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Species: Mus musculus
Genetic Insert: UBP43
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_12454 Copy
Species: Mus musculus
Genetic Insert: Soma-localized ChrimsonR
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:3000; Vector Backbone:pcDNA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124603 Copy
Species: Mus musculus
Genetic Insert: C1V1
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:4000; Vector Backbone:pcDNA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124650 Copy
Species: Mus musculus
Genetic Insert: Tmem98
Vector Backbone Description: Backbone Marker:https://www.addgene.org/19407/; Backbone Size:4200; Vector Backbone:pTretight-2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/512426v1 for BioRxiv preprint
Proper citation: RRID:Addgene_124506 Copy
Species: Mus musculus
Genetic Insert: Tmem98
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pAC-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: The full length Tmem98 coding sequence was initially fused to AC-GFP (Clontech, pAC-GFP-N1 vector).
To generate an sGFP2 fusion, the pAC-GFP-Tmem98 construct was cut with BamHI and BsrgI (to release AC-GFP), after which a BamHI-BsrgI sGFP2 fragment was re-inserted.
The mTurquoise2 tagged variant was generated by exchanging an AgeI/BsrgI sGFP2 fragment for an AgeI/BsrgI mTurquoise fragment. Please visit https://www.biorxiv.org/content/10.1101/512426v1 for BioRxiv preprint
Proper citation: RRID:Addgene_124504 Copy
Species: Mus musculus
Genetic Insert: Murine IgA
Vector Backbone Description: Backbone Size:4706; Vector Backbone:pHybr_r2a; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, Other, HDR template; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_124806 Copy
Species: Mus musculus
Genetic Insert: Murine IgG2b
Vector Backbone Description: Backbone Size:4706; Vector Backbone:pHybr_r2a; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, Other, HDR template; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_124804 Copy
Species: Mus musculus
Genetic Insert: HH5-1 TCRa Tg
Vector Backbone Description: Vector Backbone:pTa; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_124995 Copy
Species: Mus musculus
Genetic Insert: p53
Vector Backbone Description: Backbone Size:5010; Vector Backbone:pLCR0; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid, containing
the wild type murine p53 cDNA under the control of the previously
described BPV LCR-SV40 hybrid promoter (Spaholz et al., 1985),
was constructed as follows. Plasmid p407-1 (Spaholz et al., 1985) was
digested with EcoRI and HindIII, and the 5010-base pair fragment
containing the LCR-SV40 hybrid promoter was isolated and filled in
with Klenow.
After the ligation of BamHI linkers to the ends of the fragment, regenerating the terminal EcoRI site, the fragment was digested with
BamHI and ligated to the 2334-base pair BamHI fragment from the p53 cDNA expression vector pll-4 (Tan et al., 1986). This BamHI
fragment contained the entire wild type murine p53 coding region, 50 base pairs of 5'-untranslated sequence, 287 base pairs of 3'-untranslated
sequence, and a 3'-located SV40 mRNA splice site and polyA signal.
Proper citation: RRID:Addgene_12136 Copy
Species: Mus musculus
Genetic Insert: Pdx1 promoter PH2 domain
Vector Backbone Description: Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_12163 Copy
Species: Mus musculus
Genetic Insert: Dnmt1 shRNA
Vector Backbone Description: Backbone Size:7560; Vector Backbone:pSico; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
References:
Comments: Cre TURNS ON Dmnt1 shRNA expression. The shRNA is directed against the mouse Dnmt1 sequence 5'-GAGTGTGTGAGGGAGAAA-3'.
Proper citation: RRID:Addgene_12165 Copy
Species: Mus musculus
Genetic Insert: MEKK1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1/HisB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please note that Addgene's sequencing results identified a V1467L mutation in the MEKK1 sequence when compared to GenBank ID AF117340.1. The depositing laboratory states that this plasmid functions exactly as intended and the mutation may represent a valid polymorphism.
Proper citation: RRID:Addgene_12181 Copy
Species: Mus musculus
Genetic Insert: MEKK2
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_12183 Copy
Species: Mus musculus
Genetic Insert: MEKK2
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_12182 Copy
Species: Mus musculus
Genetic Insert: IL-4 promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:na; Vector Types:Bacterial Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: Murine IL-4 promotor from -804 to -3 relative to the transcription start site.
Proper citation: RRID:Addgene_12195 Copy
Species: Mus musculus
Genetic Insert: IL-2 promoter
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:0; Vector Backbone:pT81Luc; Vector Types:Bacterial Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: Murine IL-2 promotor from -585 to -34 relative to the transcription start site.
Proper citation: RRID:Addgene_12194 Copy
Species: Mus musculus
Genetic Insert: Siah2
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_122041 Copy
Species: Mus musculus
Genetic Insert: Smarca4
Vector Backbone Description: Backbone Marker:Carmen Williams; Backbone Size:3059; Vector Backbone:pIVT2; Vector Types:Other, For in vitro transcription; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_122146 Copy
Species: Mus musculus
Genetic Insert: Myocardin
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pShuttle; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Myocardin contains an A435T difference compared to the Myocd NCBI entry. The functional consequence of this difference is unknown.
Proper citation: RRID:Addgene_122122 Copy
Species: Mus musculus
Genetic Insert: Wnt3A
Vector Backbone Description: Backbone Size:9220; Vector Backbone:pAdTrack-CMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_12518 Copy
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