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Species: Gallus gallus
Genetic Insert: Op
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3015; Vector Backbone:pGEM-T Easy; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: 645 bp RT-PCR product corresponding to AA 34-260 of sequenced published by Castagnola et al., 1991.
*Note that these mutations were not reported but were found during Addgene's quality control sequencing. They are not known to affect function and may only be polymorphisms.
Proper citation: RRID:Addgene_13941 Copy
Species: Gallus gallus
Genetic Insert: Ptc2
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS SK-; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: 1.42kb partial cDNA insert was obtained from a chick limb bud library that was screened with a mouse ptc-2 probe from ontogeny.
Proper citation: RRID:Addgene_13942 Copy
Species: Gallus gallus
Genetic Insert: BspII
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3015; Vector Backbone:pGEM-T Easy; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: 530 bp RT-PCR product corresponding to amino acids 79-257 of protein sequence published by Yang et al., 1995.
Proper citation: RRID:Addgene_13940 Copy
Species: Gallus gallus
Genetic Insert: Wnt-14
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3015; Vector Backbone:pGEM-T Easy; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Full-length ORF of chick Wnt14 with NcoI linker at 5’ end & EcoRI linker at the 3’ end.
Proper citation: RRID:Addgene_13947 Copy
Species: Gallus gallus
Genetic Insert: Wnt-14
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, Other, avian expression; Bacterial Resistance:Ampicillin
References:
Comments: Full-length ORF of chick Wnt14.
Proper citation: RRID:Addgene_13948 Copy
Species: Gallus gallus
Genetic Insert: Gli3
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Starts at ~aa 430 of hGli3/mGli3.
Proper citation: RRID:Addgene_13954 Copy
Species: Gallus gallus
Genetic Insert: Gli2
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: For in situ probe.
Proper citation: RRID:Addgene_13955 Copy
Species: Gallus gallus
Genetic Insert: Nkx2.5
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(B); Vector Types:Retroviral, Other, avian expression; Bacterial Resistance:Ampicillin
References:
Comments: Entire coding region of Nkx5.2.
Proper citation: RRID:Addgene_13952 Copy
Species: Gallus gallus
Genetic Insert: Dachshund1
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS SK+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: For in situ probe, lacks the 5' end.
Proper citation: RRID:Addgene_13959 Copy
Species: Gallus gallus
Genetic Insert: Tbx5
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, Other, avian expression; Bacterial Resistance:Ampicillin
References:
Comments: Full-length Tbx5.
Proper citation: RRID:Addgene_13963 Copy
Species: Gallus gallus
Genetic Insert: Tbx5
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, Other, avian expression; Bacterial Resistance:Ampicillin
References:
Comments: Contains amino acids 1-274 of Tbx5 fused to a duplex of the lambda hinge region and VP16 (transcriptional activator).
Proper citation: RRID:Addgene_13969 Copy
Species: Gallus gallus
Genetic Insert: Wnt5b
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, Other, avian expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_13975 Copy
Species: Gallus gallus
Genetic Insert: Radical Fringe
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, Other, avian expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_13981 Copy
Species: Gallus gallus
Genetic Insert: Radical Fringe
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(B); Vector Types:Retroviral, Other, avian expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_13982 Copy
Species: Gallus gallus
Genetic Insert: Lunatic Fringe
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(B); Vector Types:Retroviral, Other, avian expression; Bacterial Resistance:Ampicillin
References:
Comments: Flag epitope is a Nhe I - BamHI fragment attached to 3' end of lunatic fringe.
Proper citation: RRID:Addgene_13980 Copy
Species: Gallus gallus
Genetic Insert: cOVA
Vector Backbone Description: Backbone Size:8533; Vector Backbone:pLVX; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_135073 Copy
Species: Gallus gallus
Genetic Insert: Pax6
Vector Backbone Description: Backbone Size:11500; Vector Backbone:RCAS (A); Vector Types:Retroviral, Other, avian; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_15016 Copy
Species: Gallus gallus
Genetic Insert: PLA2G4A-C2 domain
Vector Backbone Description: Vector Backbone:pSUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_170753 Copy
Species: Gallus gallus
Genetic Insert: LH2B
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: full length LH-2B.
Proper citation: RRID:Addgene_16271 Copy
Species: Gallus gallus
Genetic Insert: ER81
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: To make an antisense probe, cut with NotI and transcribe with T7.
Proper citation: RRID:Addgene_16274 Copy
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