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Andras Nagy (Addgene plasmid # 20909); Backbone Size:6112; Vector Backbone:PB-TET (addgene #20909); Vector Types:Mammalian Expression, piggyBac; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: ccdB-Cm cassette was inserted between PB sequences
Proper citation: RRID:Addgene_139518 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Clone cDNA into vector using a Gateway LR reaction. Transform verified plasmid into an expression strain (e.g. BL21(DE3)pLysS) for recombinant protein production.
Proper citation: RRID:Addgene_139787 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:4723; Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_134979 Copy
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