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Species: Mus musculus
Genetic Insert: MCK promoter
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescriptKS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: A 6.5 kb XhoI fragment containing 3 kb of the MCK promoter and enhancer 1, untranslated exon 1, 3 kb of intron 1 including the enhancer region E2, and the first 16 bp of exon 2 was subcloned into the XhoI site of pBluescriptKS to result in pBSMCK. A PolyA sequence was added downstream (see author's map, MCK-polyA-2). Please note that the restriction sites shown between the MCK promoter and polyA region in the author's map are NOT unique sites.
Proper citation: RRID:Addgene_12528 Copy
Species: Mus musculus
Genetic Insert: CCAAT/Enhancer-binding Protein beta
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The C/EBPbeta gene encodes 3 in-frame translational isoforms (LAP*, LAP, and LIP). LAP is the predominant activating isoform expressed in most cells, and this plasmid is designed to express the LAP isoform of the protein. The construct does not include the extra 21 codons at the N terminus that are present in LAP*. The complete insert (not just the ORF) includes a Kozak box sequence at the N terminus.
For more information on the C/EBPbeta isoforms, please see the original reference:
Descombes, P., and Schibler, U. A liver-enriched transcriptional activator protein, LAP, and a transcriptional inhibitory protein, LIP, are translated from the same mRNA. Cell 67:569-579 (1991).
Proper citation: RRID:Addgene_12557 Copy
Species: Mus musculus
Genetic Insert: Zif268 in pB1H1
Vector Backbone Description: Backbone Size:3527; Vector Backbone:pB1H1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_12612 Copy
Species: Mus musculus
Genetic Insert: VCP T317Q
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_12348 Copy
Species: Mus musculus
Genetic Insert: VCP R313K
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: *Please note: Addgene's quality control sequencing results identified an I206V amino acid residue substitution in the VCP sequence when compared to GenBank reference NP_033529.3.
Proper citation: RRID:Addgene_12345 Copy
Species: Mus musculus
Genetic Insert: VCP E305Q
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_12344 Copy
Species: Mus musculus
Genetic Insert: VCP E319Q
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_12352 Copy
Species: Mus musculus
Genetic Insert: autocrine motility factor receptor
Vector Backbone Description: Backbone Size:3766; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_123591 Copy
Species: Mus musculus
Genetic Insert: limb region 1 like
Vector Backbone Description: Backbone Size:5073; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_123593 Copy
Species: Mus musculus
Genetic Insert: limb region 1 like
Vector Backbone Description: Backbone Size:5069; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_123594 Copy
Species: Mus musculus
Genetic Insert: ubiquitin associated domain containing 2
Vector Backbone Description: Backbone Size:5079; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_123597 Copy
Species: Mus musculus
Genetic Insert: Fas associated factor family member 2
Vector Backbone Description: Backbone Size:5079; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_123599 Copy
Species: Mus musculus
Genetic Insert: ubiquitin associated domain containing 2
Vector Backbone Description: Backbone Size:5082; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_123598 Copy
Species: Mus musculus
Genetic Insert: frizzled class receptor 6
Vector Backbone Description: Backbone Size:5079; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_123588 Copy
Species: Mus musculus
Genetic Insert: dishevelled segment polarity protein 2
Vector Backbone Description: Backbone Size:5079; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_123587 Copy
Species: Mus musculus
Genetic Insert: VCP W551A F552A
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_12360 Copy
Species: Mus musculus
Genetic Insert: valosin containing protein
Vector Backbone Description: Backbone Size:5034; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_123600 Copy
Species: Mus musculus
Genetic Insert: VCP W551A
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_12358 Copy
Species: Mus musculus
Genetic Insert: VCP F552A
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: Please note that Addgene's sequencing results identified a I206V mutation in the VCP sequence when compared to GenBank reference NP_033529.3
Proper citation: RRID:Addgene_12359 Copy
Species: Mus musculus
Genetic Insert: VCP R322K
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_12354 Copy
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