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Species: Homo sapiens
Genetic Insert: ASCL3
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221; Vector Types:Other, Gateway shuttling vector; Bacterial Resistance:Kanamycin
References:
Comments: Harvard generated by gene synthesis
Proper citation: RRID:Addgene_101515 Copy
Species: Homo sapiens
Genetic Insert: HOXB3
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221; Vector Types:Other, Gateway shuttling vector; Bacterial Resistance:Kanamycin
References:
Comments: Harvard generated by gene synthesis
Proper citation: RRID:Addgene_101478 Copy
Species: Other
Genetic Insert: amFP486
Vector Backbone Description: Vector Backbone:pNCS; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: The fluorescent protein sequence comes from the following publication: Matz M. V.; Fradkov A. F.; Labas Y. A.; Savitsky A. P.; Zaraisky A. G.; Markelov M. L.; Lukyanov S. A. Fluorescent Proteins from Nonbioluminescent Anthozoa Species. Nat. Biotechnol. 1999, 17, 969_97310.1038/13657.
Proper citation: RRID:Addgene_101646 Copy
Species: Other
Genetic Insert: EG-4
Vector Backbone Description: Vector Backbone:pNCST; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Created in the lab of Nathan Shaner at the Scintillon Institute.
Proper citation: RRID:Addgene_101640 Copy
Species: Homo sapiens
Genetic Insert: ZNF275
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221; Vector Types:Other, Gateway shuttling vector; Bacterial Resistance:Kanamycin
References:
Comments: Harvard generated by gene synthesis
Proper citation: RRID:Addgene_101487 Copy
Species: Other
Genetic Insert: eqFP486
Vector Backbone Description: Vector Backbone:pNCS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: The fluorescent protein sequence was found at GenBank Accession AF545829.
Proper citation: RRID:Addgene_101641 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.3-TOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_100091 Copy
Species: Homo sapiens
Genetic Insert: DNMT3A
Vector Backbone Description: Backbone Marker:ThermoFisher; Backbone Size:5407; Vector Backbone:pcDNA3.3-TOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_100090 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sgd1
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
References:
Comments: Addgene NGS results found T307A, S332F, and V832F in the Sgd1 translation compared to the best-match NCBI reference sequence (GAA25198.1).
Proper citation: RRID:Addgene_100000 Copy
Species: Homo sapiens
Genetic Insert: SUVAR39H1
Vector Backbone Description: Backbone Marker:ThermoFisher; Backbone Size:5407; Vector Backbone:pcDNA3.3-TOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_100088 Copy
Species: Mus musculus
Genetic Insert: Ezh2[SET]
Vector Backbone Description: Backbone Marker:ThermoFisher; Backbone Size:5407; Vector Backbone:pcDNA3.3-TOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_100087 Copy
Species: Mus musculus
Genetic Insert: 14056
Vector Backbone Description: Backbone Marker:ThermoFisher; Backbone Size:5407; Vector Backbone:pcDNA3.3-TOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_100086 Copy
Species: Homo sapiens
Genetic Insert: FOG1(aa 1-45)
Vector Backbone Description: Backbone Marker:ThermoFisher; Backbone Size:5407; Vector Backbone:pcDNA3.3-TOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_100085 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Dhr1
Vector Backbone Description: Vector Backbone:pACT2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: Addgene NGS results found S1073N, and an extra 16 amino acids at the C-terminus of the Dhr1 translation compared to NP_013847.1.
Proper citation: RRID:Addgene_100003 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Dhr1
Vector Backbone Description: Vector Backbone:pAS2-1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: Addgene NGS results found S1073N, and an extra 30 amino acids at the C-terminus of the Dhr1 translation compared to NP_013847.1.
Proper citation: RRID:Addgene_100002 Copy
Species: Homo sapiens
Genetic Insert: G9A
Vector Backbone Description: Backbone Marker:ThermoFisher; Backbone Size:5407; Vector Backbone:pcDNA3.3-TOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_100089 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:12919; Vector Backbone:pNOC-CRISPR-HA; Vector Types:Other, Algae, Nannochloropsis expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_100008 Copy
Species: Caenorhabditis elegans
Genetic Insert: zif-1
Vector Backbone Description: Backbone Marker:Christian Frøkjær-Jensen (Erik Jorgensen lab); Backbone Size:7328; Vector Backbone:pCFJ151; Vector Types:Other, Targeting vector for Mos1 transposon mediated single copy transgene insertion; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_100006 Copy
Species: Homo sapiens
Genetic Insert: STAR protein
Vector Backbone Description: Backbone Size:4000; Vector Backbone:HMBP-3C; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: The STARD1 domain sequence was subcloned after 3C site to obtain 3C cleavable His6-MBP-3C-STAR construction.
Proper citation: RRID:Addgene_100094 Copy
Species: Homo sapiens
Genetic Insert: 14-3-3 sigma human
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28-his-3C; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: The 3C site was introduced immediately prior to 14-3-3 sequence to facilitate purification by subtractive IMAC.
Proper citation: RRID:Addgene_100093 Copy
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