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Species: Homo sapiens
Genetic Insert: EED
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid #26096); Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: EED:APC049-D10:C27444. SGC or PDB link: http://www.thesgc.org/structures/5TTW/
Proper citation: RRID:Addgene_101254 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:5802; Vector Backbone:pSNAP-tag (m) Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Codon usage of the gene is optimized for expression in mammalian cells. pSNAP-tag(m) contains two multiple cloning sites to allow cloning of the fusion partner as a fusion to the N- or C-terminus of the SNAP-tag. to allow cloning of the fusion partner as a fusion to the N- or C-terminus of the SNAP-tag. For more information on this plasmid, please see https://www.addgene.org/depositor-collections/neb-cell-imaging-tags/
This plasmid is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
Proper citation: RRID:Addgene_101135 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:3725; Vector Backbone:pSNAP-tag (T7) Vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: pSNAP-tag(T7) is a high copy number E. coli expression plasmid encoding SNAP26b, a SNAP-tag protein, which can be expressed under the control of the T7 promoter. Codon usage of the gene is optimized for expression in E. coli. The fusion partner should be cloned as a fusion to the C-terminus of the SNAP-tag. For more information on this plasmid, please see https://www.addgene.org/depositor-collections/neb-cell-imaging-tags/
This plasmid is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
Proper citation: RRID:Addgene_101137 Copy
Species: Other
Genetic Insert: LANApi driving green luciferase reporter for CBG68 in pCBG68 backbone.
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5104; Vector Backbone:pCBG68 Basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101198 Copy
Species: Synthetic
Genetic Insert: hSpCas9-Cluster5+Q926A(K918A/V922A/R925A/Q926A)
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid #43861); Vector Backbone:JDS246; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101192 Copy
Species:
Genetic Insert: ffDronpa-F
Vector Backbone Description: Backbone Size:2860; Vector Backbone:pRSETb; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101193 Copy
Species: Other
Genetic Insert: SpCas9 variant G582A/V583A/E584A/D585A/N588A
Vector Backbone Description: Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint.
Proper citation: RRID:Addgene_101233 Copy
Species: Other
Genetic Insert: SpCas9 variant F491A/M495A/T496A/N497A/Q926A
Vector Backbone Description: Backbone Marker:Doudna lab; Vector Backbone:pCT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint.
Proper citation: RRID:Addgene_101235 Copy
Species: Synthetic
Genetic Insert: LOV2 and STIM1 cytosolic fragment
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pTriEx; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101245 Copy
Species: Mus musculus
Genetic Insert: alpha-Catenin
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: The TS module with mTFP1-F40-venusA206K was adapted from Vinculin TS (a kind gift from Martin Schwartz [Grashoff et al., 2010]). The TSmod was cloned in to pEGFP-N1 vector at Xho1 and Not1 restriction sites. For aE-Cat-TS, the mouse N-terminal (1_697 aa) of alpha-catenin was inserted before mTFP1 by in- fusion cloning (Takara Bioscience, Clontech) at the Xho1 restriction site
Proper citation: RRID:Addgene_101298 Copy
Species: Mus musculus
Genetic Insert: alpha-Catenin
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: The C-terminal fragment (698-906 aa) of alpha-catenin was cloned after VenusA206K at the Not1 restriction site
Proper citation: RRID:Addgene_101299 Copy
Species: Homo sapiens
Genetic Insert: METTL13
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid #62304); Vector Backbone:pFBOH-MHL; Vector Types:Other, Baculovirus Expression; Bacterial Resistance:Ampicillin
References:
Comments: N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: METTL13:PBC007-B09:C235684. SGC or PDB link: http://www.thesgc.org/structures/5WCJ/
Proper citation: RRID:Addgene_101270 Copy
Species: Homo sapiens
Genetic Insert: EPHA3
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid #26094); Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: N terminal tag: MGSSHHHHHHSSGLVPRGS. SGC Clone Sample ID: EPHA3:SDC033-F08:C12121. SGC or PDB link: http://www.rcsb.org/pdb/explore/explore.do?structureId=2QO2
Proper citation: RRID:Addgene_101271 Copy
Species: Other
Genetic Insert: 4PY6
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid #26092); Vector Backbone:pET15-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: PFA0510w:MAC02W-B09:C201930. SGC or PDB link: http://www.rcsb.org/pdb/explore/explore.do?structureId=4PY6
Proper citation: RRID:Addgene_101272 Copy
Species: Other
Genetic Insert: oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
Vector Backbone Description: Vector Backbone:colE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_101316 Copy
Species: Other
Genetic Insert: oriC2 of M. florum strain L1 (dnaA/dnaN intergenic region)
Vector Backbone Description: Vector Backbone:colE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_101310 Copy
Species: Other
Genetic Insert: oriC3 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, w/o dnaA)
Vector Backbone Description: Vector Backbone:colE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_101311 Copy
Species: Homo sapiens
Genetic Insert: Utrophin
Vector Backbone Description: Vector Backbone:pCS2.0; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101279 Copy
Species: Other
Genetic Insert: LANApi TATA box mutation in pBluescript-LANApi,K14 (pDD2000).
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:7150; Vector Backbone:pBluescript II (KS+); Vector Types:Unspecified; Bacterial Resistance:Ampicillin
References:
Comments: Addgene's sequencing found Y108H and G360D mutations in CBG68luc. These mutations are not known to affect plasmid function.
Proper citation: RRID:Addgene_101372 Copy
Species: Other
Genetic Insert: RBPjk binding site B mutation in pBluescript-LANApi,K14 (pDD2000).
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:7150; Vector Backbone:pBluescript II (KS+); Vector Types:Unspecified; Bacterial Resistance:Ampicillin
References:
Comments: Addgene's sequencing found V466A and R510C mutations in CBG68luc and a V298A mutation in CBRluc. These mutations are not known to affect plasmid function.
Proper citation: RRID:Addgene_101373 Copy
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