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Species: Gallus gallus
Genetic Insert: islet1
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: To make a sense probe, cut with XhoI and transcribe with T3. To make an antisense probe, cut with NotI transcribe with T7.
Proper citation: RRID:Addgene_16273 Copy
Species: Gallus gallus
Genetic Insert: LH-2A
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_16280 Copy
Species: Gallus gallus
Genetic Insert: Hoxa7
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pCAGGs; Vector Types:Other; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_45554 Copy
Species: Gallus gallus
Genetic Insert: mutated C-terminal portion of calmodulin gene (M76-K148)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2745; Vector Backbone:pEXP5-NT/TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_41715 Copy
Species: Gallus gallus
Genetic Insert: mutated calmodulin gene
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2745; Vector Backbone:pEXP5-NT/TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_42573 Copy
Species: Gallus gallus
Genetic Insert: OVA
Vector Backbone Description: Backbone Marker:Kedes Lab; Backbone Size:10200; Vector Backbone:pHBetaAPr-1-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The OVA cDNA was removed from pOVA(230) (McReynolds et al., 1978) by digestion with EcoRI and subcloned into the polylinker of pUG-1. This provided convenient BglII and HindIII restriction sites adjacent to the ovalbumin cDNA for direct subcloning into the BamHI-HindIII site of the pHBetaAPr-1-neo express vector.
Proper citation: RRID:Addgene_22533 Copy
Species: Gallus gallus
Genetic Insert: FGF8
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK (-); Vector Types:Other, Bacterial; Bacterial Resistance:Ampicillin
References:
Comments: Chick homolog of mouse FGF8 splice variant 1 clone was isolated from an E10 chick brain cDNA library directionally cloned into lambdaZapII. The insert contains most of the coding region.
For antisense probe linearize with EcoRI and use T7 Polymerase.
Proper citation: RRID:Addgene_22089 Copy
Species: Gallus gallus
Genetic Insert: Sprouty 1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Other, Bacterial; Bacterial Resistance:Ampicillin
References:
Comments: The partial clone contains ~230bp coding region and 3' UTR sequences.
For antisense probe, linearize the plasmid with XbaI and use T3 Polymerase. For negative control sense probe linearize with XhoI and transcribe with T7 Polymerase.
Proper citation: RRID:Addgene_22095 Copy
Species: Gallus gallus
Genetic Insert: Sprouty 2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK; Vector Types:Other, Bacterial; Bacterial Resistance:Ampicillin
References:
Comments: Partial chicken Sprouty2 cDNA clone was isolated from a chick embryonic brain cDNA library (Lambda Zap). The entire coding region and 3' UTR sequences are included.
For antisense probe, linearize the plasmid with EcoRI and use T7 Polymerase. The transcript should be approximately 1.4kb.
Proper citation: RRID:Addgene_22096 Copy
Species: Gallus gallus
Genetic Insert: TUBB3
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript SK(+); Vector Types:Other, cDNA for in situ hybridization; Bacterial Resistance:Ampicillin
References:
Comments: For riboprobes, cut with EagI. Runoffs with T7.
Proper citation: RRID:Addgene_31232 Copy
Species: Gallus gallus
Genetic Insert: SHH
Vector Backbone Description: Backbone Marker:A. D. Johnson, P. A. Krieg, Dev. Genet. 17, 280 (1995); Vector Backbone:pXeX; Vector Types:Other, Xenopus expression vector; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_31233 Copy
Species: Gallus gallus
Genetic Insert: DISP1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3950; Vector Backbone:pCRII-TOPO; Vector Types:Other, cDNA for in situ hybridization; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: For riboprobe cut with SpeI. Runoffs with T7 polymerase. This is not full length ORF.
Proper citation: RRID:Addgene_31235 Copy
Species: Gallus gallus
Genetic Insert: OVA
Vector Backbone Description: Backbone Marker:Reff et al., 1994; Backbone Size:9209; Vector Backbone:TCAE (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: *The backbone (TCAE) was modified to eliminate the immunoglobulin variable and constant regions, the neomycin resistance gene, and the DHFR gene. See paper for more details.
Proper citation: RRID:Addgene_31598 Copy
Species: Gallus gallus
Genetic Insert: AP-SEMA3C
Vector Backbone Description: Backbone Marker:modified from PCDNA3 see methods in associated publication.; Backbone Size:6000; Vector Backbone:pAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_29449 Copy
Species: Gallus gallus
Genetic Insert: AP-SEMA3A (chick)
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_29448 Copy
Species: Gallus gallus
Genetic Insert: SEMA3A (chick)
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:3000; Vector Backbone:pBLUESCRIPT (SK); Vector Types:Other, pBlue for ISH; Bacterial Resistance:Ampicillin
References:
Comments: To generate the sense probe for ISH, linearize the plasmid with XbaI and transcribe with T3 RNA polymerase. To generate the antisense probe, linearize the plasmid with NotI and transcribe with T7 RNA polymerase.
Please note that T7 is the 5' sequencing primer for the vector; however, the SEMA3A insert is cloned in reverse orientation. For cloning purposes, NotI is at the 5' end of SEMA3A while XbaI is at the 3' end of SEMA3A.
Proper citation: RRID:Addgene_29564 Copy
Species: Gallus gallus
Genetic Insert: 1XDR4
Vector Backbone Description: Backbone Marker:Mark Emerson; Backbone Size:6610; Vector Backbone:Stagia3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Note that there are some minor discrepancies between Addgene's quality control sequence and the depositor's assembled sequence. These differences should not affect plasmid function.
Proper citation: RRID:Addgene_32614 Copy
Species: Gallus gallus
Genetic Insert: Thyroid Response Element
Vector Backbone Description: Backbone Marker:Mark Emerson; Backbone Size:6610; Vector Backbone:Stagia3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_32612 Copy
Species: Gallus gallus
Genetic Insert: rGH-PAL
Vector Backbone Description: Backbone Marker:Mark Emerson; Backbone Size:6610; Vector Backbone:Stagia3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_32617 Copy
Species: Gallus gallus
Genetic Insert: v-src
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pEVX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_17670 Copy
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