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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pETDuet_huCAD_ICADL (TevS) Resource Report Resource Website |
RRID:Addgene_100098 | DNA fragmentation factor subunit alpha | Homo sapiens | Ampicillin | PMID:26106156 | Please note- insertion of amino acids ENLYFG after AA117, and insertion of ENLYFQSG after AA224 in ICAD [Genbank sequence NP_004392.1.] are expected insertions. They make the ICAD variant recognizable and cleavable by TEVP. | Vector Backbone:pETDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | two caspase cleavage sites (DETD-117 and DAVD-224) in ICAD were sequentially mutated to TEVP cleavable sequences | 2022-04-22 03:14:47 | 0 |
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pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry (PV2670) Resource Report Resource Website |
RRID:Addgene_100061 | ChR1-VChR1 Chimera | Synthetic | Ampicillin | PMID: | Penn Vector Core PV2670 | Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | E122T and E162T | 2022-04-22 03:14:46 | 0 |
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pAAV.hSyn1.Twitch2B.WPRE.SV40 Resource Report Resource Website |
RRID:Addgene_100040 | Twitch2B | Synthetic | Ampicillin | PMID:24390440 | Penn Vector Core p3409 | Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2022-04-22 03:14:46 | 0 | |
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pYPQ131D2.0 Resource Report Resource Website |
RRID:Addgene_100044 | Tetracycline | PMID:29197638 | Backbone Marker:Cermak et al. 2011; Vector Backbone:pHD1; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Tetracycline | 2022-04-22 03:14:46 | 0 | ||||
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pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 Resource Report Resource Website 1+ mentions |
RRID:Addgene_100054 | channelrhodopsin-2 | Other | Ampicillin | PMID: | Penn Vector Core PV1959 | Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | H134R mutation in ChR2 | 2022-04-22 03:14:46 | 5 |
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pcDNA3-teLuc c-myc Resource Report Resource Website |
RRID:Addgene_100026 | teLuc, a NanoLuc mutant | Synthetic | Ampicillin | PMID:28869756 | Please see https://med.virginia.edu/ai-lab/samples/ for Practical Notes describing the use of this plasmid. | Backbone Marker:Thermo Fisher; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | D19S, D85N, C164H | 2022-04-22 03:14:46 | 0 |
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pcDNA3-Antares2 c-myc Resource Report Resource Website |
RRID:Addgene_100027 | Antares2 | Synthetic | Ampicillin | PMID:28869756 | Please see https://med.virginia.edu/ai-lab/samples/ for Practical Notes describing the use of this plasmid. | Backbone Marker:Thermo Fisher; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:14:46 | 0 | |
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pMT3-RhoGC E254D Resource Report Resource Website |
RRID:Addgene_100033 | RhoGC E254D | Other | Ampicillin | PMID:28473465 | Alanine insertion at the second position of the RhoGC amino acid sequence does not affect function. Mutation numbering is relative to the wild type reference sequence [KF309499]. | Vector Backbone:pMT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | E254D | 2022-04-22 03:14:46 | 0 |
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pMT3-RhoAC Resource Report Resource Website |
RRID:Addgene_100032 | RhoAC | Other | Ampicillin | PMID:28473465 | Alanine insertion at the second position of the RhoGC amino acid sequence does not affect function. Mutation numbering is relative to the wild type reference sequence [KF309499]. | Vector Backbone:pMT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | E497K/C566D | 2022-04-22 03:14:46 | 0 |
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pMT3-RhoGC Resource Report Resource Website |
RRID:Addgene_100031 | RhoGC | Other | Ampicillin | PMID:28473465 | Alanine insertion at the second position of the RhoGC amino acid sequence does not affect function. | Vector Backbone:pMT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:14:46 | 0 | |
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pMT3-RhoGC D380N Resource Report Resource Website |
RRID:Addgene_100034 | RhoGC D380N | Other | Ampicillin | PMID:28473465 | Alanine insertion at the second position of the RhoGC amino acid sequence does not affect function. Mutation numbering is relative to the wild type reference sequence [KF309499]. | Vector Backbone:pMT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | D380N | 2022-04-22 03:14:46 | 0 |
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pENN.AAV.hSynap.Flex.ArcLightDco.WPRE.SV40 Resource Report Resource Website |
RRID:Addgene_100038 | ArcLightDco | Synthetic | Ampicillin | PMID: | Penn Vector Core p3381 | Vector Backbone:pENN.AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2022-04-22 03:14:46 | 0 | |
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pCAGGS-hPhyB-mCherry-HRasCT Resource Report Resource Website |
RRID:Addgene_100281 | Phytochrome B | Arabidopsis thaliana | Ampicillin | PMID:29078307 | Expression vector of PhyB-mCherry-HRasCT in mammalian cells. Of note, the expression level of PIF3-mEGFP must be comparable or lower than that of PhyB. We usually co-transfect pCAGGS-PhyB-mCherry-HRasCT and pCAGGS-PIF3-mEGFP plasmids into HeLa cells with lipofection in a 50:1 ratio. mCherry has a G229L mutation that does not affect function. | Backbone Marker:Junichi Miyazaki (Osaka University, Japan); Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Human codon optimized PhyB (1-908 aa) | 2022-04-22 03:14:48 | 0 |
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pCAGGS-PHFF Resource Report Resource Website |
RRID:Addgene_100280 | MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes) | Other | Ampicillin | PMID:29078307 | Expression vector for Phycocyanobilin (PCB) synthesis in mammalian cells. | Backbone Marker:Junichi Miyazaki (Osaka University, Japan); Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | All genes are codon-optimized for expression in human cells. | 2022-04-22 03:14:48 | 0 |
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pCAGGS-PHFF-sh-mBVRA Resource Report Resource Website |
RRID:Addgene_100286 | MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes) | Other | Ampicillin | PMID:29078307 | Expression vector for Phycocyanobilin (PCB) synthesis in mammalian cells. In addition, shRNA targeting to mouse BVRA is expressed so that PCB is further accumulated. | Backbone Marker:Junichi Miyazaki (Osaka University, Japan); Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | All genes are codon-optimized for expression in human cells. | 2022-04-22 03:14:49 | 0 |
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pET26-CNTN149L Resource Report Resource Website |
RRID:Addgene_100164 | Concentrative nucleoside transporter | Other | Kanamycin | PMID:28424521 | Vector Backbone:pET26; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | N149L | 2022-04-22 03:14:48 | 0 | |
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pCAGGS-PHFF-sh-hBVRA Resource Report Resource Website |
RRID:Addgene_100285 | MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes) | Other | Ampicillin | PMID:29078307 | Expression vector for Phycocyanobilin (PCB) synthesis in mammalian cells. In addition, shRNA targeting to human BVRA is expressed so that PCB is further accumulated. | Backbone Marker:Junichi Miyazaki (Osaka University, Japan); Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | All genes are codon-optimized for expression in human cells. | 2022-04-22 03:14:49 | 0 |
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pLM494 Resource Report Resource Website |
RRID:Addgene_100539 | GGPP synthase | Other | Ampicillin | PMID:29789561 | Addgene NGS sequencing found the following mutations compared to the provided reference sequence: crtE with M176I, K346R, I347M, E353D mutations, crtI with L107F, H427Q mutations, and crtYB with A190T, E307K mutations. tHMG1 contains only residues 531-1054 compared to the NCBI reference seq [M22002.1]. These mutations/truncation do not affect plasmid function. | Backbone Size:4846; Vector Backbone:pLM304; Vector Types:Yeast Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin | 2022-04-22 03:14:52 | 0 | |
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pLH_Scr15 Resource Report Resource Website |
RRID:Addgene_100537 | Phytochrome B | Arabidopsis thaliana | Ampicillin | PMID:29789561 | Backbone Size:4885; Vector Backbone:pL1A0*B0*_Leu; Vector Types:Yeast Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin | N-terminal version of PhyB (AA 1-621)/ Mutation of nucleotide 576 C-->A | 2022-04-22 03:14:52 | 0 | |
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YCe1707 HC_Kan_RFP-p13 Resource Report Resource Website |
RRID:Addgene_100623 | Kanamycin | PMID:28418644 | It is used to standardize new parts for EMMA toolkit. New DNA fragments (parts) are cloned into this plasmid replacing the RFP gene through a BsaI Golden Gate reaction. The resulting plasmid will have the part flanked by BsmBI sites and the fusion sites corresponding to position 13 of EMMA platform. | Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2022-04-22 03:14:53 | 0 |
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