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Species: Homo sapiens
Genetic Insert: DNA fragmentation factor subunit alpha
Vector Backbone Description: Vector Backbone:pETDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please note- insertion of amino acids ENLYFG after AA117, and insertion of ENLYFQSG after AA224 in ICAD [Genbank sequence NP_004392.1.] are expected insertions. They make the ICAD variant recognizable and cleavable by TEVP.
Proper citation: RRID:Addgene_100098 Copy
Species: Synthetic
Genetic Insert: ChR1-VChR1 Chimera
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments: Penn Vector Core PV2670
Proper citation: RRID:Addgene_100061 Copy
Species: Synthetic
Genetic Insert: Twitch2B
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments: Penn Vector Core p3409
Proper citation: RRID:Addgene_100040 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Cermak et al. 2011; Vector Backbone:pHD1; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_100044 Copy
Species: Other
Genetic Insert: channelrhodopsin-2
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments: Penn Vector Core PV1959
Proper citation: RRID:Addgene_100054 Copy
Species: Synthetic
Genetic Insert: teLuc, a NanoLuc mutant
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please see https://med.virginia.edu/ai-lab/samples/ for Practical Notes describing the use of this plasmid.
Proper citation: RRID:Addgene_100026 Copy
Species: Synthetic
Genetic Insert: Antares2
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please see https://med.virginia.edu/ai-lab/samples/ for Practical Notes describing the use of this plasmid.
Proper citation: RRID:Addgene_100027 Copy
Species: Other
Genetic Insert: RhoGC E254D
Vector Backbone Description: Vector Backbone:pMT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Alanine insertion at the second position of the RhoGC amino acid sequence does not affect function. Mutation numbering is relative to the wild type reference sequence [KF309499].
Proper citation: RRID:Addgene_100033 Copy
Species: Other
Genetic Insert: RhoAC
Vector Backbone Description: Vector Backbone:pMT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Alanine insertion at the second position of the RhoGC amino acid sequence does not affect function. Mutation numbering is relative to the wild type reference sequence [KF309499].
Proper citation: RRID:Addgene_100032 Copy
Species: Other
Genetic Insert: RhoGC
Vector Backbone Description: Vector Backbone:pMT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Alanine insertion at the second position of the RhoGC amino acid sequence does not affect function.
Proper citation: RRID:Addgene_100031 Copy
Species: Other
Genetic Insert: RhoGC D380N
Vector Backbone Description: Vector Backbone:pMT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Alanine insertion at the second position of the RhoGC amino acid sequence does not affect function. Mutation numbering is relative to the wild type reference sequence [KF309499].
Proper citation: RRID:Addgene_100034 Copy
Species: Synthetic
Genetic Insert: ArcLightDco
Vector Backbone Description: Vector Backbone:pENN.AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments: Penn Vector Core p3381
Proper citation: RRID:Addgene_100038 Copy
Species: Arabidopsis thaliana
Genetic Insert: Phytochrome B
Vector Backbone Description: Backbone Marker:Junichi Miyazaki (Osaka University, Japan); Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Expression vector of PhyB-mCherry-HRasCT in mammalian cells. Of note, the expression level of PIF3-mEGFP must be comparable or lower than that of PhyB. We usually co-transfect pCAGGS-PhyB-mCherry-HRasCT and pCAGGS-PIF3-mEGFP plasmids into HeLa cells with lipofection in a 50:1 ratio.
mCherry has a G229L mutation that does not affect function.
Proper citation: RRID:Addgene_100281 Copy
Species: Other
Genetic Insert: MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
Vector Backbone Description: Backbone Marker:Junichi Miyazaki (Osaka University, Japan); Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Expression vector for Phycocyanobilin (PCB) synthesis in mammalian cells.
Proper citation: RRID:Addgene_100280 Copy
Species: Other
Genetic Insert: MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
Vector Backbone Description: Backbone Marker:Junichi Miyazaki (Osaka University, Japan); Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Expression vector for Phycocyanobilin (PCB) synthesis in mammalian cells. In addition, shRNA targeting to mouse BVRA is expressed so that PCB is further accumulated.
Proper citation: RRID:Addgene_100286 Copy
Species: Other
Genetic Insert: Concentrative nucleoside transporter
Vector Backbone Description: Vector Backbone:pET26; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_100164 Copy
Species: Other
Genetic Insert: MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
Vector Backbone Description: Backbone Marker:Junichi Miyazaki (Osaka University, Japan); Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Expression vector for Phycocyanobilin (PCB) synthesis in mammalian cells. In addition, shRNA targeting to human BVRA is expressed so that PCB is further accumulated.
Proper citation: RRID:Addgene_100285 Copy
Species: Other
Genetic Insert: GGPP synthase
Vector Backbone Description: Backbone Size:4846; Vector Backbone:pLM304; Vector Types:Yeast Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Addgene NGS sequencing found the following mutations compared to the provided reference sequence: crtE with M176I, K346R, I347M, E353D mutations, crtI with L107F, H427Q mutations, and crtYB with A190T, E307K mutations. tHMG1 contains only residues 531-1054 compared to the NCBI reference seq [M22002.1]. These mutations/truncation do not affect plasmid function.
Proper citation: RRID:Addgene_100539 Copy
Species: Arabidopsis thaliana
Genetic Insert: Phytochrome B
Vector Backbone Description: Backbone Size:4885; Vector Backbone:pL1A0*B0*_Leu; Vector Types:Yeast Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_100537 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: It is used to standardize new parts for EMMA toolkit. New DNA fragments (parts) are cloned into this plasmid replacing the RFP gene through a BsaI Golden Gate reaction. The resulting plasmid will have the part flanked by BsmBI sites and the fusion sites corresponding to position 13 of EMMA platform.
Proper citation: RRID:Addgene_100623 Copy
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