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Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: Addgene NGS was unable to completely resolve all bases in the CloDF13 Origin. The plasmid has been successfully propagated at Addgene and these ambiguous bases do not appear to have a functional impact on the plasmid
Proper citation: RRID:Addgene_160262 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: Addgene NGS was unable to completely resolve all bases in the CloDF13 Origin. The plasmid has been successfully propagated at Addgene and these ambiguous bases do not appear to have a functional impact on the plasmid
Proper citation: RRID:Addgene_160171 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: Addgene NGS was unable to completely resolve all bases in the CloDF13 Origin. The plasmid has been successfully propagated at Addgene and these ambiguous bases do not appear to have a functional impact on the plasmid
Proper citation: RRID:Addgene_160169 Copy
Species: Arabidopsis thaliana
Genetic Insert: Telomere
Vector Backbone Description: Backbone Size:9189; Vector Backbone:pTF101.1; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: Addgene was unable to sequence this plasmid due to the repetitive nature of the telomere repeats. The plasmid was instead verified by restriction digest. The gel image is provided on this page under- Resource Information- Addgene Notes.
Proper citation: RRID:Addgene_65721 Copy
Species: Other
Genetic Insert: Transposon Tn5-692
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: Tn5 derivative Tn5-692 (in plasmid pRL692; GenBank accession no. AF424805) contains a pMB1 oriV; and bears mutations (Zhou, et al. 1998 J. Mol. Biol. 276:913–925) that increase its rate of transposition ca. 100-fold relative to that of Tn5-1058 (Wolk, et al. 1991. Proc. Natl. Acad. Sci. USA 88:5355–5359), providing large numbers of transposon mutants. The erythromycin-resistance gene came from Horinouchi and Weisblum (J. Bacteriol. 150: 804-814, 1982) and the streptomycin-spectinomycin gene came from the Ω-fragment described by Prentki and Krisch (Gene 29, 303-313, 1984) and was purchased as an EcoRI fragment from Amersham.
NOTE--Because Tn5-692 appears able to transpose repeatedly, the user is cautioned not to delay storing strains whose phenotype is of interest.
Additional references:
Miyagishima S-y, Wolk CP, Osteryoung KW (2005) Identification of cyanobacterial cell division genes by comparative and mutational analyses. Mol Microbiol 56: 126-143
Proper citation: RRID:Addgene_70284 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:RSF1010; Vector Types:Other, Bacterial shuttle vector; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: MCS: (PT7→)HindIII-SphI-PstI(SbfI)-SalI(HincII)-KpnI-SacI-EcoRI-ClaI-ApaI-SacII-NsiI-NdeI-BglII-AflII-StuI-AvrII-XbaI-AatII-BsiWI-XhoI(PspXI)-SrfI-AscI-RsrII-BamHI(←PSP6)
Proper citation: RRID:Addgene_70692 Copy
Species: Synthetic
Genetic Insert: sgRNA-FRT
Vector Backbone Description: Vector Backbone:pTargetF; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_113637 Copy
Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Keunsub Lee; Backbone Size:6149; Vector Backbone:pTF505; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_187872 Copy
Species:
Genetic Insert: Synpcc7942_1849
Vector Backbone Description: Vector Backbone:Vector for targeting integration at neutral site 1 in ATCC PCC7942 genome; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_119795 Copy
Species:
Genetic Insert: Synpcc7942_1557
Vector Backbone Description: Vector Backbone:Vector for targeting integration at neutral site 1 in ATCC PCC7942 genome; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_119796 Copy
Species: Other
Genetic Insert: pAM4788 where the promoter/reporter device (PconII-LTRBS-yemGFP) was replaced with a MCS from the pBluescript vector (pBS MCS).
Vector Backbone Description: Backbone Size:6936; Vector Backbone:pAM4788; Vector Types:Other, other; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_120087 Copy
Species: Other
Genetic Insert: Tetanus toxin light chain domain
Vector Backbone Description: Backbone Size:4535; Vector Backbone:pTrc99S; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_128402 Copy
Species: Other
Genetic Insert: Exotoxin A
Vector Backbone Description: Backbone Size:4535; Vector Backbone:pTrc99S; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_128404 Copy
Species: Other
Genetic Insert: H. influenzae protein D
Vector Backbone Description: Backbone Size:4535; Vector Backbone:pTrc99S; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_128399 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_190156 Copy
Species: Other
Genetic Insert: kaiBC S431E T432E with p0050 promoter
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pAM1303; Vector Types:Other, Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_101836 Copy
Species: Other
Genetic Insert: sp/st resistance casette followed by ptrc promoter
Vector Backbone Description: Backbone Size:4400; Vector Backbone:pBR322; Vector Types:Other, Cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_101846 Copy
Species: Other
Genetic Insert: RpaA with its native promoter
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pAM1303; Vector Types:Other, Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_101829 Copy
Species: Other
Genetic Insert: RelA fragment from E.coli with point mutation E335Q
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pam1303; Vector Types:Other, Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments:
Proper citation: RRID:Addgene_100917 Copy
Species: Synechococcus elongatus PCC 7942
Genetic Insert: One Step Cloneing Vector for Overexpression
Vector Backbone Description: Vector Backbone:pAM2990; Vector Types:Other, Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin
References:
Comments: pAM2990 was partially digested with SacI and re-ligatedto remove the antihel fragment. The net result is the insertion of the BglII fragment from pAM2255 into BamHI digested pAM1303 in the orientation that Ptrc goes away from the SacI site in pAM1303 (SacI digestion gives a 1.7 KB band). This plasmid can be used as a one-step cloning vector for overexpression.
Proper citation: RRID:Addgene_40248 Copy
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